Corporate Banner
Satellite Banner
Molecular & Clinical Diagnostics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Cell-Detection System Promising for Medical Research, Diagnostics

Published: Thursday, October 03, 2013
Last Updated: Thursday, October 03, 2013
Bookmark and Share
Researchers are developing a system that uses tiny magnetic beads to quickly detect rare types of cancer cells circulating in a patient's blood.

While other researchers have used magnetic beads for similar applications, the new “high-throughput" system has the ability to quickly process and analyze large volumes of blood or other fluids, said Cagri Savran (pronounced Chary Savran), an associate professor of mechanical engineering at Purdue University.

He is working with oncologists at the Indiana University School of Medicine to further develop the technology, which recently was highlighted in the journal Lab on a Chip.

The approach combines two techniques: immunomagnetic separation and microfluidics. In immunomagnetic separation, magnetic beads about a micron in diameter are "functionalized," or coated with antibodies that recognize and attach to antigens on the surface of target cells.

The researchers functionalized the beads to recognize breast cancer and lung cancer cells in laboratory cultures.

"We were able to detect cancer cells with up to a 90 percent yield," said Savran, working with Purdue postdoctoral fellow Chun-Li Chang and medical researchers Shadia Jalal and Daniela E. Matei from the IU School of Medicine's Department of Medicine. "We expect this system to be useful in a wide variety of settings, including detection of rare cells for clinical applications."

Previous systems using immunomagnetic separation to isolate cells required that the cells then be transferred to another system to be identified, counted and studied.

"What's new here is that we've built a system that can perform all of these steps on one chip," said Savran, also an associate professor of biomedical engineering. "It both separates cells and also places them on a chip surface so you can count them and study them with a microscope."

Another innovation is the fast processing, he said. Other "microfluidic" chips are unable to quickly process large volumes of fluid because they rely on extremely narrow channels, which restrict fluid flow.

"The circulating cancer cells are difficult to detect because very few of them are contained in blood," Savran said. "That means you have to use as many magnetic beads as practically possible to quickly screen and process a relatively large sample, or you won't find these cells."

The new design passes the fluid through a chamber that allows for faster flow; a standard 7.5-milliliter fluid sample can run through the system in a matter of minutes.

The Purdue portion of the research is based at the Birck Nanotechnology Center in Purdue's Discovery Park.

The beads are directed by a magnetic field to a silicon mesh containing holes 8 microns in diameter. Because the target cells are so sparse, many of the beads fail to attract any and pass through the silicon mesh. The beads that have attached to cells are too large to pass through the holes in the mesh.

If needed, the cells can quickly be flushed from the system for further analysis simply by turning off the magnetic field.

"Not only can the cells be readily retrieved for further usage, the chip can be re-used for subsequent experiments," Savran said.

The technology also could be used to cull other types of cells.

"This is not only for cancer applications," he said.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Microsecond Raman Imaging Might Probe Cells, Organs for Disease
An advanced medical diagnostic tool for the early detection of cancer and other diseases.
Thursday, April 02, 2015
Keck Foundation to Fund Purdue Research into Spectroscopic Imaging
Ji-Xin Cheng leads a Purdue team awarded a $1 million W.M. Keck Foundation grant to develop a new type of imaging technology for cell and tissue analysis that could bring advanced medical diagnostics.
Tuesday, February 10, 2015
Purdue to Accelerate Drug Discovery, Development
Purdue Center for Drug Discovery adds to Purdue's strengths and the capacity to translate basic research into life-changing treatments.
Monday, September 16, 2013
Indiana Biosciences Research Institute Unveiled
Purdue University part of first industry-led collaborative life sciences research institute in the United States.
Monday, June 10, 2013
New Imaging Technology Could Reveal Cellular Secrets
Researchers have married two biological imaging technologies, creating a new way to learn how good cells go bad.
Friday, April 26, 2013
MolecularHUB Gives Scientific Information on Fast-moving Diseases
A scientific gateway website that will provide molecular and genetic information on infectious and emerging diseases has been released by Purdue University.
Thursday, October 25, 2012
Sensor Detects Glucose in Saliva and Tears for Diabetes Testing
Researchers have created a new type of biosensor that can detect minute concentrations of glucose in saliva, tears and urine and might be manufactured at low cost because it does not require many processing steps to produce.
Thursday, September 13, 2012
Scientific News
Liquid Biopsies: Utilization of Circulating Biomarkers for Minimally Invasive Diagnostics Development
Market Trends in Biofluid-based Liquid Biopsies: Deploying Circulating Biomarkers in the Clinic. Enal Razvi, Ph.D., Managing Director, Select Biosciences, Inc.
Ultrafast DNA Diagnostics
New technology developed by UC Berkeley bioengineers promises to make a workhorse lab tool cheaper, more portable and many times faster by accelerating the heating and cooling of genetic samples with the switch of a light.
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
Paving the way to Better Ovarian Cancer Diagnosis
Aïcha BenTaieb will present her invention for automated identification of ovarian cancer’s many subtypes at an international conference this fall.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
Diagnostic Test Developed for Enterovirus D68
researchers at Washington University School of Medicine in St. Louis have developed a diagnostic test to quickly detect enterovirus D68 (EV-D68), a respiratory virus that caused unusually severe illness in children last year.
Tracking Breast Cancer Before it Grows
A team of scientists led by University of Saskatchewan researcher Saroj Kumar is using cutting-edge Canadian Light Source techniques to screen and treat breast cancer at its earliest changes.
The Light of Fireflies for Medical Diagnostics
EPFL scientists have exploited the light of fireflies in a new method that detects biological molecules without the need for complex devices and high costs.
Could a simple saliva test detect Alzheimer's?
Researchers have presented findings suggesting that a simple, non-invasive diagnostic for Alzheimer's could be within reach.
Cheap Diagnostics with a Portable "Paper Machine"
Scientists have developed a cheap, portable system for point of care diagnostics for a range of infectious diseases, genetic conditions and cancer.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!