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 | Chemical Biology Approaches to Target Protein Modification in Disease Megan Wright, Imperial College London, speaking at Discovery Chemistry Congress 2013. |
 | Robust Three-dimensional Tools to Design Inhibitors Against Drug Targets Which Lack Explicit Specificity Dr. Moses Mohandas Prabu, The Commonwealth Medical College, speaking at Discovery Chemistry Congress 2013. |
Lab Instrument Rental Trends 2012
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on lab instrument rental carried out in September 2012.
Novel Antibodies & Therapeutic Proteins: Technological Challenges & Future Prospects
Select Biosciences Ltd
This innovative & analytical report can be used to assess market trends & appraise the potential of new antibodies & therapeutic proteins. It provides perspective to gain an in-depth understanding of the marketplace, players, products & leading technologies driving the field.
Molecular Diagnostics Market: Trends and Opportunities
Koncept Analytics
Molecular diagnostics or the analysis of DNA and RNA at the molecular level - is a fast-growing market, driven by the growing understanding of the human genome. Although a small part of the In-Vitro Diagnostics (IVD) market, molecular diagnostics market is one of the two growth drivers of the IVD industry. In the decision making process of healthcare experts, diagnostics tests have a major influence.
Multiplexed Diagnostics 2010
Select Biosciences Ltd
The multiplexed diagnostics market has grown rapidly and generated sales of approximately $2.4 billon in 2009, but is now balanced on multiple tipping points.
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on lab instrument rental carried out in September 2012.
Novel Antibodies & Therapeutic Proteins: Technological Challenges & Future Prospects
Select Biosciences Ltd
This innovative & analytical report can be used to assess market trends & appraise the potential of new antibodies & therapeutic proteins. It provides perspective to gain an in-depth understanding of the marketplace, players, products & leading technologies driving the field.
Molecular Diagnostics Market: Trends and Opportunities
Koncept Analytics
Molecular diagnostics or the analysis of DNA and RNA at the molecular level - is a fast-growing market, driven by the growing understanding of the human genome. Although a small part of the In-Vitro Diagnostics (IVD) market, molecular diagnostics market is one of the two growth drivers of the IVD industry. In the decision making process of healthcare experts, diagnostics tests have a major influence.
Multiplexed Diagnostics 2010
Select Biosciences Ltd
The multiplexed diagnostics market has grown rapidly and generated sales of approximately $2.4 billon in 2009, but is now balanced on multiple tipping points.
Using X-rays to Study Ultra-Thin Transistors
Cornell scientists have helped develop a novel process of spreading extremely thin organic transistors, and used synchrotron X-rays to watch how the films crystallize.
Crystal Structure of the Capsular Polysaccharide Synthesizing Protein CapE of Staphylococcus Aureus
This Study presents the first crystal structure of CapE, revealing that this enzyme forms a stable and functional homo-hexamer.
Proton Pathway in Photosynthesis Identified by X-ray Crystallography
A Purdue University-led team has revealed the proton transfer pathway responsible for a majority of energy storage in photosynthesis.
The Cation-π Box is a Specific Phosphatidylcholine Membrane Targeting Motif
Well-defined choline cationaromatic p interactions may be a general motif to anchor proteins to PC-rich bilayers.
Crystallography on the Nanogram Scale
Single Crystal X-ray analysis is a powerful tool for determining the structures of molecules and is widely used both in academic and industrial research.
Proteros and Priaxon Announce Lead Discovery Collaboration on Protein-Protein Interaction Targets
Proteros biostructures GmbH announced they entered into a Lead Discovery Collaboration with Priaxon AG to jointly develop lead compounds for high value protein-protein interaction (PPI) drug targets.
Winners of the Aminoff Prize in Crystallography Announced
Carlo Gatti and Mark Spackman developed experimental and theoretical methods to study electron density in crystals, and using them to determine molecular and crystalline properties.
In Silico Prediction of Interactions between Site II on Human Serum Albumin and Profen Drugs
Binding of a drug molecule to HSA results in increased solubility in plasma, decreased toxicity, and protection against oxidation of the bound molecule.
Study Reveals How Serotonin Receptors Can Shape Drug Effects from LSD to Migraine Medication
A team of scientists has determined and analyzed the high-resolution atomic structures of two kinds of human serotonin receptor.
Findings to Help in Design of Drugs against Virus Causing Childhood Illnesses
New research findings may help scientists design drugs to treat a virus infection that causes potentially fatal brain swelling and paralysis in children.
Cornell scientists have helped develop a novel process of spreading extremely thin organic transistors, and used synchrotron X-rays to watch how the films crystallize.
Crystal Structure of the Capsular Polysaccharide Synthesizing Protein CapE of Staphylococcus Aureus
This Study presents the first crystal structure of CapE, revealing that this enzyme forms a stable and functional homo-hexamer.
Proton Pathway in Photosynthesis Identified by X-ray Crystallography
A Purdue University-led team has revealed the proton transfer pathway responsible for a majority of energy storage in photosynthesis.
The Cation-π Box is a Specific Phosphatidylcholine Membrane Targeting Motif
Well-defined choline cationaromatic p interactions may be a general motif to anchor proteins to PC-rich bilayers.
Crystallography on the Nanogram Scale
Single Crystal X-ray analysis is a powerful tool for determining the structures of molecules and is widely used both in academic and industrial research.
Proteros and Priaxon Announce Lead Discovery Collaboration on Protein-Protein Interaction Targets
Proteros biostructures GmbH announced they entered into a Lead Discovery Collaboration with Priaxon AG to jointly develop lead compounds for high value protein-protein interaction (PPI) drug targets.
Winners of the Aminoff Prize in Crystallography Announced
Carlo Gatti and Mark Spackman developed experimental and theoretical methods to study electron density in crystals, and using them to determine molecular and crystalline properties.
In Silico Prediction of Interactions between Site II on Human Serum Albumin and Profen Drugs
Binding of a drug molecule to HSA results in increased solubility in plasma, decreased toxicity, and protection against oxidation of the bound molecule.
Study Reveals How Serotonin Receptors Can Shape Drug Effects from LSD to Migraine Medication
A team of scientists has determined and analyzed the high-resolution atomic structures of two kinds of human serotonin receptor.
Findings to Help in Design of Drugs against Virus Causing Childhood Illnesses
New research findings may help scientists design drugs to treat a virus infection that causes potentially fatal brain swelling and paralysis in children.
Evotec and Harvard University to Collaborate on Development of New Class of Antibacterials
Target PGB (peptidoglycan biosynthesis) is the focus of collaboration.
Proteros And Eisai Achieve Target in Lead Discovery Project
Companies delivering kinetically optimized lead compounds.
CNR Investigates Biodegradable and Biocompatible Polymer Research
Linkam Scientific Instruments report on the use of their THMS600 stage for polymer research at the Institute of Chemistry and Technology of Polymers (CNR), Italy.
A New EU-Funded Industry-Academia Drug Discovery Partnership Targets Challenging Kinases
Industry and academia join to create the Translational Kinase Tumor Inhibitor Discovery Consortium.
Bruker Opens Two New Centers of Excellence in Mumbai and Bengaluru
The applications, training and service centers will offer extended applications laboratories and customer support capabilities.
Crystal Pharmatech and Particle Sciences Form CRO Partnership
Two companies bring intelligent, optimal solutions for insoluble drugs to innovative pharmaceutical companies.
Lyncean Technologies Inc. Sells Compact Light Source to Munich Biomedical-Imaging Research Center
Palo Alto-based Lyncean Technologies, Inc., announced its first sale of a Compact Light Source, a miniature synchrotron X-ray source employing state-of-the-art laser-beam and electron-beam technology.
Peter Rehse joins BioFocus as Senior Director, Structural Biology
BioFocus today announced that Dr. Peter Rehse has joined the company and will be based at Chesterford Park, near Cambridge, UK.
NovAliX Partners with Kyowa Hakko Kirin
NovAliX SAS announced today that it has entered into a fragment-based drug discovery alliance with Kyowa Hakko Kirin Co., Ltd., Japan.
Agilent Technologies Opens New X-ray Diffraction Manufacturing Facility
The state-of-the-art facility in Wroclaw, Poland gives Agilent significantly greater manufacturing capacity and enhanced R&D capabilities compared with the company's old facility.
Target PGB (peptidoglycan biosynthesis) is the focus of collaboration.
Proteros And Eisai Achieve Target in Lead Discovery Project
Companies delivering kinetically optimized lead compounds.
CNR Investigates Biodegradable and Biocompatible Polymer Research
Linkam Scientific Instruments report on the use of their THMS600 stage for polymer research at the Institute of Chemistry and Technology of Polymers (CNR), Italy.
A New EU-Funded Industry-Academia Drug Discovery Partnership Targets Challenging Kinases
Industry and academia join to create the Translational Kinase Tumor Inhibitor Discovery Consortium.
Bruker Opens Two New Centers of Excellence in Mumbai and Bengaluru
The applications, training and service centers will offer extended applications laboratories and customer support capabilities.
Crystal Pharmatech and Particle Sciences Form CRO Partnership
Two companies bring intelligent, optimal solutions for insoluble drugs to innovative pharmaceutical companies.
Lyncean Technologies Inc. Sells Compact Light Source to Munich Biomedical-Imaging Research Center
Palo Alto-based Lyncean Technologies, Inc., announced its first sale of a Compact Light Source, a miniature synchrotron X-ray source employing state-of-the-art laser-beam and electron-beam technology.
Peter Rehse joins BioFocus as Senior Director, Structural Biology
BioFocus today announced that Dr. Peter Rehse has joined the company and will be based at Chesterford Park, near Cambridge, UK.
NovAliX Partners with Kyowa Hakko Kirin
NovAliX SAS announced today that it has entered into a fragment-based drug discovery alliance with Kyowa Hakko Kirin Co., Ltd., Japan.
Agilent Technologies Opens New X-ray Diffraction Manufacturing Facility
The state-of-the-art facility in Wroclaw, Poland gives Agilent significantly greater manufacturing capacity and enhanced R&D capabilities compared with the company's old facility.
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Structure determination of the virulence factor AsaP1 from Aeromonas salmonicida subsp. Achromogenes
CyBio
Aeromonas commonly found in waters, wastewaters and mud are non-motile gram negative bacteria. Atypical strains of Aeromonas salmonicida are known to be largely responsible for causing a lethal disease in Atlantic salmons and other farmed fishes.
Stable N-phosphorylated triazol-5-ylidenes
National Academy of Sciences of Ukraine
Considering probable generality of “carbene” mechanism for 1,3-azoles, investigation of intermediates of phosphorylation of 1,2,4-triazoles is of interest.
A Crystallographic-Based Fragment Screen Against Human BRD4 Bromodomain 1
BioFocus
Fragment-based drug discovery has now become firmly established as an important method in the lead discovery process, and is an integral component of drug discovery at BioFocus. Using a selection of compounds from our fragment collection, we have performed a crystallographic-based screen against human BRD4 bromodomain 1.
A fast and fully automated solution for Lipidic Cubic Phase (LCP) screening using mosquito LCP
1TTP Labtech Ltd 2MRC
Membrane proteins, such as G-protein-coupled receptors, are known to be much more difficult to purify and crystallise than soluble proteins due to their native environment within the lipid bilayer of the cell membrane. The in meso (lipidic cubic phase or LCP) crystallisation technique has revolutionised the process of crystallising membrane proteins. This method utilises highly viscous lipid mesophases to contain the membrane proteins for crystallisation.
mosquito® Crystal: Fast, reliable automation of Protein Crystallization drop set-up
TTP Labtech Ltd
Automation of protein crystallography screening has contributed significantly to the rapid progress of crystallography based structural biology. Automation allows samples to be screened using smaller volumes of both protein and screen solutions, reducing costs and saving valuable protein. Additional benefits include increased throughput and accuracy. One of the challenges to automating this process is the necessity to accurately pipette solutions of varying viscosities
Analysis of overall success of robotic crystallization
Laboratory of Structural Biology, School of Biological Sciences
A total of about 140 different proteins have been commissioned and 62 have been crystallized with a quality suitable for X-ray diffraction (45%). On a per-drop basis, 300 crystallization experiments have been successful, representing an over¬all success rate of 3%. PEG 3350 was the precipitant for 20, making it the single most successful crystallization agent.
Strategies for expression and solubility analysis
Production of soluble protein is one of the major bottlenecks that precede crystallographic studies.During the last years several techniques and strategies have been developed to address this problem. However, many of them imply an economical cost and technologies that are not always available. We will describe a general plan for protein solubility analysis by using a combination of four different but complementary strategies. In this plan, different constructs of a protein interest are designed
Automating Microseeding Protein Crystallography Set-ups Using Mosquito
TTP Labtech
Crystallising proteins, required for structure determination by X-ray diffraction, is a difficult and labour-intensive task. The mosquito liquid handler (TTP LabTech) is ideally suited to automating the complex set-ups required for microseeding due to its precide handling of extremely low volumes of even viscous solutions, and its ability to perform multiple aspirations and dispenses with each pipette.
Automated Single Crystal Structure Determination - A Tool for Synthetic Chemists?
Bruker AXS
During recent years large improvements in software functionality and its ease-of-use have made single crystal X-ray structure determination easier than ever. These days most structures can be measured, processed, solved and refined using well selected defaults with no or little crystallographic knowledge. Recently, microfocus sources and CCD detectors both air-cooled, have entered the marketplace. Combining these innovations with an automated sample loader and an intelligent graphical user inte
Advances in Crystallographic Hardware for Structural Biology
Bruker AXS
The number of life science investigators utilizing crystallography in their research increases every year. Synchrotrons are an invaluable resource for structural work but not everyone has unlimited access to a beamline. In-house diffraction systems complement synchrotron access and can provide increased productivity in the home lab. In recent years, the performance and versatility of in-house systems has greatly improved. The enhanced performance allows data collection on demanding projects such
CyBio
Aeromonas commonly found in waters, wastewaters and mud are non-motile gram negative bacteria. Atypical strains of Aeromonas salmonicida are known to be largely responsible for causing a lethal disease in Atlantic salmons and other farmed fishes.
Stable N-phosphorylated triazol-5-ylidenes
National Academy of Sciences of Ukraine
Considering probable generality of “carbene” mechanism for 1,3-azoles, investigation of intermediates of phosphorylation of 1,2,4-triazoles is of interest.
A Crystallographic-Based Fragment Screen Against Human BRD4 Bromodomain 1
BioFocus
Fragment-based drug discovery has now become firmly established as an important method in the lead discovery process, and is an integral component of drug discovery at BioFocus. Using a selection of compounds from our fragment collection, we have performed a crystallographic-based screen against human BRD4 bromodomain 1.
A fast and fully automated solution for Lipidic Cubic Phase (LCP) screening using mosquito LCP
1TTP Labtech Ltd 2MRC
Membrane proteins, such as G-protein-coupled receptors, are known to be much more difficult to purify and crystallise than soluble proteins due to their native environment within the lipid bilayer of the cell membrane. The in meso (lipidic cubic phase or LCP) crystallisation technique has revolutionised the process of crystallising membrane proteins. This method utilises highly viscous lipid mesophases to contain the membrane proteins for crystallisation.
mosquito® Crystal: Fast, reliable automation of Protein Crystallization drop set-up
TTP Labtech Ltd
Automation of protein crystallography screening has contributed significantly to the rapid progress of crystallography based structural biology. Automation allows samples to be screened using smaller volumes of both protein and screen solutions, reducing costs and saving valuable protein. Additional benefits include increased throughput and accuracy. One of the challenges to automating this process is the necessity to accurately pipette solutions of varying viscosities
Analysis of overall success of robotic crystallization
Laboratory of Structural Biology, School of Biological Sciences
A total of about 140 different proteins have been commissioned and 62 have been crystallized with a quality suitable for X-ray diffraction (45%). On a per-drop basis, 300 crystallization experiments have been successful, representing an over¬all success rate of 3%. PEG 3350 was the precipitant for 20, making it the single most successful crystallization agent.
Strategies for expression and solubility analysis
Production of soluble protein is one of the major bottlenecks that precede crystallographic studies.During the last years several techniques and strategies have been developed to address this problem. However, many of them imply an economical cost and technologies that are not always available. We will describe a general plan for protein solubility analysis by using a combination of four different but complementary strategies. In this plan, different constructs of a protein interest are designed
Automating Microseeding Protein Crystallography Set-ups Using Mosquito
TTP Labtech
Crystallising proteins, required for structure determination by X-ray diffraction, is a difficult and labour-intensive task. The mosquito liquid handler (TTP LabTech) is ideally suited to automating the complex set-ups required for microseeding due to its precide handling of extremely low volumes of even viscous solutions, and its ability to perform multiple aspirations and dispenses with each pipette.
Automated Single Crystal Structure Determination - A Tool for Synthetic Chemists?
Bruker AXS
During recent years large improvements in software functionality and its ease-of-use have made single crystal X-ray structure determination easier than ever. These days most structures can be measured, processed, solved and refined using well selected defaults with no or little crystallographic knowledge. Recently, microfocus sources and CCD detectors both air-cooled, have entered the marketplace. Combining these innovations with an automated sample loader and an intelligent graphical user inte
Advances in Crystallographic Hardware for Structural Biology
Bruker AXS
The number of life science investigators utilizing crystallography in their research increases every year. Synchrotrons are an invaluable resource for structural work but not everyone has unlimited access to a beamline. In-house diffraction systems complement synchrotron access and can provide increased productivity in the home lab. In recent years, the performance and versatility of in-house systems has greatly improved. The enhanced performance allows data collection on demanding projects such
Fully automated sitting drop protein crystallization with CyBi®-CrystalCreator using JBScreen Basic HTS Kit
CyBio
Growing high-quality protein crystals is still a major bottleneck and very time consuming step in this process. This application note describes screening experiments using the fully automated CyBi®-CrystalCreator in combination with a commercially available crystallization screen from Jena Bioscience.
Nanoliter Scale High-Throughput Protein Crystallography Screening with the Echo Liquid Handler
Labcyte
The Echo® liquid handler enables protein crystal formation with sitting drop volumes as small as 50 nL. Nanoliter volumes of protein and crystallography reagents can be transferred into sitting drop wells of crystallography plates. Precise and accurate drop placement eliminates cross-contamination and ensures drop-on-drop placement for blending protein and crystallography solutions.
Precision of the 96-Channel Heads of the CyBi-CrystalCreator
CyBio
This application note shows precision data in the low volume range of the 96-channel head of the CyBi-CrystalCreator using different volumes and pipetting modes. The precision was determined using a standardized absorption method.
Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias
CyBio
Using this technique, it was found that while buffer components alone without seed can marginally promote crystal growth in some cases, crystal seeding is far more effective in boosting the number of sparsematrix conditions that yield protein crystals.
mosquito® Crystal for the rapid screening and optimisation of crystallisation conditions of the bacterial periplasmic binding protein, HbpA
TTP LabTech
Glutathione is one of the major antioxidants/redox molecules in most aerobic cells, playing an important role in cell metabolism, differentiation, proliferation, and apoptosis. The reduced form of glutathione (GSH) is required for the detoxification of drugs and reactive compounds of both endogenous and exogenous origin.
Heating on an Atlas Hotplate
Syrris Ltd
In the following experiments, different vessels were heated using the Atlas hotplate, with and without the Atlas Stacking Drybath. In every case, an external thermocouple probe has been used with the system to accurately measure “reaction” temperature.
Analysing Endotherms using Atlas
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at endothermic processes.
Tracking Exotherms using Atlas Reaction Monitoring
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at exothermic processes.
Reaction Monitoring with Atlas 1
Syrris Ltd
This application note reports the use of the atlas system to monitor the hydrolysis of acetic anhydride, a typical test reaction for exothermic processes.
Reaction Monitoring with Atlas 2
Syrris Ltd
This application note details an experiment to show the repeatability and scalability of reaction monitoring signals by using an electrical heater system.
CyBio
Growing high-quality protein crystals is still a major bottleneck and very time consuming step in this process. This application note describes screening experiments using the fully automated CyBi®-CrystalCreator in combination with a commercially available crystallization screen from Jena Bioscience.
Nanoliter Scale High-Throughput Protein Crystallography Screening with the Echo Liquid Handler
Labcyte
The Echo® liquid handler enables protein crystal formation with sitting drop volumes as small as 50 nL. Nanoliter volumes of protein and crystallography reagents can be transferred into sitting drop wells of crystallography plates. Precise and accurate drop placement eliminates cross-contamination and ensures drop-on-drop placement for blending protein and crystallography solutions.
Precision of the 96-Channel Heads of the CyBi-CrystalCreator
CyBio
This application note shows precision data in the low volume range of the 96-channel head of the CyBi-CrystalCreator using different volumes and pipetting modes. The precision was determined using a standardized absorption method.
Acoustic matrix microseeding: improving protein crystal growth with minimal chemical bias
CyBio
Using this technique, it was found that while buffer components alone without seed can marginally promote crystal growth in some cases, crystal seeding is far more effective in boosting the number of sparsematrix conditions that yield protein crystals.
mosquito® Crystal for the rapid screening and optimisation of crystallisation conditions of the bacterial periplasmic binding protein, HbpA
TTP LabTech
Glutathione is one of the major antioxidants/redox molecules in most aerobic cells, playing an important role in cell metabolism, differentiation, proliferation, and apoptosis. The reduced form of glutathione (GSH) is required for the detoxification of drugs and reactive compounds of both endogenous and exogenous origin.
Heating on an Atlas Hotplate
Syrris Ltd
In the following experiments, different vessels were heated using the Atlas hotplate, with and without the Atlas Stacking Drybath. In every case, an external thermocouple probe has been used with the system to accurately measure “reaction” temperature.
Analysing Endotherms using Atlas
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at endothermic processes.
Tracking Exotherms using Atlas Reaction Monitoring
Syrris Ltd
This application note shows how atlas and reaction monitoring can be used to look at exothermic processes.
Reaction Monitoring with Atlas 1
Syrris Ltd
This application note reports the use of the atlas system to monitor the hydrolysis of acetic anhydride, a typical test reaction for exothermic processes.
Reaction Monitoring with Atlas 2
Syrris Ltd
This application note details an experiment to show the repeatability and scalability of reaction monitoring signals by using an electrical heater system.
