Corporate Banner
Satellite Banner
Chromatography
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Researchers Find U.S. Breast Milk is Glyphosate Free
Washington State University scientists have found that glyphosate, the main ingredient in the herbicide Roundup, does not accumulate in mother’s breast milk.
Peering into the Vapors
Research suggests that e-cigarettes are much less harmful than previous studies have indicated.
New Technique for Mining Health-conferring Soy Compounds
A new procedure devised by U.S. Department of Agriculture (USDA) scientists to extract lunasin from soybean seeds could expedite further studies of this peptide for its cancer-fighting potential and other health benefits.
Could a simple saliva test detect Alzheimer's?
Researchers have presented findings suggesting that a simple, non-invasive diagnostic for Alzheimer's could be within reach.
Extracting Ions From A Single Drop of Blood
A new, miniature device that extracts trace ions from just one drop of blood with minimal sample preparation could vastly simplify the analysis of blood samples for certain components.
Improving Snake Antivenom
Scientists are working to determine the effectiveness of antivenom to improve treatment options for people who have been bitten by a poisonous snake.
The Perfect Partnership: Research & Industry; Software & Instrumentation. It really starts to come together at ASMS 2015
Collaboration and knowledge-sharing were evident everywhere: on the bus, in the hallways and in the bars. This article aims to capture this theme and share with you some of the fruits of this coming together of science and industry.
Researchers Use Nanotubes to Better Understand Diseases
Researchers in UC’s Department of Cancer Biology are collaborating with material scientists from the University of Houston to create and use nanotubes to capture and understand the regulation of proteins involved in a variety of diseases.
3D for Top-Down Proteomics: Extra Dimension for More Proteins
Researchers from the University of Wisconsin- Madison have tested the possible applications of adding a "third dimension" to chromatography.
Scientists Come Up with New Technique to Detect Alien Life on Mars
Researchers have provided evidence that combining chromatography/mass spectroscopy with Raman spectroscopy could improve astrobiological studies.
Scroll Up
Scroll Down

Automated Sample Preparation of 25-OH-Vitamin D3/D2 from Serum with the AC Extraction Plate and the Tecan Freedom EVO® for the quantification by LC-MS/MS
Bookmark and Share

Tecan Group Ltd.

The current methods for sample preparation include protein precipitation (‘protein crash’), solvent extraction such as liquid-liquid extraction (LLE) and solid-phase extraction (SPE). 

However, all these methods require at least one labor intensive and time-consuming step such as filtration, centrifugation or solvent evaporation. In addition, these steps do not allow straightforward automation of the sample preparation process which is highly desirable in order to cope with the increasing Vitamin D sample load experienced by clinical laboratories. 

The Tecan AC Extraction Plate simplifies the sample preparation step in such a way that no centrifugation, filtration or solvent evaporation steps are needed. In fact the sample preparation procedure is reduced to simple pipetting and shaking. These pipetting and shaking steps were automated on a Tecan Freedom EVO liquid handling system. 

In this application note an approach is described which overcomes the difficulties described above. 


Further Information


Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!