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Monday, February 02, 2015
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Diagnostic Significance of microRNA Tumor Biomarkers in Fine Needle Aspirates (FNA) of Pancreatic Cancer
Martina Doleshal 1, Anna E. Szafranska 1, Hayward S. Edmunds 2, Gregory J. Tsongalis 2, Murray Korc 2 and Emmanuel Labourier 1

Collection of pancreatic fine needle aspirates (FNAs) in RNARetain™ allows stabilization and protection of RNA in tissues containing high levels of nucleases; Differential miRNA expression in pancreatic FNAs accurately classifies benign and malignant tissue; Combinations of miRNA and mRNA expression signatures improve the separation between normal tissue and chronic pancreatitis; microRNAs are suitable analytes for molecular characterization of fine needle aspirates from pancreatic tissues.

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A high-throughput protein array-based approach
Rachel Van Dyk, Claudia Kirisits, Paul Potter, Fook Tim Chew, Reinhard Hiller

Here we present a novel discovery-oriented high-throughput approach to the screening of allergen reactivates in crude biological extracts is presented using seafood allergens as an example. The CPGR workflow permits the effective screening of hundreds of putative allergens in parallel using minute amounts of patient serum and constitutes a cost-efficient allergen-specific antibody screening method for a routine diagnostic setting.

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Accuwik® Ultra Membrane for the Direct Collection, Storage, and Efficient Rapid Release of Biological Samples for Clinical Diagnostics
Galina Fomovska, Stephane Perreault, Richmond Lo, and Martin A. Smith

Poster data assesses use of Pall’s Accuwik Ultra membrane as a reliable tool for direct collection of clinical samples including saliva and plasma, enables stability during sample storage, and allows for rapid and efficient release of clinically relevant protein biomarkers suitable for diagnostic analyses.

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Expression of stress response protein GRP78 is associated with the development of castration-resistant prostate cancer
Llana Pootrakul, Anirban P. Mitra, Ram H. Datar, Shan-Rong Shi, Jie Cai, Debra Hawes, Susan G. Groshen, Amy S. Lee, Richard J. Cote

This study investigated the role of the GRP78 in prostate cancer progression and the development of castration-resistant prostate cancer, where cancer cells continue to survive despite the stress of an androgen-starved environment.

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Application of genetic programming in analysis of quantitative gene expression profiles for identification of nodal status in bladder cancer
Anirban P. Mitra, Arpit A. Almal, Ben George, David W. Fry, Peter F. Lenehan, Vincenzo Pagliarulo, Richard J. Cote, Ram H. Datar, William P. Worzel

Nodal involvement in bladder cancer is an independent indicator of prognosis. This study employed an iterative machine learning process called genetic programming on quantitative expression values of 70 genes to classify primary urothelial carcinoma samples into those associated with or without nodal metastasis. The generated rules showed a strong predilection for ICAM1, MAP2K6 and KDR resulting in gene expression motifs that cumulatively suggested a pattern ICAM1>MAP2K6>KDR for node positive ca

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Interactive Transcription Maps Over Microarray Data
Petr Krontorad, Irena Koutna and Michal Strehovsky

For final description of microarray experiment results it is necessary to use some statistically robust method. Transcription maps (TM) are used to map results of gene expression analysis into individual chromosomes. We have implemented graphical tool for interactive display of TMs. This tool allows displaying of arbitrary information for each gene.

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Whole Body Endogenous Nitric Oxide Production in Patients with Decompensated Liver Disease
Demoncheaux E., Elphick D.A., Dürner M.B., Higgins G.E., Crowther D., Williams E.J., Higenbottam T.W and Gleeson D

Increased nitric oxide (NO) production has been implicated in the pathogenesis of the hyperdynamic circulation found in patients with advanced liver disease. There were no differences between patients with liver disease and controls with regard whole body NO production. Our results, in a well characterised set of patients, argue against greater basal NOS-dependent whole body NO production in patients with decompensated liver disease.

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Caveolin-1 Expression as a Possible Biomarker in Pancreatic Cancer Diagnosis
C. Tanase, E. Raducan, L. Albulescu, E. Codorean, M.I. Nicolescu, D.I. Popescu, M.L. Cruceru and A.C. Popa

Caveolin1 (Cav-1) function either as a tumor supressor or as a promoter of metastasis. Overexpresion of cav-1 was correlated with: tumoral grading, proliferration markers (Ki67, p53), serum tumor markers (CEA, CA19.9) and angiogenic markers (VEGF, bFGF).

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A Novel Miniaturized Cell Lysis Device Using Spherically Focused Ultrasound
Gang Li, Hong Xiao, Min Guo and Jing Cheng

A prototype of miniaturized cell lysis device has been developed using a spherically concave transducer, which is capable of lysing bacteria in absence of added chemical denaturants or enzymes and lysing yeast efficiently without any mechanical or enzymatic pretreatment.

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Showing Results 41 - 50 of 60
Scientific News
NIH Launches Tool to Advance Down Syndrome Research
Web portal will help approved professionals to plan clinical studies.
New Blood Test For Aggressive Bone Cancer
Researchers at the University of East Anglia are developing a simple blood test to diagnose bone cancer thanks to funding from the Big C.
Novel Approach to Visualize & Measure Protein Complexes in Tumors
Moffitt Cancer Center researchers have developed a PLA to analyze biological processes occurring within tumors.
Prognostic Test for E2F4 in Breast Cancer Developed
A gene signature in E2F4 has been identified which is predictive of estrogen receptor positive breast cancer.
Blood Test For Prostate Cancer
Vanderbilt team demonstrates that prostate cancer can accurately be detected by liquid biopsy.
Reducing Risk of Miscarriage in Prenatal Testing for Genetic Diseases
Early diagnosis and treatment are crucial to improving patient prognosis for 22q11 deletion syndrome and Wilson’s disease, two potentially life-threatening genetic conditions.
Predicting Sepsis
Altered white-blood-cell motion in burn patients may warn of infection.
Measuring Brain Volume can Help People with Multiple Sclerosis
icometrix has developed software for measuring the brain and brain lesion volume.
Metal Test Could Help Diagnose Breast Cancer Early
It may be possible to develop a simple blood test that, by detecting changes in the zinc in our bodies, could help to diagnose breast cancer early.
Gene Associated with an Aggressive Breast Cancer Identified
Over-expressed gene in triple negative breast cancer offers new diagnostics for risk assessment.
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