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Sample preparation with the AC Extraction Plate: extraction of urinary THC metabolites for LC-MSMS quantification
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Tecan Group Ltd.

The metabolite THC-COOH was successfully determined from urine matrix by a combination of AC Extraction Plate for sample preparation followed by LC-MSMS quantification. The range of THC-COOH calibrator concentrations covered the forensically relevant range. 

The AC Extraction Plate featuring TICE technology is a fast and easy-to-automate sample preparation method requiring minimal sample pre-treatment. No time consuming or rather costly to automate procedures – such as protein precipitation with centrifugation, filtration or evaporation – are needed thus accelerating and simplifying the entire workflow. 

This application note describes a novel sample preparation method for the extraction of THC-COOH from urine for subsequent LC-MSMS analysis, based on Tecan Immobilized Coating Extraction (TICE™) technology. 

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