Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Droplet Digital™ PCR Improves Detection Sensitivity of Telomerase Activity Assay

Published: Thursday, April 11, 2013
Last Updated: Thursday, April 11, 2013
Bookmark and Share
New research shows that Bio-Rad’s Droplet Digital PCR (ddPCR™) technology can dramatically improve the sensitivity, precision, and throughput of a popular assay for telomerase activity.

Researchers from the University of California, San Francisco, and Bio-Rad Laboratories, Inc. are using Bio-Rad’s ddPCR technology with intercalating dye chemistry to provide absolute quantification of telomerase activity.

Telomeres, the protective structures at the ends of chromosomes, naturally degrade with each cell division. Once they are below a critical length, the cells arrest and become senescent before dying or passing through crisis, incurring additional genomic mutations, and becoming immortalized cancer cells.

One of the mechanisms of immortality is the activation of the telomerase enzyme. This enzyme adds a specific sequence of nucleotide repeats to telomeres, thus rewinding the clock and enabling a cell to divide continuously. Researchers are investigating telomerase activity as a biomarker for cancer diagnosis and as a target for anticancer drugs. Measuring telomerase activity more sensitively may enhance our understanding of its role in oncogenesis and other cellular and physiological phenotypes.

ddPCR Technology Enhances the Telomerase Activity Assay 
Traditionally, the telomerase repeat amplification protocol (TRAP) assay measures the presence of active telomerase by measuring the activity of the enzyme on a starting DNA template, which is then amplified by PCR. For samples with abundant telomerase activity, SYBR® Green qPCR assays deliver high throughput and sufficient sensitivity. However, the most sensitive detection method still requires radioactive labeling, laborious polyacrylamide sequencing gels, and densitometry.

In the cited study, single-molecule counting of telomerase-extended templates was accomplished by partitioning the sample into droplets followed by PCR amplification and detection by fluorescence droplet reading using Bio-Rad’s ddPCR technology. The study results indicate that ddPCR is significantly more sensitive than traditional TRAP radiography and is also more amenable to high-throughput analysis.

“This new (ddPCR TRAP) telomerase activity assay demonstrates the ability to quantify telomerase enzymatic activity in droplets,” said George Karlin-Neumann, director of scientific affairs at Bio-Rad’s Digital Biology Center. “The sensitivity of ddPCR technology enables the measurement of telomerase activity in samples below the detection limit of traditional radiographic methods, while also providing a quick and facile method of measurement in cells where telomerase is more highly expressed.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Assessing the Effectiveness of Genome-Editing Technologies
Researchers have developed a cost-effective and rapid method for assessing edits generated by CRISPR-Cas9 and other genome-editing technologies.
Thursday, June 30, 2016
Awards for Bio-Rad's Protein Expression Products and Genomic Workflow Solutions
BioInformatics LLC has recognized Bio-Rad with two prestigious Life Science Industry Awards for “Best Protein Expression & Analysis Products” and “Best Workflow Solutions — Genomics.”
Monday, January 19, 2015
Droplet Digital PCR Enables Measurement of Potential Cancer Survival Biomarker
Study paves the way for further research into the role of TIL quantification in immunotherapy and as a cancer survival predictor.
Tuesday, December 10, 2013
A Brief RT-qPCR “Field Guide” for MIQE Adherence
Bio-Rad’s Sean Taylor and Eli Mrkusich have published a practical guide for MIQE compliance.
Monday, December 09, 2013
Accurate Detection of Extremely Rare Mitochondrial DNA Deletions Associated with Aging
The study published in Aging Cell identifies a new tool to accurately analyze extremely rare mitochondrial DNA deletions associated with a range of diseases and disorders as well as aging.
Thursday, September 05, 2013
Bio-Rad Announces Mini-Protean Tetra Cell Users Website
The new online social networking site is created for current and new Bio-Rad Mini-PROTEAN® system users.
Wednesday, August 20, 2008
Bio-Rad Launches new Website for Researchers Transfecting Mammalian Cells
Bio-Rad adds ‘Gene Transfer Protocols’ to its Gene Expression Gateway Web-site, dedicated to researchers working with mammalian cells.
Friday, June 27, 2008
Testing Results of the BioOdyssey Calligrapher MiniArrayer for Clinical Research Available
Bio-Rad announces the availability of a technical bulletin describing results of the testing of the BioOdyssey™ Calligrapher™ MiniArrayer.
Thursday, November 08, 2007
Scientific News
Faecal Bacteria Linked to Body Fat
Researchers at King’s College London have found a new link between the diversity of bacteria in human poo – known as the human faecal microbiome - and levels of abdominal body fat.
Scientists Find Lethal Vulnerability in Treatment-Resistant Lung Cancer
The study describes how the drug Selinexor killed lung cancer cells and shrank tumors in mice when used against cancers driven by the aggressive and difficult-to-treat KRAS cancer gene.
How Baby’s Genes Influence Birth Weight And Later Life Disease
The large-scale study could help to target new ways of preventing and treating these diseases.
Genes Underlying Dogs’ Social Ability Revealed
The social ability of dogs is affected by genes that also seem to influence human behaviour, according to a new study from Linköping University in Sweden.
‘Cellbots’ Chase Down Cancer, Deliver Drugs Directly to Tumors
Programmable T cells shown to be versatile, precise, and powerful in lab studies.
Modified Yeast Shows Plant Response to Key Hormone
Researchers have developed a toolkit based on modified yeast to determine plant responses to auxin.
ReadCoor Launched to Commercialize 3D Sequencing Tech
ReadCoor will leverage the Wyss Institute’s method for simultaneously sequencing and mapping RNAs within cells and tissues to advance development of diagnostics.
NCI Collaborates with Multiple Myeloma Research Foundation
NCI collaborates with MMRF to incorporate genomic and clinical data into NCI Genomic Data Commons database.
Epigenetic Clock Predicts Life Expectancy
New research finds 5 percent of population ages faster, faces shorter lifespan.
Regulatory RNA Essential to DNA Damage Response
Researchers discover a tumour suppressor is stabilized by an RNA molecule, which helps cells respond to DNA damage.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!