Corporate Banner
Satellite Banner
Crystallography
Scientific Community
 
Become a Member | Sign in
Home>Videos>This Video
  Videos

Return

Old Structures - New Targets for Antivirulence Salicylidene Acylhydrazide Compounds
Select Biosciences Ltd

Salicylidene acylhydrazide compounds have been demonstrated to perturb the function of the type three secretion of many important human pathogens including Escherichia coli O157 and Yersinia pseudotuberculosis. The actual protein targets of these compounds had, to this date, been unknown. In this work we present the identification and verification of three such protein targets. Each protein was identified using a combination of affinity chromatography and Far-western analyses followed by phenotypic testing of defined bacterial mutants using in vitro and in vivo assays. , , The structure of the target protein with the greatest effect on virulence in Yersinia pseudotuberculosis was solved in 3 forms by X-ray crystallography. In combination with studies of inactive mutants, and conformational changes in the protein caused by redox active state, we present localised regions likely to bind to the drug. Work is under way to solve the structure of protein and compound that will permit refinement of the salicylidene acylhydrazides with the long-term goal of developing clinically-useable drugs.

Request more information
Company product page

Scientific News
Crystal Clear Images Uncover Secrets of Hormone Receptors
NIH researchers gain better understanding of how neuropeptide hormones trigger chemical reactions in cells.
TOPLESS Plants Provide Clues to Human Molecular Interactions
Scientists at Van Andel Research Institute have revealed an important molecular mechanism in plants that has significant similarities to certain signaling mechanisms in humans, which are closely linked to early embryonic development and to diseases such as cancer.
Advancing Cancer Drug Design with Image of Key Protein
Scientists have pioneered the use of a high-powered imaging technique to picture in exquisite detail one of the central proteins of life – a cellular recycling unit with a role in many diseases.
Mould Unlocks New Route to Biofuels
Scientists at The University of Manchester have made an important discovery that forms the basis for the development of new applications in biofuels and the sustainable manufacturing of chemicals.
'Invisible' Protein Structure Explains the Power of Enzymes
A research group at Umeå University in Sweden has managed to capture and describe a protein structure that, until now, has been impossible to study.
Unraveling the Elusive Structure of HIV Protein
Snapshots of HIV virus’ proteins may help design new ways to fight the disease.
Blueprinting Cell Membrane Proteins
Recent breakthrough will make the blueprinting process faster, easier and cheaper, and should have major implications in the field of drug discovery and development.
Bacteria Use Chemical Harpoons to Hold on Their Hosts
Researchers reveal how a common disease causing bacteria latches on to the body during an infection.
Solving Streptide from Structure to Biosynthesis
Researchers reveal new information about how bacteria communicate via the protein, streptide.
Near-Atomic Resolution of Protein Structure Holds Promise for Drug Discovery
A new study shows that it is possible to use an imaging technique called cryo-electron microscopy to view the architecture of a metabolic enzyme bound to a drug that blocks its activity.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!