Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

All-Enzymatic HPLC Method for Determination of Individual and Total Contents of Vitamin B6 in Foods

Published: Wednesday, June 13, 2012
Last Updated: Wednesday, June 13, 2012
Bookmark and Share
The study aimed to develop a novel method, which uses enzymatic reactions in all the steps and is applicable for determination of the seven individual vitamin B6 compounds in foods.

Background
There is a need for a reliable and accurate method for quantification of each of the seven individual vitamin B6 compounds including pyridoxine-β-glucoside in foods.

Objective
To determine pyridoxal (PL), pyridoxamine (PM), pyridoxine (PN), pyridoxal 5′-phosphate (PLP), pyridoxamine 5′-phosphate (PMP), pyridoxine 5′-phosphate (PNP), and pyridoxine-β-glucoside (PNG) in foods.

Design
By specific enzymatic treatment, each of the seven vitamin B6 compounds was all converted into 4-pyridoxolactone, which is a highly fluorescent compound. In total, seven separate, enzymatic steps were performed for each sample. Separation and quantification were performed with reversed-phase high performance liquid chromatography (HPLC) coupled with fluorescence detection. For each sample type the result was corrected for the recovery based on spiked samples. The method was applied for analyses of chicken liver, chicken white meat, egg yolk, egg white, dried anchovy, carrots, and garlic.

Results
The recovery varied from 14 to 114% in chicken liver, chicken white meat, egg yolk, egg white, dried anchovy, carrot, and garlic. Each food showed a characteristic distribution of the seven vitamin B6 compounds. The PNG was only found in low amounts; that is, 17–29nmol vitamin B6/g in the plant-derived foods, carrot and garlic. Only egg white showed a lower content, 3nmol/g. Overall the content in chicken liver, chicken white meat, and egg yolk had a total content of vitamin B6 between 42 and 51nmol/g. Both PM and PMP were high in the chicken liver. In contrast, PL and PLP were high in the chicken white meat. The main vitamin B6 in the egg yolk was PLP. The dried anchovy contained high amounts of PLP and PMP and a total content of 144nmol/g.

Conclusions
The enzymatic-based HPLC method was applied for the determination of seven vitamin B6 compounds in foods. Their distribution in the foods varied significantly.

The article is published online in Food & Nutrition Research and is free to access.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,400+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Acetaldehyde and Formaldehyde Content in Foods
Korean researchers have determined the content of the toxic and carcinogenic aldehydes, acetaldehyde and formaldehyde, in a variety of food groups.
Monday, November 09, 2015
Comparison of Caffeinated and Decaffeinated Coffee - Implications for Alzheimer's Disease
Researchers from the National University of Singapore successfully demonstrated the viability of GC-TOF-MS as an analytical platform for metabolomics analysis of coffee samples.
Monday, September 08, 2014
Immunochromatographic Assay for Rapid Salmonella Detection
The test strip described in the study would be beneficial for poultry farms and other veterinary production for the monitoring of S. Typhimurium and/or S. Enteritidis contamination.
Tuesday, August 12, 2014
Application of MALDI-TOF MS for the Identification of Food Borne Bacteria
MALDI-TOF MS is a promising platform for quick, flexible, and reliable identification of food microbial isolates.
Tuesday, April 29, 2014
Monitoring and Risk Assessment of Pesticide Residues in Commercially Dried Vegetables
The study aimed to analyze the presence of 253 pesticides commonly used on dried vegetables and to check their compliance with existing regulations.
Tuesday, March 11, 2014
Determination of Cyanogenic Compounds in Edible Plants by Ion Chromatography
Ion chromatography following acid hydrolysis was employed to prevent thiocyanate interference of the colorimetric methods.
Tuesday, December 10, 2013
LC-MS/MS-Based Multibiomarker Approaches for the Assessment of Human Exposure to Mycotoxins
To overcome the disadvantages of indirect methods, biomarker approaches were developed as a biomonitoring tool for some major mycotoxins.
Wednesday, November 13, 2013
Predictors of Obesity: The "Power" of the Omics
Diet and nutrient biomarkers should provide objective measures of dietary intake and nutritional status, as well as an integrated measure of intake, absorption and metabolism.
Friday, October 25, 2013
Optical Biosensors for Food Quality and Safety Assurance
With high specificity, convenience and quick response there is growing interest in biosensors for food safety testing.
Monday, October 07, 2013
Pesticide Residue Screening Using a Novel Artificial Neural Network Combined with a Bioelectric Cellular Biosensor
Researchers have developed and trained a customized feed forward artificial neural network for the classification of the pesticide groups, pyrethroids, carbamates, and organophosphates.
Friday, September 20, 2013
Effect of Trans Fatty Acid Intake on LC-MS and NMR Plasma Profiles
In this study, NMR and LC-MS untargeted metabolomics has been used as an approach to explore the impact of trans fatty acid intake on plasma metabolites.
Tuesday, August 13, 2013
Low Density Solvent-Based Dispersive Liquid-Liquid Microextraction for the Determination of Synthetic Antioxidants in Beverages by HPLC
In this study, researchers propose a method that could be used for monitoring as well as controlling the synthetic antioxidants in aqueous food samples.
Wednesday, July 31, 2013
What's New in the Diagnosis and Management of Food Allergy in Children?
With the increasing worldwide prevalence of food allergy comes a renewed impetus for improving diagnosis and management.
Monday, June 24, 2013
Genetically Modified Crops and Food Security
Although GM crops are not a panacea for the problems of hunger and malnutrition, evidence suggests that GM crops can be an important component in a broader food security strategy.
Monday, June 24, 2013
A Simple HPLC Method for Determining the Purine Content of Beer and Beer-Like Alcoholic Beverages
In this article researchers from Teikyo University report an improved method for determining small amounts of purines in alcoholic beverages.
Wednesday, May 29, 2013
Scientific News
Apricot Kernels Pose Risk of Cyanide Poisoning
Eating more than three small raw apricot kernels, or less than half of one large kernel, in a serving can exceed safe levels. Toddlers consuming even one small apricot kernel risk being over the safe level.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
First DNA Vaccine in the EU Recommended for Use in Salmon
Clynav to protect Atlantic salmon from serious infectious disease.
Grant to Fund Million Peaks Project
The European Research Council (ERC) has awarded a prestigious Advanced Grant to Prof. Peter Schoenmakers, Prof. Albert Polman and Prof. Huib Bakker, all three of whom work at the University of Amsterdam (UvA).
Finding Inorganic Arsenic in Foodstuffs
A new European standard method to determine the content of inorganic arsenic in foodstuffs has been developed at the National Food Institute, Technical University of Denmark.
Effective Identification of Low-Gliadin Wheat Lines
Researchers have demonstrated the use of NIRS to identify low-gliadin wheat lines.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Interactive Maps Reveal Global Obesity
World’s obese population hits 640 million, according to largest ever study.
Eating Green Could be in Your Genes
Genetic variation uncovered that has evolved in populations that have historically favored vegetarian diets, such as in India, Africa and parts of East Asia.
Detecting Bacterial Growth in Packaged Food
New technique enables fast, accurate and noninvasive measurement of bacteria levels.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!