Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

IDT Reduces Undesirable Effects of RNAi Protocols

Published: Wednesday, March 12, 2014
Last Updated: Wednesday, March 12, 2014
Bookmark and Share
Learn about the latest advances at the Aptamers and RNAi conferences in Oxford, UK.

Integrated DNA Technologies (IDT) will present two keynote addresses at the upcoming RNAi International Conference and Exhibition and its co-hosted symposium, Aptamers.

Providing a forum for the discussion of long and short non-coding RNAs, their role in gene regulation, functional studies, RNA biomarkers and therapeutic application, IDT CSO Dr Mark Behlke will share IDT’s expertise within the advancing field of RNAi.

Forming part of the Aptamers track (24-25 March), Dr Behlke will discuss how aptamers can be employed as a targeting ligand to deliver therapeutic cargo to specific cell types. Such cargo can be attached following aptamer synthesis using reactive chemistries or through hybridization to a ‘stick’ domain.

Attachment using the ‘stick’ domain is highly flexible and allows the use of a broad variety of cargo types. However the addition of the ‘anti-stick’ sequence to facilitate release of the cargo can prove problematic, with reduced siRNA potency often experienced. Design considerations to minimize these undesirable effects will therefore be explored.

Off-target effects are a chronic problem for studies employing RNAi, since the seed region directs suppression of unknown targets via miRNA. Bioinformatic methods have been developed, however a 7mer seed region has thousands of possible binding sites and it is extremely difficult to predict which of these are significant. Chemical methods can reduce the incidence of these effects, but will cause a reduction in the potency of the siRNA.

During RNAi (25-27 March), Dr Behlke will discuss Unlocked Nucleic Acids (UNAs), which have previously been proven useful in the reduction of off-target effects while retaining potency.

This presentation will cover the use of UNAs in Dicer-substrate siRNAs (DisRNA), comparing position-specific UNA effects on seed region off-target effects and potency.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

TBUSA, IDT Announce Collaboration
Collaboration aims to support targeted RNA Sequencing.
Wednesday, April 27, 2016
IDT Acquires AITbiotech
Acquisition increases capacity to deliver high quality, locally manufactured oligos .
Thursday, December 10, 2015
IDT Expands Oligonucleotide Manufacturing in Singapore
Acquisition increases capacity to deliver high quality, locally manufactured oligos.
Wednesday, December 02, 2015
Dr Patricia Tavormina Named Winner of First IDT ISO 14001 Sustainability Award
Biodiversity research on methane sinks and global warming earns $14,001 in oligo credit.
Thursday, September 10, 2015
Untangling the Web of Gene Expression
New technology for understanding gene expression drives research forward.
Wednesday, May 20, 2015
Discover Affordable Custom NGS Panels
IDT webinar helps NGS researchers bring cost-savings to target capture panels.
Thursday, February 12, 2015
Synthetic Biology Webinar - Genome Modifications Made Easy
IDT reveals how to overcome the challenges of CRISPR-mediated genome editing.
Thursday, January 15, 2015
Discover the Latest NGS-Based Assays Guiding Precision Oncology
IDT joins forces with Foundation Medicine in AMP workshop.
Saturday, November 08, 2014
IDT Acquires SURVEYOR® Nuclease Business from Transgenomic
Addition of SURVEYOR enzyme and kits expands IDT's molecular biology product offerings.
Wednesday, July 02, 2014
World Cup 2014 - with a Scientific Twist
IDT fuses fruit flies, football and fun in a unique global contest with great prizes.
Thursday, June 12, 2014
Summit Partners Makes Growth Equity Investment in IDT
IDT’s founder, Dr. Joseph Walder, will remain the company’s majority owner.
Wednesday, March 19, 2014
Enhanced Molecular Inversion Probes for NGS Target Enrichment
IDT presents latest developments at 15th annual Advances in Genome Biology and Technology meeting.
Tuesday, February 04, 2014
Providing a Rapid and Reliable Method of Gene Cloning
Integrated DNA Technologies will be speaking at the upcoming Antibody Engineering and Therapeutics conference.
Monday, December 02, 2013
IDT Provides Insights into the World of Gene Construction
Informative and educational talk to take place at SynBioBeta 2013.
Thursday, November 14, 2013
Discussing the Science of Big DNA
Read about the most recent advances in synthetic biology in the latest issue of DECODED.
Monday, September 23, 2013
Scientific News
Liquid Biopsies: Miracle Diagnostic or Next New Fad?
Thanks to the development of highly specific gene-amplification and sequencing technologies liquid biopsies access more biomarkers relevant to more cancers than ever before.
Discovered Through ‘Big Data’ Analysis
Researchers at the SBP have identified over 100 new genetic regions that affect the immune response to cancer.
New Therapeutic Targets For Small Cell Lung Cancer Identified
Researchers at UTSW Medical Center have identified a protein termed ASCL1 that is essential to the development of small cell lung cancer and that, when deleted in the lungs of mice, prevents the cancer from forming.
Deciphering Inactive X Chromosomes
Untangling the Barr body of inactive X chromosomes valuable for understanding chromosome structure and gene expression.
Micro Disease-Detecting Senor Created
Researchers at McMaster University have created a microscopic disease-detecting sensor that can turn on to detect trace amounts of substances.
Liquid Biopsies Treating Ovarian Cancer
Researchers have discovered a promising monitor and treat recurrence of ovarian cancer. Detecting cancer long before tumours reappear.
Uncovering a New Principle in Chemotherapy Resistance in Breast Cancer
The NIH study has revealed an entirely unexpected process for acquiring drug resistance that bypasses the need to re-establish DNA damage repair in breast cancers that have mutant BRCA1 or BRCA2 genes.
Understanding Treatment Resistant Melanoma
Researchers have determined how advanced melanoma becomes resistant; a development toward developing treatments.
Investigating ‘Black Box’ of Human Genetics
Investigations into inactive X chromosomes have shown unusual DNA repeat elements are essential for maintaining 3D structure.
Liquid Biopsies: DNA Size Matters
Study finds circulating tumour DNA can be distinguished from healthy DNA through fragment size identification.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!