Corporate Banner
Satellite Banner
Environmental Analysis
Scientific Community
Become a Member | Sign in
Home>News>This Article

Characterization of Lead-Phytochelatin Complexes by Nano-Electrospray Ionization Mass Spectrometry

Published: Friday, March 02, 2012
Last Updated: Friday, March 02, 2012
Bookmark and Share
This study describes the development of a nano-electrospray ionization mass spectrometry method for the identification and characterization of Lead(II) and Zinc(II) phytochelatin complexes.

The role of phytochelatins (PC(n), metal-binding oligopeptides with the general structure (γGlu-Cys)(n)-Gly (n = 2-11) in metal detoxification is assumed to be based on immobilization of metals, which prevents binding of metals to important biomolecules. Although induction of phytochelatin synthesis has often been observed in algae upon exposure to metals, direct evidence for binding of the inducing metal to phytochelatins is scarce. In this study, a nano-electrospray ionization mass spectrometry (nano-ESI-MS) method is developed for identification and characterization of Pb(II)-PC(n) and Zn(II)-PC(n) complexes. Complexes of Pb(II) with standard PC(n) (n = 2-4; 0.25 mM Pb(II) and 0.5 mM PC(n)) were examined by nano-ESI-MS with respect to their stoichiometry. Pb-PC(n) mass spectra indicated the presence of the [M + H](+) peak of PC(n) and complexes with various stoichiometries. Analysis of Pb-PC(2) allowed the identification of four different complexes observed at m/z 746.10, 952.06, 1285.24, and 1491.20, corresponding to [Pb-PC(2)](+), [Pb(2)-PC(2)](+), [Pb-(PC(2))(2)](+), and [Pb(2)-(PC(2))(2)](+). Their m/z indicated coordination of Pb(II) by PC(2) through the thiol groups of PC cysteine and possibly carboxylic groups. For each of the standard PC(3) and PC(4), two different complexes were observed, corresponding to Pb-PC(3), Pb(2)-PC(3), Pb-PC(4), and Pb(2)-PC(4). The measured isotopic patterns were for all complexes identical to the theoretical isotopic patterns. Addition of Zn(II) (0.125-5 mM) to previously formed Pb-PC(2) complexes showed the appearance of the [Zn-PC(2)](+) complexes at m/z 602.05 and the decrease of the [Pb-PC(2)](+) peak. These findings corroborate the postulated Pb-PC complexes from a previous study using size exclusion chromatography of PC extracted from algae, as well as the concurrent formation of Pb-, Zn-, and Cu-PC complexes in algae.

The article is published online in Frontiers in Microbiology and is free to access.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,700+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Simultaneous Determination of Six Flame Retardants in Water
The work aimed to develop an analytical procedure for the extraction and simultaneous determination of selected flame retardants.
Thursday, July 24, 2014
Comparison of Pesticide Residues in Surface and Ground Water
Measurement of pesticide residues can be used as baseline levels to monitor the future changes and to predict their future impact on the population of the area.
Thursday, May 15, 2014
Simultaneous Determination of Hormonal Residues in Treated Waters using UHPLC-MS/MS
The identification of hormone residues in environment is of special interest because as this is a requirement to take measures in order to regulate and minimize their environmental impact.
Tuesday, April 09, 2013
A Rapid uHPLC Method for the Simultaneous Determination of Drugs from Different Therapeutic Groups in Surface Water and Wastewater
The method can be successfully applied to the determination of selected drugs in water samples and can be used as a monitoring tool for water pollution in rivers and WWTPs effluents.
Tuesday, February 12, 2013
A Summary Catalogue of Microbial Drinking Water Tests for Low and Medium Resource Settings
Researchers from the University of Bristol present the first comprehensive catalogue of the characteristics of available and emerging low-cost tests for faecal indicator bacteria.
Wednesday, July 25, 2012
Global Access to Safe Water
This study highlights the substantive differences between population estimates using water from an improved source and estimates of populations using water free of faecal contamination and water with low risk of faecal contamination.
Friday, July 06, 2012
On-line Concentration and Fluorescence Determination HPLC for PAHs in Seawater Samples and its Application to Japan Sea
This method, developed by researchers at Kanazawa University, allowed measurements in the low ng/g range, with high recoveries and a good intraday and interday repeatability.
Wednesday, May 16, 2012
Permitted Water Pollution Discharges and Population Cancer and Non-Cancer Mortality
The study conducts statistical and spatial analyses to investigate amounts and types of permitted surface water pollution discharges in relation to population mortality rates for cancer and non-cancer causes in US rural-urban areas.
Tuesday, May 01, 2012
Development of a Screening Method for the Determination of PCBs in Water
This novel method utilises gas chromatography coupled to triple quadrupole mass spectrometry for the simultaneous analysis of 11 polychlorinated biphenyls.
Thursday, April 05, 2012
The Water Footprint of Humanity
By showing that several countries heavily rely on foreign water resources which significantly impacts water consumption and pollution elsewhere, researchers from the University of Twente illustrate the global dimension of water consumption and pollution.
Thursday, March 01, 2012
Analysis of Bromate in Drinking Water using Liquid Chromatography-Tandem Mass Spectrometry without Sample Pretreatment
Researchers from the National Institute of Public Health have developed an analytical method for determining bromate in drinking water using liquid chromatography-tandem mass spectrometry.
Thursday, December 01, 2011
Detection of Stanozolol in Environmental Waters Using Liquid Chromatography Tandem Mass Spectrometry
Researchers from Kingston University have developed an efficient, rapid, reproducible, sensitive and robust method for the detection of stanozolol in aqueous matrices.
Wednesday, October 19, 2011
Retrospective Exposure Estimation and Predicted versus Observed Serum Perfluorooctanoic Acid Concentrations for Participants in the C8 Health Project
Serum perfluorooctanoic acid concentrations predicted by linked exposure and ADME models were shown to correlate well with observed 2005-2006 human serum concentrations for C8 Health Project participants.
Friday, September 16, 2011
Bacterial Colonization of Pellet Softening Reactors used during Drinking Water Treatment
This article describes the opportunistic bacterial colonization of the calcite pellets in a full-scale pellet softening reactor and the functional contribution of these colonizing bacteria to the overall drinking water treatment process.
Friday, August 05, 2011
Solid-Phase Extraction of Antipyrine Dye for Spectrophotometric Determination of Phenolic Compounds in Water
In this article researchers from Yokohama National University have a proposed a novel method for the determination of phenolic compounds in water.
Monday, June 27, 2011
Scientific News
First Results Describing Sick Sea Star Immune Response
Though millions of sea stars along the West Coast have perished in the past several years from an apparent wasting disease, scientists still don’t know why.
Microbe Sleuth
Tanja Bosak examines how life and the Earth evolved in tandem during their early history together.
The Age of Humans Controlling Microbes
Engineered bacteria could soon be used to detect environmental toxins, treat diseases, and sustainably produce chemicals and fuels.
Messing With The Monsoon
Manmade aerosols can alter rainfall in the world’s most populous region.
Plastic for Dinner
Roughly a quarter of the fish sampled from fish markets in California and Indonesia contained man-made debris according to a study from the University of California, Davis, and Hasanuddin University in Indonesia.
Seeking “Gold Standard” Wastewater Treatments
Metagenomic analyses lend insights into how microbes break down wastewater contaminants.
Preventing Drinking Water Contamination by Pharmaceuticals
In recent years, researchers have realized that many products, including pharmaceuticals, have ended up where they’re not supposed to be — in our drinking water.
Low-level Arsenic Exposure Before Birth Associated with Early Puberty in Female Mice
Study examine whether low-dose arsenic exposure could have similar health outcomes in humans.
Intensity of Desert Storms May Affect Ocean Phytoplankton
MIT study finds phytoplankton are extremely sensitive to changing levels of desert dust.
Determination of Phosphate in Soil Extracts in the Field: A Green Chemistry Enzymatic Method
New method for phosphate determination which can be carried out in the field to obtain results on the spot.
Scroll Up
Scroll Down

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,700+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos