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Webcasts
Webcasts - Romantic Surfaces and Microbial CaptureRomantic Surfaces and Microbial Capture
Han Zuilhof, Wageningen University, speaking at Biodetection & Biosensors 2015
No image available for this webcastHistory and Development of High Resolution Mass Spectrometry for Infectious Disease Diagnostics
Haroun Shah, Public Health England, speaking at Clinical Applications of Mass Spectrometry.

Market Reports
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Scientific News
Low-Cost, Portable NQR Spectroscopy
A researcher at Case Western Reserve University is developing a low-cost, portable prototype designed to detect tainted medicines and food supplements that otherwise can make their way to consumers. The technology can authenticate good medicines and supplements.
Chemical Used to Replace BPA is Potentially Toxic
This study is the first to examine the effects of BPA and BPS on brain cells and genes that control the growth and function of organs involved in reproduction.
Toxic Pollutants Found in Fish Across the World's Oceans
Scripps researchers' analysis shows highly variable pollutant concentrations in fish meat.
Weight-loss Supplements Containing Raspberry Ketone May Be Harmful
New study by the researchers suggests that raspberry ketone may have several adverse effects.
Quantifying C. botulinum Spores
A study from the Institute of Food Research has provided new evidence on the background levels of spores of Clostridium botulinum in raw food ingredients that is helping the food industry deliver safe chilled foods more sustainably.
Single Molecule Detection of Contaminants, Explosives or Diseases
A technique that combines the ultrasensitivity of surface-enhanced Raman scattering (SERS) with a slippery surface invented by Penn State researchers will make it feasible to detect single molecules of a number of chemical and biological species from gaseous, liquid or solid samples.
Should I Throw Away Food Once a Fly Has Landed on it?
Flies can be a substantial annoyance and may also be a potential health risk.
New Year, Old Beer
A bottle of Alexander Keith’s beer has been keeping Dal Engineering prof Andrew MacIntosh busy at work this week.
Sensor Detects Toxins Leaching from Plastic
Engineers from Massey University have developed a highly sensitive device able to detect synthetic compounds that leach from plastic food packaging into the contained food or beverage.
New BPA Detection Tests
Scientists from the JRC developed fit-for-purpose analytical methods for the determination of bisphenol A and 12 similar substances (analogues) in food matrices to support possible future monitoring studies.
Business News
Agilent Wins Award for Innovation in LC
Dual-needle technology enhances speed, flexibility.
Canadian Government Invests in Species ID Research
Canadian Minister of Health Jane Philpott announced at the University of Guelph today a new federal investment in genomics research to improve plant protection and food safety regulation.
Innate® Second Generation Potato Receives FDA Safety Clearance
The Food and Drug Administration has completed its food and feed safety assessment of the J.R. Simplot Company’s second generation of Innate® potatoes.
It is All About the Product, Right?
Technology Networks met with Waters Corporation at its head office in Milford, MA to better understand the factors they use to drive business success.
US Bans Three Food Packaging Chemicals
New ban includes three specific perfluoroalkyl ethyl containing food-contact substances (FCSs.
Applied Maths Joins the bioMérieux Family
bioMérieux, a world leader in the field of in vitro diagnostics, today announced the acquisition of Applied Maths, a company that develops state-of-the-art software solutions for the biosciences, in particular for databasing, analysis and interpretation of complex biological data.
InstantLabs Launches Two Additional Salmon ID Tests
New species ID tools bolster growing product line from manufacturer of the Hunter® testing system.
Andrew Porter Ltd Completes Relocation of South East Water laboratory
Company has completed the relocation of South East Water to its new purpose built water testing facility and offices in Hampshire, UK.
Shimadzu Wins R&D 100 Award for Nexera Unified Chromatography System
The Nexera UC system is the world’s first ever unified and fully automated system that combines supercritical fluidic extraction (SFE) with supercritical fluid chromatography (SFC).
FDA OK’s AquAdvantage Salmon
After an exhaustive and rigorous scientific review, FDA has arrived at the decision that AquAdvantage salmon is as safe to eat as any non-genetically engineered (GE) Atlantic salmon, and also as nutritious.

Events
The Probiotics Congress: Asia
29 Feb 2016
The Probiotics Congress: Asia ...
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Pittcon 2016
06 Mar 2016
Pittcon 2016 ...
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Paperless Lab Academy
19 Apr 2016
Paperless Lab Academy ...
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Food Allergy Summit 2016
05 Jul 2016
Food Allergy Summit 2016 ...
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ePosters
A Simple Plate Based Assay Using pH Sensor Dye to Screen for Internalizing Antibody
Promega Corporation

Receptor mediated internalization is a key mechanism of action (MOA) for antibody drug conjugates (ADCs). However, current methods of studying antibody internalization have several limitations including: 1) A multistep process not suitable for screening; 2) Low signal-to-background ratios; 3) Not suitable for kinetic measurements. We have developed a method that mitigates problems associated with traditional internalization assays.
An IgG Cleaving Protease from S equi with Improved Activity Against Mouse IgGs
Promega Corporation

Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.
Minimizing Carry-over for High Throughput Analysis
1FHNW Fachhochschule Nordwestschweiz, Hochschule für Life Sciences Gründenstrasse 40 CH-4132 Muttenz; 2CTC Analytics AG Industriestrasse 20 CH-4222 Zwingen; Switzerland

Minimal carry-over is a prerequisite for high throughput analysis. However, minimized carry-over and cycle time are competing and a careful optimization is mandatory. In this study the influence of wash conditions on carry-over of various compounds was investigated. A strategy to minimize carry-over was developed. The influences of different wash tasks were investigated. Finally the contribution of different system components such as injector valve or column was studied.
A Comparison of ITEX Dynamic Headspace–GC/MS to other Enrichment Techniques for Analysis of Flavoring Compounds
1Aspen Research Corporation, Maple Grove, MN; 2CTC Analytics, Zwingen, Switzerland

Enrichment techniques are commonly used for the analysis of flavoring compounds in different matrices with GC/MS. Analysis of flavoring compounds is done by purge & trap, SPME or headspace, depending on requirements for sensitivity. The In-Tube Ex¬traction (ITEX) Dynamic Headspace uses a micro trap filled with an adsorbent material to efficiently extract the compounds. Here we evaluate if the ITEX can be used to effectively analyze for these compounds and reduce the analyst’s time involved.
Automated in-gel digestion on a commercial autosampler directly coupled to nanoLC-MS/MS
University of Bern, Department of Clinical Research, Proteomics & Mass Spectrometry Core Facility, Bern, Switzerland : CTC Analytics AG, Zwingen, Switzerland

SDS-PAGE separates protein samples from LC-MS incompatible contaminations, and is frequently used to fractionate proteins of entire proteomes. One disadvantage is that gel lanes have to be cut into many slices, followed by in-gel digestion of proteins and extraction of peptides. The number of these gel slices goes into the hundreds, rendering this process very repetitive and prone to mistakes and errors during sample handling. Automation reduces such risks and improves reproducibility.
Automated sample preparation workflows for quantitative proteomics applications
1Max Delbrück Center for Molecular Medicine in the Helmholtz Association, MDC, Berlin, Germany; 2CTC Analytics, Zwingen, Switzerland; 3Axel Semrau GmbH, Sprockhövel, Germany

Mass spectrometry based proteomics requires large scale identification of peptides, and depends upon efficient sample preparation. Recently, we presented two automated protein-digestion setups, in-solution and in-gel digestion. We extended these techniques by implementing dimethyl labelling (DML). Furthermore, we established an automated phospho-peptide (PP) enrichment procedure in a 96-well formate, generating phospho-proteomic data in very short time.
Optimization of a Vacuum Ultraviolet Photoionization source for Gas Chromatography used with a High Resolution Time of Flight Mass Spectrometer
LECO Corporation

-Tune solution allows optimization of ion source parameters for
both proton transfer and direct ionization
-Independent ionization processes exist for M+ and MH+
-Optimizing for dopant signal intensity yields inferior results
-Degree of fragmentation remains relatively constant over a
range of source conditions
High Performance Comprehensive Two-Dimensional Gas Chromatography Coupled with a High Resolution Multi-Reflecting TOFMS for Confident Non-Target Analyte Identification
LECO Corporation, Restek Corporation

The use of comprehensive two-dimensional gas chromatography to help increase chromatographic resolution is a major step in tackling the problem of confident peak identification in a complex sample matrix. Combining the separation power of two-dimensional gas chromatography, with resolving power greater than 25,000, and sub ppm mass accuracies of a high resolution multi-reflecting TOFMS is the ideal solution to confident compound identification within a complex sample matrix.
Fragmentation Trees for Automated de novo Interpretation of Impure Electron Ionization Spectra from Gas Chromatographic Complex Mixture Analysis—Chemical Deconvolution
LECO Corporation, Mass Spectrometry Consulting Ltd., Moscow State University

-Chemical deconvolution algorithms accurately reported independent
components of dead coelutions where such components belong to distinct chemical classes.
-Present algorithms fail to distinguish chemically similar analytes such as branched and linear alkanes, thus chemical deconvolution is not a
substitute for GCxGC.
-Chemical deconvolution algorithms accurately rejected more than 80% of spurious signals from manually curated spectra found to be contaminated with unrelated signa
Effective Comparison of Yeast Extracts Using High Resolution GC and GCxGC-HRTOFMS
LECO Corporation

The Pegasus GC-HRT and GC-HRT 4D instruments are effective tools for the comprehensive analysis of yeast extracts.
• Enhanced chromatographic resolution of the HRT 4D system facilitated compound characterization by improving spectral similarity scores
• High quality data resulted in excellent spectral similarity scores when compared to large, well-established databases (NIST, Wiley)
• High resolution, accurate mass data was crucial for determination of fragment, molecular, and adduct
Application Notes
Rapid point of use Grouper Chek™ assay using the Douglas Scientific® AmpliFire® detects seafood fraud
Douglas Scientific

The AmpliFire by Douglas Scientific along with GrouperChek isothermal amplification chemistry from PureMolecular, LLC provide a simple and portable tool to perform genetic analysis at the point of use. This paper demonstrates the ability of the AmpliFire combined with the GrouperChek assay to differentiate the highly prized grouper from tilapia, a common illegal substitute.
Utilization of kinITC in AFFINImeter
Malvern Instruments Limited

This note discusses how and when can kinetic information be retrieved from ITC power curves.
Aseptic Identification of Polysorbates using Handheld Raman
Rigaku Raman Technologies

As polysorbates are reactive to light and air, packaging is usually in thick, dark, amber bottles. This makes the identification process more difficult, as opening the container could compromise the sterility and quality of these materials. Handheld Raman is capable of identifying these materials without any sampling or preparation.
Herbal Materials and Natural Foods Analysis with Handheld Raman
Rigaku Raman Technologies

Dietary supplement manufacturers are required to use at least one appropriate test method to verify the identity of any component or excipient that is used in the manufacturing of a dietary supplement, as well as confirmation of identity of the product.
Simple sample preparation for the determination of vitamin D in serum by LC-MSMS
Tecan Group Ltd.

A growing number of laboratories have moved from the use of immunoassay methods to liquid chromatography coupled with tandem mass spectrometry (LC-MSMS) for the determination of vitamin D. The advantage of the latter method is unparalleled specificity combined with high sensitivity, offering the potential for the determination of multiple analytes, such as 25OH-vitamin D3 and 25OH-vitamin D2.
Automated Sample Preparation of 25-OH-Vitamin D3/D2 from Serum with the AC Extraction Plate and the Tecan Freedom EVO® for the quantification by LC-MS/MS
Tecan Group Ltd.

There is an increasing demand for the determination of Vitamin D status which is based on the quantification of the two metabolites 25-OH-Vitamin D3/D2. Numerous laboratories have moved from the use of immunoassay methods to liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) because of its specificity combined with high sensitivity. Moreover LC-MS/MS offers the potential for multianalyte determination which is required for Vitamin D status determination.
Colony Picking - fully automated screening of fungi
Tecan Group Ltd.

Manual screening processes are very costly and time consuming. This ambitious project for high throughput screening (HTS) of cellulase-producing fungal strains demonstrates the high capacity and unique features of the Freedom EVO colony picking workstation. The picking of fungi is more complex than for typical bacterial colonies, and requires advanced selection criteria to be combined with complex pipetting and a tip movement that mimics the manual picking process.
Can LC-MS/MS Be Used in Horse Meat Detection?
SCIEX

Following the Food Standards Agency’s announcement that horse and pig DNA had been identified in beef products sold by several supermarket chains, further testing has revealed widespread incidences of such contamination. This application note details a rapid, robust, sensitive and specific LC-MS/MS assay for the simultaneous detection of horse meat and the banned substance phenylbutazone (BUTE).
Determination of sodium in margarine manufacture
Metrohm AG

This application note describes the determination of the total sodium content of precursor solutions used in margarine manufacture.
Determination of sodium in soy milk
Metrohm AG

This Application Note describes the determination of the total Sodium content in soy milk products. The methodology may also be applied to the determination of Sodium in milk from cows, goats, and sheep.
SELECTBIO

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