Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

CYTOO’s 2D+ Cell Culture Platform Reproduces in vivo Conditions to Study Tumor Cell Motility

Published: Thursday, October 18, 2012
Last Updated: Thursday, October 18, 2012
Bookmark and Share
New perspectives in oncology, genetics and drug screening.

CYTOO S.A. announced new results that demonstrate the ability of the Company’s 2D+ Cell Culture Platform to reproduce in vivo conditions to analyze tumor cell motility and in particular to study fibrillar ECM-dependent tumor cell-macrophage pairing and migration involved in tumor metastasis. These results have recently been published in the first issue of the journal IntraVital, edited by Landes Bioscience.

The CYTOO 2D+ Cell Culture Platform is based on the use of adhesive micropatterns to guide cell architecture and behavior in culture, in contrast to current 2D cell culture where cells spread and move in an uncontrolled manner. By defining the 2D topology of cell adhesion, 2D+ Technology enables the fine control of the spreading and 3D shape of cultured cells in single- or multi-cellular configurations resulting in control of cell contractility, cell polarity, organelle positioning, or cell division axis.

Researchers Ved Sharma, Brian Beaty, Antonia Patsialou, Dianne Cox, John Condeelis and Robert Eddy from the Albert Einstein College of Medicine, NY, with collaborators Huiping Liu from University of Chicago and Michael Clarke at Stamford School of Medicine, used CYTOOchipsTM Motility to reconstitute an in vitro model of fibrillar tumor extracellular matrix (ECM). The micropatterned 1D adhesive tracks were used to mimic the linear ECM fibers of the tumor microenvironment.

Similar morphologies, behaviors and motility rates were observed in vivo and on micropatterned lines. In particular, tumor cell velocity on 1D substrates was in agreement with the high velocity values of tumor cells on ECM fibers observed in vivo. In contrast, on classical 2D substrates, motility rates were ten fold lower than what can be observed in vivo. On micropatterned lines, the authors could also reproduce the assembly of alternating tumor cells and macrophages identified as streams in vivo, the ability of macrophages to enhance protrusion velocity and average velocity of tumor cells and showed that this effect was dependent on an intact paracrine loop without any additional need of co-factors.

The authors concluded that their “1D micropatterned substrate model more closely approximates the fibrillar nature of the in vivo tumor microenvironment and offers a simple and more appropriate substrate for detailed analyses of cell protrusion, cell-cell pairing and migration than conventional 2D substrates. The data presented here validates the use of micropatterned 1D adhesive substrates to study the fibrillar ECM found within the tumor microenvironment.”

Co-author Robert Eddy commented “It was a surprise that tumor cell and macrophage streaming behavior we observe in the highly complex tumor microenvironment was self-organizing and required no other extracellular cues on 1D adhesive substrates.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Breast Cancer Cells Found To Switch Molecular Characteristics
Spontaneous interconversion between HER2-positive and HER2-negative states could contribute to progression, treatment resistance in breast cancer.
Some Breast Cancer Patients With Low Genetic Risk Could Skip Chemotherapy
Genetic test can help predict survival and guide treatment options.
Lose Weight, Escape the Eight: Weight-Based Cancer Risk
IARC has identified eight additional cancer sites linked to overweight and obesity.
Coffee Consumption Linked to Genes
Researchers have identified a gene that influences coffee consumption. The gene is thought to relate to caffeine breakdown.
Emerging Model of Cancer
Cancer acts cooperatively, making individual decisions but acting in unison; this insight is being used to create a computer model of cancer.
Biological Barcodes Using CRISPR
Using genome editing tools, researchers are getting closer to understand differentiation of various cell types during development.
Controlling DNA Repair
Scientists discover that DNA repair outcomes following CRISPR-Cas9 cleaving are non-random and can be harnessed to produce desired effects.
Demonstrating LNP Delivery of CRISPR Components
Intellia has presented data demonstrating in vivo gene editing ising liquid nanoparticles (LNPs) to deliver CRISPR/Cas9.
Gene Therapy Via Ultrasound
Research into a gene therapy approach called sonoporation could help combat heart disease and cancer.
Creating Embryos with 'Heteroplasmy'
New discovery in genetic research could lead to treatments for mitochondrial diseases.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!