Thursday, February 23, 2012
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 | Old Structures - New Targets for Antivirulence Salicylidene Acylhydrazide Compounds Mads Gabrielsen, Post Doc, University of Glasgow, speaking at Advances in Protein Crystallography 2010. |
 | Novel Methodology for Improving Crystal Quality in Microbatch Sahir Khurshid, Post Doc, Imperial College London, speaking at Advances in Protein Crystallography 2010. |
Novel Antibodies & Therapeutic Proteins: Technological Challenges & Future Prospects
Select Biosciences Ltd
This innovative & analytical report can be used to assess market trends & appraise the potential of new antibodies & therapeutic proteins. It provides perspective to gain an in-depth understanding of the marketplace, players, products & leading technologies driving the field.
Molecular Diagnostics Market: Trends and Opportunities
Koncept Analytics
Molecular diagnostics or the analysis of DNA and RNA at the molecular level - is a fast-growing market, driven by the growing understanding of the human genome. Although a small part of the In-Vitro Diagnostics (IVD) market, molecular diagnostics market is one of the two growth drivers of the IVD industry. In the decision making process of healthcare experts, diagnostics tests have a major influence.
Multiplexed Diagnostics 2010
Select Biosciences Ltd
The multiplexed diagnostics market has grown rapidly and generated sales of approximately $2.4 billon in 2009, but is now balanced on multiple tipping points.
Select Biosciences Ltd
This innovative & analytical report can be used to assess market trends & appraise the potential of new antibodies & therapeutic proteins. It provides perspective to gain an in-depth understanding of the marketplace, players, products & leading technologies driving the field.
Molecular Diagnostics Market: Trends and Opportunities
Koncept Analytics
Molecular diagnostics or the analysis of DNA and RNA at the molecular level - is a fast-growing market, driven by the growing understanding of the human genome. Although a small part of the In-Vitro Diagnostics (IVD) market, molecular diagnostics market is one of the two growth drivers of the IVD industry. In the decision making process of healthcare experts, diagnostics tests have a major influence.
Multiplexed Diagnostics 2010
Select Biosciences Ltd
The multiplexed diagnostics market has grown rapidly and generated sales of approximately $2.4 billon in 2009, but is now balanced on multiple tipping points.
NIH Common Fund Researchers Uncover Structure of Important Target for Drug Design
Researchers have uncovered a potent class of small molecules that selectively turn on the S1P1 receptor.
Structure of Complex Zeolite explains its Role in Naphtha-to-Diesel Conversion
Using electron crystallography scientists have determined that a new zeolite has a unique structure containing discrete pores of different dimensions that are connected together.
Protein Dynamics at Eph Receptor-Ligand Interfaces as Revealed by Crystallography, NMR and MD Simulations
The research highlights the key role of protein dynamics in Eph-ephrin signalling, demonstrating how this would benefit future design of agonists/antagonists targeting Eph receptors.
X-ray Diffraction of Protein Crystal Grown in a Nano-liter Scale Droplet in a Microchannel and Evaluation of Its Applicability
Researchers from Kyushu University describe the technical aspects of the in-situ X-ray diffraction of a protein crystal prepared by a nanodroplet-based crystallization method.
Crystal Structure of Urea Carboxylase Provides Insights into the Carboxyltransfer Reaction
Research from the Chinese Academy of Sciences could shed light on the mechanism of these enzymes' catalysis.
Vitamins could lead to the Design of Novel Drugs to Combat Malaria
The research will enable scientists to learn more about the nature of the enzymes required for vitamin biosynthesis by the malaria causing pathogen Plasmodium.
A New Generation of Crystallographic Validation Tools for the Protein Data Bank
This report presents the conclusions of the X-ray Validation Task Force of the worldwide Protein Data Bank.
Temperature Controlled Stage Used in Crystallisation Processes
Linkam Scientific Instrument report on the work of Professor Yves Henri Geerts from the Université Libre de Bruxelles where he uses a specially designed temperature stage to study crystallisation processes in opto-electronic thin films.
CSHL Team Solves a Protein Complex’s Molecular Structure to Explain Role in Gene Silencing
Scientists from Cold Spring Harbor Laboratory and their collaborators at St. Jude’s Research Hospital have discovered new details of how various domains of the protein complex contribute to heterochromatin assembly and gene silencing.
Noncovalent Polymerization of Mesogens Crystallizes Lysozyme: Correlation Between Nonamphiphilic Lyotropic Liquid Crystal Phase and Protein Crystal Formation
Researchers from Syracuse University we report the use of organic salts that can form a class of unusual nonamphiphilic lyotropic liquid crystals to crystallize the protein lysozyme.
Researchers have uncovered a potent class of small molecules that selectively turn on the S1P1 receptor.
Structure of Complex Zeolite explains its Role in Naphtha-to-Diesel Conversion
Using electron crystallography scientists have determined that a new zeolite has a unique structure containing discrete pores of different dimensions that are connected together.
Protein Dynamics at Eph Receptor-Ligand Interfaces as Revealed by Crystallography, NMR and MD Simulations
The research highlights the key role of protein dynamics in Eph-ephrin signalling, demonstrating how this would benefit future design of agonists/antagonists targeting Eph receptors.
X-ray Diffraction of Protein Crystal Grown in a Nano-liter Scale Droplet in a Microchannel and Evaluation of Its Applicability
Researchers from Kyushu University describe the technical aspects of the in-situ X-ray diffraction of a protein crystal prepared by a nanodroplet-based crystallization method.
Crystal Structure of Urea Carboxylase Provides Insights into the Carboxyltransfer Reaction
Research from the Chinese Academy of Sciences could shed light on the mechanism of these enzymes' catalysis.
Vitamins could lead to the Design of Novel Drugs to Combat Malaria
The research will enable scientists to learn more about the nature of the enzymes required for vitamin biosynthesis by the malaria causing pathogen Plasmodium.
A New Generation of Crystallographic Validation Tools for the Protein Data Bank
This report presents the conclusions of the X-ray Validation Task Force of the worldwide Protein Data Bank.
Temperature Controlled Stage Used in Crystallisation Processes
Linkam Scientific Instrument report on the work of Professor Yves Henri Geerts from the Université Libre de Bruxelles where he uses a specially designed temperature stage to study crystallisation processes in opto-electronic thin films.
CSHL Team Solves a Protein Complex’s Molecular Structure to Explain Role in Gene Silencing
Scientists from Cold Spring Harbor Laboratory and their collaborators at St. Jude’s Research Hospital have discovered new details of how various domains of the protein complex contribute to heterochromatin assembly and gene silencing.
Noncovalent Polymerization of Mesogens Crystallizes Lysozyme: Correlation Between Nonamphiphilic Lyotropic Liquid Crystal Phase and Protein Crystal Formation
Researchers from Syracuse University we report the use of organic salts that can form a class of unusual nonamphiphilic lyotropic liquid crystals to crystallize the protein lysozyme.
Leading Proteomics Company Transitions to Emerald Bio, Chooses Key Leadership to Address Growing Market Opportunities
Emerald BioSystems will now be known as Emerald Bio. The new management changes include the appointment of CEO, George Abe, and the promotion of Dr. Peter Nollert to Chief Technologist.
Proteros Appoints Dr Klaus Hinterding
Dr Klaus Hinterding, formerly at Hoffmann-La Roche Ltd, joins Proteros biostructures GmbH as Chief Scientific Officer and member of the management board.
Nancy Hutson Appointed to BioCryst’s Board of Directors
Dr Hutson brings over 30 years of experience as a seasoned professional and leader within the pharmaceutical industry.
Proteros Obtains CIR Accreditation
Accreditation allows eligible clients to receive 30% of their R&D costs in tax credits.
Positive Results from Phase 2B Study into Patients with Gout
BioCryst Pharmaceuticals, Inc. announce results from its randomized Phase 2b study of BCX4208 added to allopurinol in patients with gout who had failed to reach the serum uric acid therapeutic goal of <6 mg/dL on allopurinol alone.
FEI Collaborates with NIH to Create New ‘Living Lab’ for Structural Biology Research
Collaboration combines electron microscopy, NMR and X-ray diffraction methods to provide advances in structural biology using cryo-EM to accelerate discoveries about the causes, treatments and cures for disease.
TTP LabTech Presents Protein Crystallography Community with News Service
TTP LabTech has launched the 2011 issue of its LabCrystal magazine, designed specifically for scientists who perform protein crystallography.
Bruker and Berkeley Lab Collaborate on Novel Technology for Structural Biology
Berkeley Lab and Bruker intend to integrate SAXS and NMR data for 3D structure determination of multi-domain proteins and protein complexes with DNA, RNA or other proteins.
Mitegen Announces Mutual Distribution Agreements with AJK Analytical Services
AJK to promote and sell Mitegens product lines in the Netherlands and other European countries.
Mitegen Announces Distribution Agreement with Helix Biotech in Malaysia
Under the agreement, Helix Biotech will promote and sell Mitegens MicroMount™, MicroLoop™, MicroMesh™, RT Aligner™ and other product lines in Malaysia.
Emerald BioSystems will now be known as Emerald Bio. The new management changes include the appointment of CEO, George Abe, and the promotion of Dr. Peter Nollert to Chief Technologist.
Proteros Appoints Dr Klaus Hinterding
Dr Klaus Hinterding, formerly at Hoffmann-La Roche Ltd, joins Proteros biostructures GmbH as Chief Scientific Officer and member of the management board.
Nancy Hutson Appointed to BioCryst’s Board of Directors
Dr Hutson brings over 30 years of experience as a seasoned professional and leader within the pharmaceutical industry.
Proteros Obtains CIR Accreditation
Accreditation allows eligible clients to receive 30% of their R&D costs in tax credits.
Positive Results from Phase 2B Study into Patients with Gout
BioCryst Pharmaceuticals, Inc. announce results from its randomized Phase 2b study of BCX4208 added to allopurinol in patients with gout who had failed to reach the serum uric acid therapeutic goal of <6 mg/dL on allopurinol alone.
FEI Collaborates with NIH to Create New ‘Living Lab’ for Structural Biology Research
Collaboration combines electron microscopy, NMR and X-ray diffraction methods to provide advances in structural biology using cryo-EM to accelerate discoveries about the causes, treatments and cures for disease.
TTP LabTech Presents Protein Crystallography Community with News Service
TTP LabTech has launched the 2011 issue of its LabCrystal magazine, designed specifically for scientists who perform protein crystallography.
Bruker and Berkeley Lab Collaborate on Novel Technology for Structural Biology
Berkeley Lab and Bruker intend to integrate SAXS and NMR data for 3D structure determination of multi-domain proteins and protein complexes with DNA, RNA or other proteins.
Mitegen Announces Mutual Distribution Agreements with AJK Analytical Services
AJK to promote and sell Mitegens product lines in the Netherlands and other European countries.
Mitegen Announces Distribution Agreement with Helix Biotech in Malaysia
Under the agreement, Helix Biotech will promote and sell Mitegens MicroMount™, MicroLoop™, MicroMesh™, RT Aligner™ and other product lines in Malaysia.
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A fast and fully automated solution for Lipidic Cubic Phase (LCP) screening using mosquito LCP
1TTP Labtech Ltd 2MRC
Membrane proteins, such as G-protein-coupled receptors, are known to be much more difficult to purify and crystallise than soluble proteins due to their native environment within the lipid bilayer of the cell membrane. The in meso (lipidic cubic phase or LCP) crystallisation technique has revolutionised the process of crystallising membrane proteins. This method utilises highly viscous lipid mesophases to contain the membrane proteins for crystallisation.
mosquito® Crystal: Fast, reliable automation of Protein Crystallization drop set-up
TTP Labtech Ltd
Automation of protein crystallography screening has contributed significantly to the rapid progress of crystallography based structural biology. Automation allows samples to be screened using smaller volumes of both protein and screen solutions, reducing costs and saving valuable protein. Additional benefits include increased throughput and accuracy. One of the challenges to automating this process is the necessity to accurately pipette solutions of varying viscosities
Analysis of overall success of robotic crystallization
Laboratory of Structural Biology, School of Biological Sciences
A total of about 140 different proteins have been commissioned and 62 have been crystallized with a quality suitable for X-ray diffraction (45%). On a per-drop basis, 300 crystallization experiments have been successful, representing an over¬all success rate of 3%. PEG 3350 was the precipitant for 20, making it the single most successful crystallization agent.
Strategies for expression and solubility analysis
Production of soluble protein is one of the major bottlenecks that precede crystallographic studies.During the last years several techniques and strategies have been developed to address this problem. However, many of them imply an economical cost and technologies that are not always available. We will describe a general plan for protein solubility analysis by using a combination of four different but complementary strategies. In this plan, different constructs of a protein interest are designed
Automating Microseeding Protein Crystallography Set-ups Using Mosquito
TTP Labtech
Crystallising proteins, required for structure determination by X-ray diffraction, is a difficult and labour-intensive task. The mosquito liquid handler (TTP LabTech) is ideally suited to automating the complex set-ups required for microseeding due to its precide handling of extremely low volumes of even viscous solutions, and its ability to perform multiple aspirations and dispenses with each pipette.
Automated Single Crystal Structure Determination - A Tool for Synthetic Chemists?
Bruker AXS
During recent years large improvements in software functionality and its ease-of-use have made single crystal X-ray structure determination easier than ever. These days most structures can be measured, processed, solved and refined using well selected defaults with no or little crystallographic knowledge. Recently, microfocus sources and CCD detectors both air-cooled, have entered the marketplace. Combining these innovations with an automated sample loader and an intelligent graphical user inte
Advances in Crystallographic Hardware for Structural Biology
Bruker AXS
The number of life science investigators utilizing crystallography in their research increases every year. Synchrotrons are an invaluable resource for structural work but not everyone has unlimited access to a beamline. In-house diffraction systems complement synchrotron access and can provide increased productivity in the home lab. In recent years, the performance and versatility of in-house systems has greatly improved. The enhanced performance allows data collection on demanding projects such
MED-SuMo and MED-Hybridise: exploit all PDB macromolecule structures to design/optimize innovative leads
MEDIT
Obtaining structural information on fragment-protein target is a key factor and also a major limitation. We’ve used MED-SuMo and MED-Hybridise to query and mine the PDB seeking for similar interaction surfaces. MED-portions were used to design novel scaffolds (GPCR) and decorate a given scaffold (kinase). We have analysed the scaffolds in regard to their diversity, their presence in PubChem, PDB and other libraries.
Phase Diagram Visualization via Continuously-Fed Crystallization: Experiments and Model
University of Minnesota
A continuously-fed crystallization chamber is manufactured to allow phase diagram visualization. This microfluidic system allows the experimenter to screen a large range of salt and protein concentrations in one experiment. This allows the experimenter to predict the protein phase diagram in a single experiment. A continuous-feed crystallization chamber has been successfully fabricated and characterized in terms of its flow profile, and has successfully predicted the phase diagram for lysozyme.
Low Volume Liquid Handling of Organic Solvents for Compound Storage and Dissolution using mosquito
TTP Labtech
mosquito® is a positive displacement liquid handling system capable of pipetting solutions with a high degree of accuracy (to within 5%) and precision (CVs of <10%) in the nanolitre range. Here, we demonstrate mosquito’s ability to dispense accurately low volumes of organic solvents of low surface tension and to re-dissolve compounds which have been previously dried in storage wells.
1TTP Labtech Ltd 2MRC
Membrane proteins, such as G-protein-coupled receptors, are known to be much more difficult to purify and crystallise than soluble proteins due to their native environment within the lipid bilayer of the cell membrane. The in meso (lipidic cubic phase or LCP) crystallisation technique has revolutionised the process of crystallising membrane proteins. This method utilises highly viscous lipid mesophases to contain the membrane proteins for crystallisation.
mosquito® Crystal: Fast, reliable automation of Protein Crystallization drop set-up
TTP Labtech Ltd
Automation of protein crystallography screening has contributed significantly to the rapid progress of crystallography based structural biology. Automation allows samples to be screened using smaller volumes of both protein and screen solutions, reducing costs and saving valuable protein. Additional benefits include increased throughput and accuracy. One of the challenges to automating this process is the necessity to accurately pipette solutions of varying viscosities
Analysis of overall success of robotic crystallization
Laboratory of Structural Biology, School of Biological Sciences
A total of about 140 different proteins have been commissioned and 62 have been crystallized with a quality suitable for X-ray diffraction (45%). On a per-drop basis, 300 crystallization experiments have been successful, representing an over¬all success rate of 3%. PEG 3350 was the precipitant for 20, making it the single most successful crystallization agent.
Strategies for expression and solubility analysis
Production of soluble protein is one of the major bottlenecks that precede crystallographic studies.During the last years several techniques and strategies have been developed to address this problem. However, many of them imply an economical cost and technologies that are not always available. We will describe a general plan for protein solubility analysis by using a combination of four different but complementary strategies. In this plan, different constructs of a protein interest are designed
Automating Microseeding Protein Crystallography Set-ups Using Mosquito
TTP Labtech
Crystallising proteins, required for structure determination by X-ray diffraction, is a difficult and labour-intensive task. The mosquito liquid handler (TTP LabTech) is ideally suited to automating the complex set-ups required for microseeding due to its precide handling of extremely low volumes of even viscous solutions, and its ability to perform multiple aspirations and dispenses with each pipette.
Automated Single Crystal Structure Determination - A Tool for Synthetic Chemists?
Bruker AXS
During recent years large improvements in software functionality and its ease-of-use have made single crystal X-ray structure determination easier than ever. These days most structures can be measured, processed, solved and refined using well selected defaults with no or little crystallographic knowledge. Recently, microfocus sources and CCD detectors both air-cooled, have entered the marketplace. Combining these innovations with an automated sample loader and an intelligent graphical user inte
Advances in Crystallographic Hardware for Structural Biology
Bruker AXS
The number of life science investigators utilizing crystallography in their research increases every year. Synchrotrons are an invaluable resource for structural work but not everyone has unlimited access to a beamline. In-house diffraction systems complement synchrotron access and can provide increased productivity in the home lab. In recent years, the performance and versatility of in-house systems has greatly improved. The enhanced performance allows data collection on demanding projects such
MED-SuMo and MED-Hybridise: exploit all PDB macromolecule structures to design/optimize innovative leads
MEDIT
Obtaining structural information on fragment-protein target is a key factor and also a major limitation. We’ve used MED-SuMo and MED-Hybridise to query and mine the PDB seeking for similar interaction surfaces. MED-portions were used to design novel scaffolds (GPCR) and decorate a given scaffold (kinase). We have analysed the scaffolds in regard to their diversity, their presence in PubChem, PDB and other libraries.
Phase Diagram Visualization via Continuously-Fed Crystallization: Experiments and Model
University of Minnesota
A continuously-fed crystallization chamber is manufactured to allow phase diagram visualization. This microfluidic system allows the experimenter to screen a large range of salt and protein concentrations in one experiment. This allows the experimenter to predict the protein phase diagram in a single experiment. A continuous-feed crystallization chamber has been successfully fabricated and characterized in terms of its flow profile, and has successfully predicted the phase diagram for lysozyme.
Low Volume Liquid Handling of Organic Solvents for Compound Storage and Dissolution using mosquito
TTP Labtech
mosquito® is a positive displacement liquid handling system capable of pipetting solutions with a high degree of accuracy (to within 5%) and precision (CVs of <10%) in the nanolitre range. Here, we demonstrate mosquito’s ability to dispense accurately low volumes of organic solvents of low surface tension and to re-dissolve compounds which have been previously dried in storage wells.
mosquito® Crystal for the rapid screening and optimisation of crystallisation conditions of the bacterial periplasmic binding protein, HbpA
TTP LabTech
Glutathione is one of the major antioxidants/redox molecules in most aerobic cells, playing an important role in cell metabolism, differentiation, proliferation, and apoptosis. The reduced form of glutathione (GSH) is required for the detoxification of drugs and reactive compounds of both endogenous and exogenous origin.
Heating on an Atlas Hotplate
Syrris Ltd
In the following experiments, different vessels were heated using the Atlas hotplate, with and without the Atlas Stacking Drybath. In every case, an external thermocouple probe has been used with the system to accurately measure “reaction” temperature.
Analysing Endotherms using Atlas
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at endothermic processes.
Tracking Exotherms using Atlas Reaction Monitoring
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at exothermic processes.
Reaction Monitoring with Atlas 1
Syrris Ltd
This application note reports the use of the atlas system to monitor the hydrolysis of acetic anhydride, a typical test reaction for exothermic processes.
Reaction Monitoring with Atlas 2
Syrris Ltd
This application note details an experiment to show the repeatability and scalability of reaction monitoring signals by using an electrical heater system.
Examples of FRX Applications for Demonstration
Syrris Ltd
FRX as a product offers a wide range of applications in a number of areas. To try and increase the exposure of the product to a wider audience, two standard demonstrations are detailed here. The first is a Wittig reaction showing the operation of an FRX200 system. The second is a separation of water and ethyl acetate demonstrating the operation of the FLLEX (Flow Liquid Liquid EXtraction module), for FRX300 or FRX400.
Cleaning Microreactors
Syrris Ltd
This application note gives hints and tips on cleaning & unblocking flow reactors, i.e. chips or tube based reactors. It is suggested that the whole document is read before attempting to unblock a reactor.
The Basics of Flow Chemistry
Syrris Ltd
This application note gives information on the basics of flow chemistry, with particular reference to the FRX system.
Planning Successful Reactions
Syrris Ltd
This application note gives hints and tips for planning successful FRX reactions.
TTP LabTech
Glutathione is one of the major antioxidants/redox molecules in most aerobic cells, playing an important role in cell metabolism, differentiation, proliferation, and apoptosis. The reduced form of glutathione (GSH) is required for the detoxification of drugs and reactive compounds of both endogenous and exogenous origin.
Heating on an Atlas Hotplate
Syrris Ltd
In the following experiments, different vessels were heated using the Atlas hotplate, with and without the Atlas Stacking Drybath. In every case, an external thermocouple probe has been used with the system to accurately measure “reaction” temperature.
Analysing Endotherms using Atlas
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at endothermic processes.
Tracking Exotherms using Atlas Reaction Monitoring
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at exothermic processes.
Reaction Monitoring with Atlas 1
Syrris Ltd
This application note reports the use of the atlas system to monitor the hydrolysis of acetic anhydride, a typical test reaction for exothermic processes.
Reaction Monitoring with Atlas 2
Syrris Ltd
This application note details an experiment to show the repeatability and scalability of reaction monitoring signals by using an electrical heater system.
Examples of FRX Applications for Demonstration
Syrris Ltd
FRX as a product offers a wide range of applications in a number of areas. To try and increase the exposure of the product to a wider audience, two standard demonstrations are detailed here. The first is a Wittig reaction showing the operation of an FRX200 system. The second is a separation of water and ethyl acetate demonstrating the operation of the FLLEX (Flow Liquid Liquid EXtraction module), for FRX300 or FRX400.
Cleaning Microreactors
Syrris Ltd
This application note gives hints and tips on cleaning & unblocking flow reactors, i.e. chips or tube based reactors. It is suggested that the whole document is read before attempting to unblock a reactor.
The Basics of Flow Chemistry
Syrris Ltd
This application note gives information on the basics of flow chemistry, with particular reference to the FRX system.
Planning Successful Reactions
Syrris Ltd
This application note gives hints and tips for planning successful FRX reactions.
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