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  Events - December 2012


3 days Hands-on qPCR

10 Dec 2012 - 12 Dec 2012 - TATAA Biocenter, Prague



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3 DAYS HANDS-ON QPCR

Target audience: Beginners to Medium experienced qPCR users

Entrance qualifications: Basic Molecular biology or similar

Description: The basic real-time qPCR course. You will aquire a comprehensive overview of the possibilities with real-time PCR, how to use it and how to analyze the results. The course contains:

Day 1

Basic PCR theory
The theory of real-time PCR
Applications and possibilities of qPCR. Comparison of qPCR with regular PCR.
Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons, etc)
Different instrument platforms and their typical uses
Primer design
The problem of primer-dimer formation and how to minimize them
Probe design
Experimental design and optimization
Basic data handling and analysis

Day 2

Introduction to quantification principles
Quantification strategies, uses and limitations
Calculations using different relative quantification methods
Strategies for normalization of qPCR data
In situ calibration for compensation of inhibition in samples
Absolute quantification

Day 3

Sample Preparation (Extraction of RNA and DNA)
Principles of RT
Priming methods for RT
RT optimization
The MIQE guidelines
Group discussion, bring your own questions



Further information
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Cell biologists have reported an advance in understanding the workings of an error correction mechanism that helps cells detect and correct mistakes in cell division early enough to prevent chromosome mis-segregation and aneuploidy, that is, having too many or too few chromosomes.
How to Become a Follicular T Helper Cell
Uncovering the signals that govern the fate of T helper cells is a big step toward improved vaccine design.
Researchers Resurrect Ancient Viruses
Researchers at Massachusetts Eye and Ear and Schepens Eye Research Institute have reconstructed an ancient virus that is highly effective at delivering gene therapies to the liver, muscle, and retina.
Cell Aging Slowed by Putting Brakes on Noisy Transcription
Experiments in yeast hint at ways to extend life of some human cells.
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
Expanding the Brain
A team of researchers has identified more than 40 new “imprinted” genes, in which either the maternal or paternal copy of a gene is expressed while the other is silenced.
Identifying a Key Growth Factor in Cell Proliferation
Researchers discover that aspartate is a limiter of cell proliferation.
Study Uncovers Target for Preventing Huntington’s Disease
Scientists from Cardiff University believe that a treatment to prevent or delay the symptoms of Huntington’s disease could now be much closer, following a major breakthrough.
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