Corporate Banner
Satellite Banner
Crystallography
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Whitepaper from Wyatt on Branching in Synthetic and Natural Polymers

Published: Tuesday, February 11, 2014
Last Updated: Monday, February 10, 2014
Bookmark and Share
New whitepaper highlights the importance of multi-angle light scattering instrumentation.

Wyatt Technology Corporation has published a new whitepaper on characterizing molecular structure in synthetic and natural polymers by multi-angle light scattering.

Authored by Stepan Podzimek, Scientific Consultant for Wyatt Technology, the paper details the basic principles of the detection and characterization of branching by means of a multi-angle light scattering (MALS) detector and the various methods used.

To obtain a copy of the study please click here or visit the application note library available at www.wyatt.com.

The whitepaper outlines research into the abnormal elution behavior of branched macromolecules in size exclusion chromatography (SEC) and explains the comparative data acquired by SEC-MALS and asymmetric flow field flow fractionation (A4F).

The comparison of SEC-MALS and A4F-MALS proves superior, artifact-free A4F separation of large and highly branched macromolecules compared to their abnormal separation by SEC.

Highlighting the significant impact branching has on the properties of the structural parameter of synthetic and natural polymers; the whitepaper acknowledges the importance of quantitative branching topology information, for the development of novel polymer-based materials and for understanding polymerization processes.

Using Wyatt Technology instruments: a MALS photometer DAWN® HELEOS®, an online viscometer ViscoStar™, a refractive index detector Optilab® T-rEX™ and an A4F system Eclipse™ AF4; Dr. Podzimek successfully analyzed branching in poly(lactic acid).

Wyatt instrumentation provided a solution to previous challenges in branching studies, with the characterization of branching by means of size exclusion chromatography (SEC) or asymmetric flow field flow fractionation (A4F), previously not possible without a multi-angle light scattering detector.

Stepan Podzimek comments; “In order to identify and characterize branching, one needs information about the molar mass and the molecular size. That is exactly the power of using a multi-angle light scattering detector: it provides both pieces of information simultaneously and independently. The MALS detector is usually connected to an analytical separation technique - most commonly SEC - to determine branching parameters as a function of molar mass. Typically the parameters of interest are the branching ratio and the number of branch units per molecule. Wyatt MALS photometers allow direct determination of branching ratio g and number of branch units per molecule.”

The MALS photometer DAWN® HELEOS®, used in the study, is an 18-angle light scattering detector for the measurement of absolute molecular weight, size, and conformation of macromolecules in solution.

Used specifically in this instance, to detect branching in hyaluronic acid, an important biopolymer with numerous media and pharmaceutical applications which was until recently believed to have a linear structure; the DAWN® can be used for continuous flow detection following chromatographic separation, or off-line as a stand-alone unit in batch or microbatch mode.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Using Appropriate Buffer Conditions Improves Sample Preparation for Structural Analysis
New Customer Application Note Available from Wyatt Technology.
Monday, July 29, 2013
Wyatt Technology Ranked Among The Scientist Magazine’s Best Places to Work in Industry
Wyatt was named #5 on the worldwide list of companies this year.
Tuesday, July 02, 2013
Wyatt Technology Celebrates 30th Years
Company will toast 300 years of macromolecular characterization expertise at the 2012 International Light Scattering Colloquium.
Thursday, July 12, 2012
Wyatt Technology Ranks as #4 in The Scientist’s 10th Annual Best Places to Work Survey
Company ranked fourth place due to their ability to nurture scientific excellence and provide high staff satisfaction.
Monday, June 04, 2012
MALS Detectors Used in Breakthrough Virus Research
Wyatt Technology Corporation have announced that its instruments were used in a recent landmark study that transforms the previous scientific understanding of immunity to viral diseases like the common cold, 'winter vomiting' and gastroenteritis.
Wednesday, January 05, 2011
Wyatt Technology to Host 21st Annual International Light Scattering Colloquium
The annual event will showcase Wyatt’s new Möbiu? mobility instrument.
Thursday, August 19, 2010
Wyatt Technology Uses Composition Gradient Multi-Angle Light Scattering for Characterizing Protein Inhibition in Solution Without Labeling or Immobilization
Wyatt Technology Corporation, announces the availability of a new application note demonstrating the advanced capability of Composition Gradient Multi-angle Light Scattering (CG-MALS) technology.
Wednesday, September 23, 2009
Wyatt Technology Showcases Advanced Solutions at 234th ACS 2007
Wyatt will be showcasing its range of solutions including DynaPro Plate Reader with temperature control on 234th ACS meeting and exposition.
Friday, May 11, 2007
Wyatt Demonstrates Strength in Macromolecular Characterization Solutions
Wyatt will be exhibiting its comprehensive range of products on booth #1944, PittCon 2006.
Monday, December 26, 2005
Scientific News
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
New Mathematics Advances the Frontier of Macromolecular Imaging
Berkeley Lab’s M-TIP solves the reconstruction problem for fluctuation X-ray scattering.
Diamond Helps Develop New Way of Studying the Tiniest Microcrystals
Researchers have developed a new type of sample holder for ‘serial protein crystallography’.
Crystal Clear Images Uncover Secrets of Hormone Receptors
NIH researchers gain better understanding of how neuropeptide hormones trigger chemical reactions in cells.
TOPLESS Plants Provide Clues to Human Molecular Interactions
Scientists at Van Andel Research Institute have revealed an important molecular mechanism in plants that has significant similarities to certain signaling mechanisms in humans, which are closely linked to early embryonic development and to diseases such as cancer.
Advancing Cancer Drug Design with Image of Key Protein
Scientists have pioneered the use of a high-powered imaging technique to picture in exquisite detail one of the central proteins of life – a cellular recycling unit with a role in many diseases.
Mould Unlocks New Route to Biofuels
Scientists at The University of Manchester have made an important discovery that forms the basis for the development of new applications in biofuels and the sustainable manufacturing of chemicals.
'Invisible' Protein Structure Explains the Power of Enzymes
A research group at Umeå University in Sweden has managed to capture and describe a protein structure that, until now, has been impossible to study.
Unraveling the Elusive Structure of HIV Protein
Snapshots of HIV virus’ proteins may help design new ways to fight the disease.
Blueprinting Cell Membrane Proteins
Recent breakthrough will make the blueprinting process faster, easier and cheaper, and should have major implications in the field of drug discovery and development.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!