Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Powerful Walk Away qPCR Set-up from Tecan

Published: Monday, July 23, 2012
Last Updated: Monday, July 23, 2012
Bookmark and Share
Freedom EVO® 150 with LightCycler® 480 real-time PCR system to investigate the biology of viral cancers.

The Dittmer Lab at the University of North Carolina is using a Freedom EVO® 150 with an integrated LightCycler® 480 Real-Time PCR System to perform research activities and investigate the biology of viral cancers, such as those caused by Kaposi’s sarcoma-associated herpesvirus (KSHV).

The Group uses qPCR profiling to study various aspects of KSHV, as postdoctoral research associate Pauline Chugh explained: “We use three main strategies to investigate viral tumorigenesis - viral gene expression, microRNA (miRNA) expression and viral load testing of clinical samples - based on 96 primer qPCR arrays. Performing the assays manually would be very labor-intensive, and so we use automation to provide the throughput necessary for these studies.”

The Group’s Freedom EVO system provides walk away automation of sample preparation for subsequent qualitative and quantitative nucleic acid detection by real-time PCR, ensuring accurate and reliable assay plate setup in a 384 well format.

Pauline continued: “The workstation gives us the ability to perform automated analysis of up to 750 gene sequences per sample in just one day, allowing us to, for example, look at the whole miRNA library following infection with the virus. We have also been able to miniaturize many of our protocols, which offers significant cost savings in terms of reagents. Having the ability to multitask has also significantly improved our productivity, allowing us to perform other experiments or analyze data while the instrument is running. Alternatively, we can load 60 individual arrays onto the Freedom EVO and leave the system running overnight. It’s basically generating data while you sleep.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
A Diversity of Genomes
New DNA from understudied groups reveals modern genetic variation, ancient population shifts.
Gene Could Reduce Female Mosquitoes
Virginia Tech researchers have found a gene that can reduce female mosquitoes over many generations.
Improving Crop Efficiency with CRISPR
New study of CRISPR-Cas9 technology from Virginia Tech shows potential to improve crop efficiency.
Examining mtDNA May Help Identify Unknown Ancestry That Influences Breast Cancer Risk
Researchers studying mtDNA in a group of triple negative breast cancer patients found that 13 percent of participants were unaware of ancestry that could influence their risk of cancer.
Bacteria Use Ranking Strategy to Fight Off Viruses
Researchers have explained why microbes store virus confrontation information sequentially, with most recent attacks first.
Gene Therapy Technique May Help Prevent Cancer Metastasis
Gene-regulating RNA molecules could help treat early-stage breast cancer tumors before they spread.
Enhancing Antibiotics to Defeat Resistant Bacteria
Scientists enhance ability of antibiotics to defeat resistant types of bacteria using molecules called PPMOs
The Genetics of Blood Pressure
Researchers have identifed areas of the genome associated with blood-pressure including 17 previously unknown loci.
Mosquito Genetics Determine Tastes
Study reveals mosuito's preference for human versus animal biting is determined by genetics.
Quadruple Helix DNA Aids Cancer Therapies
Researchers have identified the role that a four-stranded version of DNA may play in the role of cancer progression.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!