Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

NIH Awards Focus on Nanopore Technology For DNA Sequencing

Published: Monday, September 09, 2013
Last Updated: Monday, September 09, 2013
Bookmark and Share
The use of nanopore technology aimed at more accurate and efficient DNA sequencing is the main focus of grants awarded by the NIH.

Nearly $17 million to eight research teams has been awarded through the National Human Genome Research Institute Advanced DNA Sequencing Technology program.  

"Nanopore technology shows great promise, but it is still a new area of science. We have much to learn about how nanopores can work effectively as a DNA sequencing technology, which is why five of the program's eight grants are exploring this approach," said Jeffery A. Schloss, Ph.D., program director for NHGRI's Advanced DNA Sequencing Technology program and director of the Division of Genome Sciences.

Nanopore-based DNA sequencing involves threading single DNA strands through tiny pores. Individual base pairs -- the chemical letters of DNA - are then read one at a time as they pass through the nanopore. The bases are identified by measuring the difference in their effect on current flowing through the pore. For perspective, a human hair is 100,000 nanometers in diameter; a strand of DNA is only 2 nanometers in diameter. Nanopores used in DNA sequencing are 1 to 2 nanometers in diameter.

This technology offers many potential advantages over current DNA sequencing methods, said Dr. Schloss. Such advantages include real-time sequencing of single DNA molecules at low cost and the ability for the same molecule to be reassessed over and over again. Current systems involve isolating DNA and chemically labeling and copying it. DNA has to be broken up, and small segments are sequenced many times. Only the first step of isolating the DNA would be necessary with nanopore technology. 

Innovation is crucial in these as well as the other (non-nanopore) studies being funded. For example, one research team eventually hopes to use light to sequence DNA on a cell phone camera chip for under $100. 

The new grants are awarded to:  

University of Illinois, Urbana-Champaign, $2.47 million over four years (pending available funds) Principal Investigator: Oleksii Aksimentiev, Ph.D.

Dr. Aksimentiev and his colleagues plan to use nanopores as sensors. The researchers are studying the effects of combining synthetic nanopores with a light-based technique to control the flow of DNA molecules through the pores. They will use a type of spectroscopy to read the chemical sequence of the DNA.  

University of New Mexico Health Sciences Center, Albuquerque, $1.35 million over three years (pending available funds) Principal Investigator: Jeremy Edwards, Ph.D.

Dr. Edwards and his colleagues plan to develop innovative molecular biology tools to improve whole-genome sequencing, which entails reading a person's entire genetic blueprint. The researchers hope that better methods of preparing the DNA molecules for sequencing will help scientists identify and link genetic variants to disease and, ultimately, lead to new treatments.  

University of Washington, Seattle, $3.83 million over four years (pending available funds) Principal Investigator: Jens Gundlach, Ph.D.

The researchers plan to continue developing the use of nanopore DNA sequencing technology involving a type of protein nanopore called MspA. Part of their research will focus on improving the control of movement of DNA through the nanopore and on developing algorithms to identify DNA bases.

Columbia University, New York City, $5.25 million over three years (pending available funds) Principal Investigators: Jingyue Ju, Ph.D., George M. Church, Ph.D., (Harvard Medical School, Boston) and James John Russo, Ph.D. (Columbia University, New York City) 

Dr. Ju and his colleagues plan to develop a miniaturized electronic system using nanopores to analyze single molecules of DNA in real time. They will construct large arrays of nanopores to create DNA sequencing chips, enabling them to determine DNA bases during a specific biochemical reaction. They hope this technique will enable them to read large sections of DNA more accurately and rapidly than is now possible. 

Eve Biomedical, Inc., Mountain View, CA., $493,000 over two years (pending available funds) Principal Investigator: Theofilos Kotseroglou, Ph.D. 

Dr. Kotseroglou's research team intends to develop a DNA sequencing system that can sequence an entire human genome for under $100. The overall system will be based on using light to sequence DNA on a cell phone camera chip. For now, his group plans to continue studying ways to accurately read long sections of DNA and develop software tools and bioinformatics.

University of Massachusetts, Amherst, $1.07 million over four years (pending available funds) Principal Investigator: Murugappan Muthukumar, Ph.D.

Dr. Muthukumar's research group plans a theoretical approach to study several major challenges underlying nanopore-based DNA sequencing, including slowing down the rate at which DNA molecules flow through the pores, the effects of specific ions, changes in the shape of the DNA molecule and other aspects of the environment.

University of North Carolina at Chapel Hill, $2.05 million over four years (pending available funds) Principal Investigator: John Michael Ramsey, Ph.D.

Dr. Ramsey and his co-workers plan to develop a low-cost method for rapidly mapping individual genomes. Such maps will help determine how large mutations in DNA structure contribute to human disease and improve diagnostic testing using genomics. 

Electronic Biosciences, Inc., San Diego, $239,000 Principal Investigator: Anna Schibel, Ph.D.

Dr. Schibel and her co-workers will develop chemical methods to slow the rate by which single-stranded DNA molecules pass through protein nanopores. Such approaches may enable the development of faster, lower-cost DNA sequencing techniques.    

The costs of DNA sequencing have greatly declined since 2003, when the genome sequencing performed under the Human Genome Project was completed at a cost of approximately $1 billion. Only a year later, in 2004, sequencing a human genome cost an estimated $10-50 million, thanks to improvements in technologies and tools. By 2009, NHGRI met its goal of producing high-quality human genome sequences at a 100-fold reduction in price, or $100,000. While achieving another 100-fold drop in price has been difficult, sequencing a person's genome today costs about $5,000 to $6,000 <http://www.genome.gov/sequencingcosts>.

The grant numbers of the awards are the following: R01 HG007406; R01 HG006876; R01 HG005115; R01 HG007415; R43 HG007386; R01 HG002776; R01 HG007407; and R43 HG006878.

Additional information about NHGRI can be found below.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Study Shows Promise of Precision Medicine for Most Common Type of Lymphoma
The study appeared online July 20, 2015, in Nature Medicine.
Tuesday, July 21, 2015
NIH Study Identifies Gene Variant Linked to Compulsive Drinking
Mice carrying the Met68BDNF gene variant would consume excessive amounts of alcohol.
Tuesday, July 21, 2015
In Blinding Eye Disease, Trash-Collecting Cells Go Awry, Accelerate Damage
NIH research points to microglia as potential therapeutic target in retinitis pigmentosa.
Friday, July 03, 2015
Potential Therapeutic for Blinding Eye Disease
NIH research points to microglia as potential therapeutic target in retinitis pigmentosa.
Thursday, July 02, 2015
NCI-MATCH Trial will Link Targeted Cancer Drugs to Gene Abnormalities
Precision medicine trial will open to patient enrollment in July.
Tuesday, June 09, 2015
A New Role for Zebrafish: Larger Scale Gene Function Studies
A relatively new method of targeting specific DNA sequences in zebrafish could dramatically accelerate the discovery of gene function and the identification of disease genes in humans.
Monday, June 08, 2015
NIH Researchers Pilot Predictive Medicine by Studying Healthy People’s DNA
New study sequence the genomes of healthy participants to find “putative,” or presumed, mutations.
Friday, June 05, 2015
Linking Targeted Cancer Drugs to Gene Abnormalities
Investigators at the NIH have announced a series of clinical trials that will study drugs or drug combinations that target specific genetic mutations.
Wednesday, June 03, 2015
Scientists Create Mice with a Major Genetic Cause of ALS and FTD
NIH-funded study provides new platform for testing treatments for several neurodegenerative disorders.
Friday, May 22, 2015
Mice With a Major Genetic Cause of ALS and FTD Created
NIH-funded study provides new platform for testing treatments for several neurodegenerative disorders.
Thursday, May 21, 2015
New Insights into How DNA Differences Influence Gene Activity, Disease Susceptibility
NIH-funded pilot study provides a new resource about variants across the human genome.
Friday, May 08, 2015
Souped-up Remote Control Switches Behaviors On-and-Off in Mice
BRAIN Initiative yields chemical-genetic tool with push-pull capabilities.
Thursday, May 07, 2015
NIH-funded Study Points Way Forward for Retinal Disease Gene Therapy
Benefits for Leber congenital amaurosis peak after one to three years, then diminish.
Tuesday, May 05, 2015
Possible Treatment for Lethal Pediatric Brain Cancer
NIH-funded preclinical study suggests epigenetic drugs may be used to treat leading cause of pediatric brain cancer death.
Tuesday, May 05, 2015
Statement on NIH Funding of Research Using Gene-Editing Technologies in Human Embryos
Researchers modify the gene responsible for a potentially fatal blood disorder using CRISPR/Cas9 technology.
Saturday, May 02, 2015
Scientific News
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
Understanding the Molecular Origin of Epigenetic Markers
Researchers at IRB Barcelona discover the molecular mechanism that determines how epigenetic markers influence gene expression.
New Tech Enables Epigenomic Analysis with a Mere 100 Cells
A new technology that will dramatically enhance investigations of epigenomes, the machinery that turns on and off genes and a very prominent field of study in diseases such as stem cell differentiation, inflammation and cancer has been developed by researchers at Virginia Tech.
Access Denied: Leukemia Thwarted by Cutting Off Link to Environmental Support
A new study reveals a protein’s critical – and previously unknown -- role in the development and progression of acute myeloid leukemia (AML), a fast-growing and extremely difficult-to-treat blood cancer.
New Weapon in the Fight Against Blood Cancer
This strategy, which uses patients’ own immune cells, genetically engineered to target tumors, has shown significant success against multiple myeloma, a cancer of the plasma cells that is largely incurable.
Toxin from Salmonid Fish has Potential to Treat Cancer
Researchers from the University of Freiburg decode molecular mechanism of fish pathogen.
Study Finds Non-Genetic Cancer Mechanism
Cancer can be caused solely by protein imbalances within cells, a study of ovarian cancer has found.
Scientists Create CRISPR/Cas9 Knock-In Mutations in Human T Cells
In a project spearheaded by investigators at UC San Francisco, scientists have devised a new strategy to precisely modify human T cells using the genome-editing system known as CRISPR/Cas9.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!