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Measurement of Phenolic Compounds in Red Wines Using the SpectraMax® Plus384 Microplate Reader
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Molecular Devices

Measurement of tannin, iron-reactive phenolics, anthocyanin and polymeric pigment in red wine is an important part of quality control in the wine industry. Precise and reliable measurement of phenolic compounds in wine is critical for making decisions during fermentation, maceration, pressing and blending. Harbertson et al. developed a comprehensive red wine phenolics assay in 2003.1 Traditionally this assay is performed using a cuvette-based UV-vis spectrophotometer. It is a time-consuming and laborious process of reading individual samples in separate cuvettes, recording the results, and analyzing the data. Identifying the need for a rapid and cost-effective way of performing the assay, Heredia et al. adapted the assay for a microplate platform. This approach increases the throughput with respect to labor, time and sample volume. The assay widens the capacity to monitor the fermentations for extraction of phenolic compounds in an average-sized winery. Here we describe the use of MDS Analytical Technologies’ SpectraMax® Plus384 microplate reader and SoftMax® Pro software to efficiently collect and analyze the data for this assay.

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