Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Powerful Tool for Genomic Editing

Published: Friday, May 23, 2014
Last Updated: Friday, May 23, 2014
Bookmark and Share
CRISPR/Cas9 - Designed to produce a genetically modified cell.

AMSBIO has announced the introduction of CRISPR/Cas9 - a full gene editing tool designed to produce a genetically modified cell using any mammalian cell line and targeting any gene.

CRISPR/Cas9 is revolutionizing the field of genomic editing by providing scientists with a powerful tool able to change any gene, in any cell in a highly targeted manner and without introducing foreign DNA.

The benefits of CRISPR/Cas9 over previous forms of gene editing, such as TALENs and zinc finger, are that it is much simpler to implement and has higher efficiency at performing bi-allelic gene modifications.

Using CRISPPR/Cas9 technology it is now possible to create knock-in, knock-outs and mutations of any gene in any cell line.

The latest tool in genome editing - CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The CRISPR/Cas9 genome editing system requires the co- expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter.

With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3' end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.

The functional cassette synthesized in the rescue donor vector can then be inserted into the unwound DNA. The repaired genome will now express users desired sequence with or without tags.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Ready-to-Use Tagged cDNA Clones
Available from AMSBIO TrueORF®cDNA clones are tagged cDNA clones for protein studies.
Monday, June 01, 2015
Webinar Discusses DNA Damage in Individual Cells
New on-demand webinar from AMSBIO.
Monday, September 30, 2013
Informative Guide to Gene Silencing & Delivery
AMSBIO’s new guide - 'Top Ten Tips for Gene Silencing & Delivery'.
Tuesday, June 19, 2012
Knockdown Cell Lines for DNA Repair Studies
New range of 20 DNA Repair Knockdown cell lines enables scientists to study the molecular etiology of tumour genomic instability and to exploit it in oncology research.
Wednesday, February 23, 2011
Scientific News
Long Telomeres Associated with Increased Lung Cancer Risk
Genetic predisposition for long telomeres predicts increased lung adenocarcinoma risk.
Expanding the Brain
A team of researchers has identified more than 40 new “imprinted” genes, in which either the maternal or paternal copy of a gene is expressed while the other is silenced.
Identifying a Key Growth Factor in Cell Proliferation
Researchers discover that aspartate is a limiter of cell proliferation.
Study Uncovers Target for Preventing Huntington’s Disease
Scientists from Cardiff University believe that a treatment to prevent or delay the symptoms of Huntington’s disease could now be much closer, following a major breakthrough.
The Genetic Roots of Adolescent Scoliosis
Scientists at the RIKEN Center for Integrative Medical Sciences in collaboration with Keio University in Japan have discovered a gene that is linked to susceptibility of Scoliosis.
A Gene-Sequence Swap Using CRISPR to Cure Haemophilia
For the first time chromosomal defects responsible for hemophilia have been corrected in patient-specific iPSCs using CRISPR-Cas9 nucleases
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
Understanding the Molecular Origin of Epigenetic Markers
Researchers at IRB Barcelona discover the molecular mechanism that determines how epigenetic markers influence gene expression.
New Tech Enables Epigenomic Analysis with a Mere 100 Cells
A new technology that will dramatically enhance investigations of epigenomes, the machinery that turns on and off genes and a very prominent field of study in diseases such as stem cell differentiation, inflammation and cancer has been developed by researchers at Virginia Tech.
Access Denied: Leukemia Thwarted by Cutting Off Link to Environmental Support
A new study reveals a protein’s critical – and previously unknown -- role in the development and progression of acute myeloid leukemia (AML), a fast-growing and extremely difficult-to-treat blood cancer.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!