Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Process Contaminants in Vegetable Oils and Foods
Glycerol-based process contaminants found in palm oil, but also in other vegetable oils, margarines and some processed foods, raise potential health concerns for average consumers of these foods in all young age groups, and for high consumers in all age groups.
Apricot Kernels Pose Risk of Cyanide Poisoning
Eating more than three small raw apricot kernels, or less than half of one large kernel, in a serving can exceed safe levels. Toddlers consuming even one small apricot kernel risk being over the safe level.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
First DNA Vaccine in the EU Recommended for Use in Salmon
Clynav to protect Atlantic salmon from serious infectious disease.
Grant to Fund Million Peaks Project
The European Research Council (ERC) has awarded a prestigious Advanced Grant to Prof. Peter Schoenmakers, Prof. Albert Polman and Prof. Huib Bakker, all three of whom work at the University of Amsterdam (UvA).
Finding Inorganic Arsenic in Foodstuffs
A new European standard method to determine the content of inorganic arsenic in foodstuffs has been developed at the National Food Institute, Technical University of Denmark.
Effective Identification of Low-Gliadin Wheat Lines
Researchers have demonstrated the use of NIRS to identify low-gliadin wheat lines.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Interactive Maps Reveal Global Obesity
World’s obese population hits 640 million, according to largest ever study.
Eating Green Could be in Your Genes
Genetic variation uncovered that has evolved in populations that have historically favored vegetarian diets, such as in India, Africa and parts of East Asia.
Scroll Up
Scroll Down

Two-Dimensional HPLC Combined with On-Line SPE for Determination of Sudan Dyes I–IV in Chili Oil
Bookmark and Share

Dionex - Part of Thermo Fisher Scientific

The determination of Sudan dyes in complex matrices (e.g., chili oil) often requires extensive sample preparation prior to HPLC analysis. Due to the fat-solubility of Sudan dyes, extraction with organic solvents such as acetonitrile, methanol, n-hexane, cyclohexane, and petroleum ether is typically used. Some supplementary means (e.g., ultrasound-assisted and microwaveassisted extractions) are applied to improve the extraction efficiency. The procedure following extraction is cleanup, which is the bottleneck for the sensitive determination of Sudan dyes in chili oil. Solid-phase extraction (SPE), gel permeation chromatography, thin-layer chromatography and dispersive solid-phase extraction have been reported for cleanup.

Here, a two-dimensional HPLC with on-line SPE intercolumn trapping method was developed for fast, effective determination of Sudan dyes I–IV in chili oil. Following extraction with methylene dichloride and acetonitrile, the analytes are separated in the first dimension and removed selectively to be absorbed on an SPE column. This eliminates numerous matrix interferences. When analyte trapping is complete on the SPE column, it is switched into the flow path of the second dimension, where the absorbed dyes are eluted from the SPE column, separated, and detected. The 2-D HPLC with on-line SPE intercolumn trapping system runs automatically on the Thermo Scientific Dionex UltiMate™ 3000 HPLC dual-pump system controlled by Thermo Scientific Dionex Chromeleon™ software, and provides the advantages of full automation, absence of operator influence, and strict process control compared to a typical off-line SPE method.

Further Information


SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!