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SPE Method to Recover Low Concentrations of Microcystins in Water

Published: Thursday, November 18, 2010
Last Updated: Thursday, November 18, 2010
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A newly developed method that can fully recover low concentrations (< 1 µg/L) of three microcystins (-RR, -YR, and -LR) when added to water samples has been announced by Dionex.

This new method is detailed in Application Note 261: Sensitive Determination of Microcystins in Drinking and Environmental Waters and demonstrates how recovery at lower concentrations than the maximum recommended by the World Health Organization (WHO) can be achieved. This method is fully automated and easily configured on an UltiMate® 3000 ×2 Dual HPLC system, using Acclaim® PA2 columns for solid-phase extraction (SPE) and analysis.

Microcystin contamination of drinking water at low nanomolar concentrations is considered a risk factor for cancer; therefore, WHO has proposed a provisional guideline concentration of 1.0 μg/L for microcystin-LR in drinking water. This new target-cut on-line SPE method with dual function (analyte capture and partial separation) operates under automatic control of Chromeleon® Chromatography
Data System (CDS) software and offers full automation, absence of operator influence, and strict process control.


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