|Building a digital pathology ecosystem for education and research|
Yves Sucaet, Silke Smeets, Stijn Piessens, Sabrina D'Haese, Chris Groven, Wim Waelput, Peter In't Veld
We wanted to build a core digital pathology infrastructure to support different use cases. Various images platforms needed to be accessible through a single access point, and support different user profiles. We wanted a scalable solution that would allow interaction between equipment from different research groups.
We built a centralized infrastructure that integrates a variety of imaging platforms, and now have an interconnected network of heterogeneous and scalable information silos.
|Development of a nucleic acid-based amplification test as point-of-care rapid test detecting sexually-transmitted diseases for home-care|
Vicky Tröger, Anja Scherber, Dirk Kuhlmeier, Indrek Tulp, Marko Lehes, Steffen Howitz, Toni Bohatzsch, Tamas Pardy
The main problem of early diagnosis of STI is the lack of suitable systems. Therefore a quick home test is needed. In this poster, a microfluidic cartridge combining an isothermal amplification with detection on a lateral flow strip is presented.
|A Microwave/Microfluidics Integrated Label-Free Detection and Content Sensing System for Point-of-Care Applications|
Gurkan Yesiloz, Muhammed S. Boybay, Carolyn L. Ren
This poster presents a droplet-based microfluidic device integrated with a microwave wave sensor, which is also a heater, for simultaneous sensing and heating of individual nanoliter-sized droplets.
|LOHA Comprehensive Assay for Single Nucleotide Polymorphism, Copy Number Variants and Loss of Heterozygosity Using SureSelect Target Enrichment|
Kyeong Soo Jeong, Arjun Vadapalli, Ashutosh Ashutosh, Paula Costa, Brian Peter, Stephanie Fulmer-Smentek, Magnus Isaksson, Jayati Ghosh, Douglas Roberts, Holly Hogrefe
Here we describe a comprehensive assay that enables researchers to identify SNP, INDEL, CNV, and LOH using SureSelect target enrichment. This design can be employed as a standalone entity or in concert with other bait designs for SNP and INDEL detection. We also describe methods for data analysis and visualization.
|Alterations in serum thrombotic biomarker in patients with acute myocardial infarction |
Abdullah S. Alhomida
A combination of these biomarkers could serve as a useful biomarker tool to assess the prognosis of AMI. (This study was supported by National Plan for Science and Technology Program by King Saud University Project Number 08-BIO571-02).
|Improved Small RNA Library Preparation Workflow for Next-Generation Sequencing|
Sabrina Shore, Jordana Henderson, Anton McCaffrey, Gerald Zon, Richard Hogrefe
We describe an optimized small RNA NGS library prep workflow using chemically modified adapters which suppresses adapter dimers, allows for RNA inputs down to 1 ng and eliminates the need for a gel purification step, thus allowing full automation not previously possible.
|Non-invasive Liquid Biopsy|
Frauke Henjes1, Lise Aagaard1, Darren Ellis1, Hannah Lindstroem1, Morten Oksvold2,3, Axl Neurauter1, Ketil W. Pedersen1.
Paramagnetic Dynabeads® magnetic beads provide a versatile, automation friendly tool for fast sensitive and high throughput isolation that can be tailored to isolate specific circulating biomarkers such as cells, exosomes and nucleic acids.
|Flexible automated platform for blood group genotyping on DNA microarrays|
S. Paris1, D. Rigal1, V. Barlet1, M. Verdier1, N. Coudurier2, P. Bailly3, J.-C. Brès1,4
The purpose of this project was to set up and validate a flexible robotic platform using 96-well DNA microarray for multiplex blood group genotyping.
|ENZYME COATED MAGNETIC NANOPARTICLES FOR USE IN CONTINUOUS FLOW MICROFLUIDIC DEVICES|
Ferenc Ender, Diana Weiser, Andras Vitez, Laszlo Poppe
A novel Lab-on-a-Chip platform is presented incorporates micro-sized magnetic reaction cells capable of anchoring protein-coated MNPs addressable and selectively. Such platform allows the design of highly flexible and "programmable" execution of multienzyme processes for biocatalytic or diagnostic purposes.