Bioo Scientific’s NEXTflex™ 16S V1 – V3 Amplicon-Seq Kit simplifies bacterial metagenomics studies for sequencing on the Illumina® MiSeq platform. This kit allows users to go from sample to sequence in two hours, making it the fastest library prep kit available. Up to 384 barcodes are available with this kit, offering extreme flexibility and multiplexing power to rRNA sequencing. Sequencing primers are included in the kit eliminating the need for custom synthesis.
16S V1-V3 amplicon processing involves two main steps: an initial PCR amplification using customized PCR primers that target the V1-V3 domains, and a subsequent PCR amplification that integrates relevant flow cell binding domains and unique 12 base pair sample indices. The number of cleanup steps is limited to ensure maximum recovery of amplicons for downstream sequencing.
The NEXTflex 16S V1 – V3 Amplicon-Seq Kit simplifies bacterial diversity studies, studies of interactions between host species and bacterial communities, identification of non-culturable bacteria, detection of adventitious agents and enzyme discovery and production.
The NEXTflex 16S V1 – V3 Amplicon-Seq Kit is the latest addition to Bioo Scientific’s line of library prep kits for Illumina-compatible metagenomics analysis, which includes the NEXTflex™ 16S V4 Amplicon-Seq Kits.