Corporate Banner
Satellite Banner
Crystallography
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Crystal Structure of a Human IaB Kinase ß Asymmetric Dimer

Published: Thursday, June 27, 2013
Last Updated: Thursday, June 27, 2013
Bookmark and Share
The observed domain movements in the structures of IKK may represent transphosphorylation steps that accompany IKK activation.

SUMMARY

Phosphorylation of inhibitor of nuclear  transcription factor B (IB) by IB  kinase (IKK) triggers the degradation of  IB and migration of cytoplasmic B to  the nucleus where it promotes the  transcription of its target genes.  Activation of IKK is achieved by phosphorylation of its main subunit,  IKK at the activation loop sites. Here  we report the 2.8 Å resolution crystal  structure of human IKKhIKK which  is partially phosphorylated and bound to the staurosporine analog K252a. The  hIKK protomer adopts a trimodular  structure that closely resembles that from  Xenopus laevis (xIKK: an N-terminal  kinase domain (KD), a central ubiquitinlike domain (ULD), and a C-terminal  dimerization domain (SDD). Although  hIKK and xIKK utilize a similar  dimerization mode, their overall  geometries are distinct. In contrast to the  structure resembling closed shears  reported previously for xIKK hIKK exists as an open asymmetric dimer in  which the two KDs are further apart,  with one in an active and the other in an  inactive conformation. Dimer  interactions are limited to the C-terminal  six-helix bundle that acts as a hinge  between the two subunits. The observed  domain movements in the structures of  IKK may represent transphosphorylation steps that accompany  IKK activation.

This article was published online in The Journal of Biological Chemistry and is free to access.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,100+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

X-Ray Free Electron Laser for Fixed-Target Protein Serial Microcrystallography
The article presents the first proof-of-principle results from fixed-target serial crystallography using the Linac Coherent Light Source.
Monday, October 06, 2014
Identifying, Studying and Making Good Use of Macromolecular Crystals
Techniques that extend the observation of crystals beyond the optical microscope will enhance structural biology in the future.
Monday, October 06, 2014
Mosquitoes Resistant to DDT Also Resistant to Mosquito Net Insecticide
Understanding how resistance works will help to develop tests, stopping resistance genes from spreading amongst mosquito populations.
Thursday, February 27, 2014
Defining the Communication Between Agonist and Coactivator Binding in the Retinoid X Receptor a Ligand Binding Domain
The four structural changes are conserved in all x-ray structures of the RXR ligand binding domain in the presence of agonist and coactivator peptide.
Wednesday, November 06, 2013
The Structure of Classical Swine Fever Virus Npro
This study reports the crystal structures of CSFV Npro and a C168A cleavage site Npro mutant to 1.6 Å resolution.
Friday, October 25, 2013
EphA5 Ligand Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations
This study reveals that the EphA5 LBD has dynamic properties very different from those of the EphA4 LBD, despite the 74% sequence identity and structural similarity.
Monday, October 07, 2013
3.5Å cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein
Hepatitis B virus (HBV) infection results in over a million deaths worldwide each year.
Tuesday, September 24, 2013
Two Pfam Protein Families Characterized by a Crystal Structure of Protein lpg2210
NIH centers are systematically targeting uncharacterized proteins with the goal of providing structural information for a significant portion of the protein universe.
Monday, September 09, 2013
Structural Insights into the Functions of the FANCM-FAAP24 Complex in DNA Repair
This study reports the crystal structure of human FANCM-FAAP24 complex, which together with the biological data provide the molecular insights into its functions in DNA repair.
Monday, September 09, 2013
Structural Insights into Central Hypertension Regulation by Human Aminopeptidase A
This study determined the crystal structures of APA complexed with different ligands and calcium.
Wednesday, July 31, 2013
The Serine Protease Domain of MASP-3: Enzymatic Properties and Crystal Structure in Complex with Ecotin
A tetrameric complex comprising an ecotin dimer and two MASP-3 SP domains was isolated and its crystal structure was solved.
Monday, July 22, 2013
Crystal Structure of a GroEL-ADP Complex in the Relaxed Allosteric State
Conformational states are shown to exist as a structurally heterogeneous ensemble within a single ring.
Monday, July 22, 2013
Crystal Structure of Signal Regulatory Protein Gamma in Complex with an Antibody Fab Fragment
This paper presents the X-ray crystal structure of the complete extracellular portion of SIRP? co-crystallized with the Fab fragment of the OX117 monoclonal antibody.
Tuesday, July 09, 2013
The X-ray Crystal Structure of Mannose-Binding Lectin-Associated Serine Proteinase-3
These data reveal substantial perturbation of the active site, consistent with a correlation between the lack of MASP-3 function and the 3MC syndrome.
Thursday, June 27, 2013
Crystal Structure of the Capsular Polysaccharide Synthesizing Protein CapE of Staphylococcus Aureus
This Study presents the first crystal structure of CapE, revealing that this enzyme forms a stable and functional homo-hexamer.
Thursday, May 02, 2013
Scientific News
3-D Atomic Structure of Cholesterol Transporter
Researchers at UTSW have determined the 3-D atomic structure of a human sterol transporter that helps maintain cholesterol balance.
Neutron Analysis of HIV-1 Protease
Neutrons probe structure of enzyme critical to development of next-generation HIV drugs.
Do Germs Cause Type 1 Diabetes?
Germs could play a role in the development of type 1 diabetes by triggering the body’s immune system to destroy the cells that produce insulin, new research suggests.
Crucial Reaction for Vision Revealed
Scientists have tracked the reaction of a protein responding to light, paving the way for a new understanding of life's essential reactions.
Secrets of a Deadly Virus Family Revealed
Scripps Research scientists uncover the glycoprotein structure of LCMV. The findings could guide development of treatments for Lassa fever.
Serotonin Transporter Structure Revealed
Researchers determined the 3-D structure of the serotonin transporter and visualized how two common antidepressants interact with the protein.
Zika Virus Structure Revealed
Team at Purdue becomes the first to determine the structure of the Zika virus, which reveals insights critical to the development of effective antiviral treatments and vaccines.
Half a Million-Dollar Tick
How proteins present in tick saliva prevent the immune system from running amok.
Promising Model for Hantavirus Drug Design
X-ray crystallography provides drug template against disease transmitted by small rodents.
A Crystal Clear View of Biomolecules
Fundamental discovery triggers paradigm shift in crystallography.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!