Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Apricot Kernels Pose Risk of Cyanide Poisoning
Eating more than three small raw apricot kernels, or less than half of one large kernel, in a serving can exceed safe levels. Toddlers consuming even one small apricot kernel risk being over the safe level.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
First DNA Vaccine in the EU Recommended for Use in Salmon
Clynav to protect Atlantic salmon from serious infectious disease.
Grant to Fund Million Peaks Project
The European Research Council (ERC) has awarded a prestigious Advanced Grant to Prof. Peter Schoenmakers, Prof. Albert Polman and Prof. Huib Bakker, all three of whom work at the University of Amsterdam (UvA).
Finding Inorganic Arsenic in Foodstuffs
A new European standard method to determine the content of inorganic arsenic in foodstuffs has been developed at the National Food Institute, Technical University of Denmark.
Effective Identification of Low-Gliadin Wheat Lines
Researchers have demonstrated the use of NIRS to identify low-gliadin wheat lines.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Interactive Maps Reveal Global Obesity
World’s obese population hits 640 million, according to largest ever study.
Eating Green Could be in Your Genes
Genetic variation uncovered that has evolved in populations that have historically favored vegetarian diets, such as in India, Africa and parts of East Asia.
Detecting Bacterial Growth in Packaged Food
New technique enables fast, accurate and noninvasive measurement of bacteria levels.
Scroll Up
Scroll Down

Determination of Antioxidant potential using an Oxygen Radical Absorbance Capacity (ORAC) Assay with Synergy™ H4
Bookmark and Share

BioTek Instruments

Oxidative damage to living organisms has been associated with several disease states as well as aging. The sources of reactive oxygen species (ROS) are varied and can lead to the formation of toxic compounds within organisms. ROS sources such as cellular respiration, interaction of biomolecules with ionizing radiation, and dedicated cellular pathways for ROS formation as a protective mechanism ensure chronic exposure of living organisms to ROS species.

A proper balance must be maintained between oxidants and antioxidants to ensure the ubiquitous ROS species do not become deleterious to the organism. Disruption of any one of these mechanisms can result in an imbalance and ensuing damage to a number of critical components required to maintain cellular homeostasis. Thus, there is interest in the ability to accurately determine the antioxidant capabilities of foods, cosmetics, dietary supplements and pharmaceutical agents.

While several methods exist to measure total antioxidant capacity, ORAC has emerged as a low cost method suitable for high throughput automation in a microplate format. The ORAC assay relies on free radical damage to a fluorescent probe, most commonly fluorescein, caused by an oxidizing reagent resulting in a loss of fluorescent intensity over time.The resultant damage can then be correlated with the amount of oxidant present. Conversely, inhibition of oxidative damage to the fluorescent probe can be correlated with the antioxidant capacity of a compound acting as a free radical scavenger.

This application note demonstrates the ability to perform the assay in a 96-well microplate format amenable to higher throughput platforms.

Further Information


SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!