Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Lucigen, Thermo Fisher to Release New Mate Pair Library Technology

Published: Wednesday, August 27, 2014
Last Updated: Wednesday, August 27, 2014
Bookmark and Share
The Ion PGM is a fast and cost-effective platform for next generation sequencing applications.

The release of the Ion TrueMate™ Library Kit will expand the platform’s capabilities by allowing long mate pair libraries up to 8 kb to be run on the PGM. Mate pair libraries are critical for de novo genome assembly, chromosomal rearrangement studies, and finishing genomes. Traditional fragment libraries produce large quantities of data but result in sequence gaps, misassembled contigs, and collapsed repeats due to short read lengths.  Combining mate pair libraries with fragment libraries has been proven to achieve more accurate genome assemblies.

Developed at Lucigen using their NxSeq® mate pair technology, the Ion TrueMate Library Kit includes multiple innovations that enable high fidelity mate pair libraries without paired end sequencing on the PGM platform.  The protocol and reagents have been optimized to eliminate chimera formation, with the inclusion of patent-pending ChimeraCode™ sequences allowing confirmation of actual mate pairs via bioinformatics.  The TrueMate libraries demonstrate >90% efficiency of real mate pairs, compared to 30% or less with other platforms.  Lucigen’s new Accura™ high fidelity polymerase is also incorporated into the new product.

“Existing mate pair libraries contain a high percentage of chimeras that result in misassembled genomes. The mate pairs that are obtained from the TrueMate libraries achieve >90% efficiency, and when combined with Ion Fragment libraries, have shown tremendous promise for closing genomes, finishing BAC sequences, and obtaining accurate scaffold assemblies,” states Lucigen CEO and Founder Dr. David Mead.

The TrueMate Library Kit will be sold for the PGM platform exclusively through Thermo Fisher, while Lucigen will perform library creation and sequencing services for customers globally.

The release of the Ion TrueMate library kit is the first of multiple mate pair kits in development by Lucigen.  Future products will include the ability to create longer mate pairs of 20 kb or higher and the application of whole transcriptome sequencing via Full Length RNASeq protocols.

For research use only.  Not for use in diagnostic procedures.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,600+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
Fix for 3-Billion-Year-Old Genetic Error
Researchers at The University of Texas at Austin have developed a fix that allows RNA to accurately proofread for the first time.
Higher Frequency of Huntington's Disease Mutations Discovered
University of Aberdeen study shows that the gene change that causes Huntington's disease is much more common than previously thought.
Revealing the Genetic Causes of Bowel Cancer
A landmark study has given the most detailed picture yet of the genetics of bowel cancer — the UK's fourth most common cancer.
The Epigenetic Influences of Chronic Pain
Researchers at Drexel University College of Medicine are aiming to identify new molecular mechanisms involved in pain.
Fighting Resistant Blood Cancer Cells
Biologists present new findings on chronic myeloid leukemia and possible therapeutic approaches.
Tumor Cells Develop Predictable Characteristics
Scientists have discovered that cancer cells at the edge of a tumor that are close to the surrounding environment are predictably different from the cells within the interior of the tumor.
Mothers Obesity Could be Passed on in mtDNA
Obesity can predispose offspring in multiple generations to metabolic problems.
New Imaging Method Reveals Nanoscale Details about DNA
Enhancement to super-resolution microscopy shows orientation of individual molecules, providing a new window into DNA’s structure and dynamics.
Genetic Research Can Significantly Improve Drug Development
With drug development costs topping $1.2bn (£850 million) to get a single treatment to the point it can be sold and used in the clinic, could genetic analysis save hundreds of millions of dollars?
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!