Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

AB SCIEX Improves Food Safety with New Method to Identify Markers for Horse Meat

Published: Friday, May 24, 2013
Last Updated: Friday, May 24, 2013
Bookmark and Share
New LC/MS/MS-based meat speciation method detects numerous animal protein markers in a single analysis with higher accuracy and reliability than existing methods.

AB SCIEX announced that its scientists have developed a new method for detecting horse tissue present in meat samples. This is in response to recent reports, including the Food Standards Agency’s announcement earlier this year, that horse and pig DNA had been identified in beef products sold in several supermarket chains in Europe.

The new AB SCIEX method, which is based on LC/MS/MS (liquid chromatography / tandem mass spectrometry), represents a novel, more accurate approach to meat speciation. It detects the protein markers distinct to specific meat species and confirming the presence of a particular species in a sample by direct detection. The method also enables laboratories to detect veterinary drug residues in the same analysis.

While the method was optimized to identify horse tissue contamination in beef samples, it may also be adapted to detect peptide markers of numerous different animal types simultaneously. Ultimately, the method has the power to identify horse meat that may be present in other tissue samples (such as pork, beef or lamb) by its unique protein markers.

“Our new method shows a clear role for LC/MS/MS in meat speciation, giving scientists the most reliable results for identification of horse meat in food,” said Vincent Paez, Sr. Director for Food and Environmental at AB SCIEX. “It further demonstrates our commitment to helping companies and governments improve food safety and verify food authenticity.”

The horse meat markers that AB SCIEX scientists identified have been independently verified by research scientists at the University of Münster (Prof. Dr. Hans-Ulrich Humpf, Dr. Jens Brockmeyer and Christoph von Bargen). In addition, AB SCIEX scientists used the method in a recent LGC Standards proficiency testing scheme, confirming that the method can easily detect horse meat in beef at levels of 10% with no false positives. The method was then adapted to detect horse meat at levels as low as 1% in beef samples, making the method detection limits comparable to those achieved with existing methodologies such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) techniques.

The AB SCIEX mass spectrometry-based method offers a more accurate and reliable approach to meat speciation than PCR, which must detect the animal’s DNA, or ELISA, which must detect the intact animal proteins ‒ both of which have limitations. Additionally, this method is an improvement over other indirect LC/MS/MS-based methods, which target veterinary drug residues rather than the targeted animal proteins themselves. This new approach, however, does allow for the detection of veterinary drug residues in the same analysis, which is not possible by ELISA or PCR.

The new method was developed on an AB SCIEX LC/MS/MS platform, consisting of an AB SCIEX QTRAP® 5500 LC/MS/MS system coupled with an Eksigentekspert™ micro LC system. The QTRAP® 5500 system uses multiple reaction monitoring (MRM) to detect each peptide and is then capable of providing sequence information by acquiring a product ion scan for each triggered MRM, which can be used to confirm the peptide’s identity. This gives greater confidence for food testing when distinguishing between species. This is especially significant because, for example, horse and beef proteins may differ by as little as one or two amino acids.

“LC/MS/MS will allow laboratory scientists to analyse a single meat sample for 15-20 different animal species in a single run, with very little chance of false positives,” said Stephen Lock, PhD, Technical Market Applications Manager, Food & Environmental, AB SCIEX. “Future work is planned to add more markers to the method and further increase the number of species detected with this approach.”

As a leader in next-generation food testing technologies, AB SCIEX has previously developed similar methods for protein screening in food, including new techniques for detecting allergens such as eggs, milk, sesame seeds, nuts, and mustard simultaneously in food samples, as well as more recent work investigating casein detection in wine.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Improved Accuracy of Pesticide Detection in Food
European Reference Laboratory improves accuracy of pesticide detection in food with AB SCIEX mass spectrometry instrumentation.
Wednesday, February 04, 2015
AB SCIEX Wins New Product Innovation Award in Asia Pacific
The award recognizes the company’s continued innovation in the mass spectrometry industry and a number of new products introduced into the market in recent years.
Thursday, October 30, 2014
Halal Meat Authenticity Testing Reassures Consumers of No Pig or Horse Contamination
New LC/MS/MS-based meat speciation method allows food testing laboratories to detect pig and horse contamination in diverse food products, with higher accuracy than existing methods.
Tuesday, January 28, 2014
AB SCIEX Brings MASStastic Voyage to Ireland
The tour features a state-of-the-art mobile laboratory and will coincide with a special conference being held by the Irish Separation Science Cluster (ISSC).
Monday, November 11, 2013
AB SCIEX Expands in Singapore
AB SCIEX announce the next major phase of its expansion in Singapore, with the grand opening of a new R&D centre.
Friday, November 01, 2013
AB SCIEX Expands Support in the Middle East
AB SCIEX announces the opening of a new regional office and technical support centre located in Dubai.
Tuesday, October 29, 2013
AB SCIEX Receives Frost & Sullivan Award
AB SCIEX has been awarded the 2013 Global Market Share Leadership Award for Mass Spectrometry from Frost & Sullivan.
Thursday, September 12, 2013
Eurofins Collaborates with AB SCIEX and Phenomenex
Accelerating the development of new methods available to the food testing community worldwide.
Thursday, July 04, 2013
AB SCIEX Responds to Milk Contamination Concerns with New Method to Detect DCD
Easy-to-use method equips food and beverage testing laboratories around the world for rapid response.
Wednesday, February 13, 2013
Mobile Laboratory on European MASStastic Voyage Tour to Roll in 2013
Free breakfast seminar on board at each stop to showcase scientific technologies for a wide variety of applications in Europe.
Monday, January 28, 2013
AB Sciex Agrees for Sigma-Aldrich® to Distribute iChemistry™ Solutions
Broadens availability of mass spectrometry-based tagging chemistries to biologists and chemists worldwide.
Wednesday, September 12, 2012
AB SCIEX Expands Commercial Operations in Brazil
The expansion will support the increasing use of mass spectrometry for food safety testing, environmental analysis, academic research, drug development, forensic toxicology and clinical research.
Monday, March 19, 2012
AB SCIEX Opens New Asia Pacific Application and Training Centre
The centre is located in Singapore’s biomedical hub at Biopolis and provides comprehensive service, support and application development.
Monday, March 12, 2012
AB SCIEX and Phenomenex Mobile Laboratory to address Food Safety, Clinical Research, Forensics and the Environment
The North American tour will commence at Pittcon 2012 to demonstrate next-generation LC/MS/MS workflows.
Thursday, February 16, 2012
AB SCIEX and Phenomenex Respond to Orange Juice Contamination Concerns
New method is in response to recent governmental concerns that shipments from Brazil were tainted with the fungicide.
Tuesday, February 14, 2012
Scientific News
Study Questions Presence in Blood of Heart-Healthy Molecules from Fish Oil Supplements
A new study from the Perelman School of Medicine at the University of Pennsylvania questions the relevance of fish oil-derived SPMs and their purported anti-inflammatory effects in humans.
How To Keep Your Rice Arsenic-Free
Researchers at Queen’s University Belfast have made a breakthrough in discovering how to lower worrying levels of arsenic in rice that is eaten all over the world.
Pesticide Found in 70 Percent of Massachusetts’ Honey Samples
New Harvard University study says that the pesticide commonly found in honey samples is implicated in Colony Collapse Disorder.
Printed "Smart Cap" Detects Spoiled Food
It might not be long before consumers can just hit “print” to create an electronic circuit or wireless sensor in the comfort of their homes.
Red Wine Antioxidant May Provide New Cancer Therapy Options
Resveratrol and quercetin, two polyphenols that have been widely studied for their health properties, may soon become the basis of an important new advance in cancer treatment,
New Research will Show How the Environment Could Change the Way We Eat
A new study funded by the Wellcome Trust will investigate how environmental changes over the next 20-30 years may impact the way we eat, in the UK and worldwide.
Blue LEDs Can be Used to Preserve Food
Blue light emitting diodes (LEDs) have strong antibacterial effect on major foodborne pathogens and can be used as a chemical-free food preservation method, a new study has found.
FDA Declares Trans Fatty Acids Unsafe for Consumption
TFAs are widely recognized as the most harmful fat with regard to causing cardiovascular disease (CVD).
Fat, Sugar Cause Bacterial Changes that may Relate to Loss of Cognitive Function
A study has indicated that both a high-fat and a high-sugar diet, compared to a normal diet, cause changes in gut bacteria that appear related to a significant loss of "cognitive flexibility," or the power to adapt and adjust to changing situations.
How Anthrax Spores Grow in Cultured Human Tissues
New findings to help predict risk and outcomes of anthrax attacks.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!