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General V3 Western Blotting Protocol
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Bio-Rad Laboratories

Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls. 

Bio-Rad has developed the V3 Western Workflow™ utilizing stain-free technology to address the major concerns associated with the traditional western blotting procedure. This application note describes the V3 Western Workflow affording faster, more transparent, quantitative, and more reliable western blotting.

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