Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

Bioo Scientific Launches the NEXTflex Bisulfite-Seq Kit for NGS Methylome Analysis

Published: Tuesday, August 14, 2012
Last Updated: Tuesday, August 14, 2012
Bookmark and Share
The NEXTflex™ Bisulfite-Seq Kit from Bioo Scientific is the first complete kit available designed to simplify library preparation of bisulfite treated DNA for down-stream high-throughput sequencing.

Bisulfite-seq.gifBisulfite-seq is an important method for studying DNA methylation and epigenetic gene regulation as it allows for an unbiased genome-wide analysis of DNA methylation generating a high-accuracy methylome.

NEXTflex™ Bisulfite-Seq Kit is designed to work with reduced representation or total bisulfite-converted DNA libraries and is compatible with single, paired-end and multiplexed DNA libraries on Illumina® MiSeq, GAIIx and HiSeq platforms. It includes Bioo Scientific’s proprietary "Enhanced Adapter Ligation Technology” which offers improved ligation efficiency than other commercially available solutions resulting in library preps with a larger number of unique sequencing reads. This kit also uses a completely gel-free protocol making the workflow compatible with liquid handler automation. For multiplexing, NEXTflex™ Bisulfite-Seq Barcodes can be purchased separately.

Bioo Scientific has also launched the NEXTflex™ Msp1 Restriction Enzyme, which unlike other commercially available Msp1 restriction enzymes, has been optimized for bisulfite sequencing.

According to Dr. Masoud Toloue, Director of Genomic Research at Bioo Scientific, “Short of direct sequencing of methylated cytosines, Bisulfite-Seq is currently the most sensitive and accurate method of elucidating epigenetic marks, the NEXTflex kit is designed to get you there.”

The NEXTflex™ Bisulfite-Seq Kit and Barcodes are the latest addition to Bioo Scientific’s line of innovative kits for next generation sequencing library preparation and, like our other library prep kits, the NEXTflex Bisulfite-Seq Kits are all available for next day delivery.

Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,200+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Bioo Scientific Awarded $750,000 NSF Grant
Company awarded a National Science Foundation Phase II grant to continue development efforts to improve the accuracy of small RNA expression analysis.
Wednesday, November 26, 2014
Bioo Scientific Receives Presidential Award for Exports
Awards are the highest recognition any U.S. entity may receive for making a significant contribution to the expansion of U.S. exports.
Thursday, May 29, 2014
NSF Funds Research Partnership Between Bioo Scientific and Mirna Therapeutics
The funding will support tumor regression and pharmacokinetic studies on targeted delivery of microRNAs.
Thursday, July 22, 2010
Bioo Scientific Awarded SBIR Grant for Development of Targeted RNAi In Vivo Delivery Technology
A $500,000 Phase II grant will help to develop kits and reagents based on the patent-pending Targeted Transport Technology.
Wednesday, July 29, 2009
Bioo Scientific Corp. Receives NIH Grant for the Development of Novel Anti-Viral Therapeutics
Researchers discover a mechanism by which several viruses suppress aspects of the cell to stabilize their nucleic acids to promote an efficient infection.
Tuesday, May 05, 2009
Custom RNA Interference Service
BIOO Scientific is launching a service to perform in vivo RNAi testing to facilitate progress in the drug pipeline.
Thursday, April 12, 2007
Scientific News
Integrated Omics Analysis
Studying multi-omics promises to give a more holistic picture of the organism and its place in its ecosystem, however despite the complexities involved those within the field are optimistic.
Unravelling the Role of Key Genes and DNA Methylation in Blood Cell Malignancies
Researchers from the University of Nebraska Medical Center have demonstrated the role of Dnmt3a in safeguarding normal haematopoiesis.
Zika Virus Infection Alters Human and Viral RNA
Researchers have discovered that Zika infections results in human and viral genetic modification.
Cell Metabolism Linked to Spread of Cancer
Scientists discover macrophage metabolism can be attuned to prevent the spread of cancer.
Agilent Presents Early Career Professor Award to Dr. Roeland Verhaak
JAX professor recognized for the development and implementation of workflows for the analysis of big-data from transcriptomics to next generation sequencing approaches.
Bacterial Genes Boost Current in Human Cells
Borrowing and tweaking bacterial genes to enhance electrical activity might treat heart, nervous system injury.
Ovarian Cancer Insight
Study showed tumours release cytokines to attract macrophages, which secrete growth factors that in turn promote tumour growth.
Questioning the Safety of Selenium to Combat Cancer
Research indicates the need for change in practice as selenium supplements cannot be recommended for preventing colorectal cancer.
Less Frequent Cervical Cancer Screening
HPV-vaccinated women may only need one screening every 5 to 10 years with screening starting later in life.
Supercomputers Could Improve Cancer Diagnostics
Researchers push the boundaries of cancer research through high-performance computing to map the human immunone.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,200+ scientific videos