Sigma-Aldrich has announced that it is working together with the premier scientific journal Cell to offer the article on High Content Viral Library Screens to a wider audience.
Written by principal members of The RNAi Consortium (TRC) and published in the March 24, 2006, issue of Cell, the full-text article is now available through the Sigma-Aldrich corporate Web site.
The authors J. Moffat et al. reported the creation of a lentiviral shRNA library to knock down genes for genome-wide screening.
In addition, the authors demonstrated the efficacy of the technology and, specifically, the TRC lentiviral shRNA library for high content screening.
"Our goal is to offer researchers innovative technologies, products and information in the latest cutting-edge fields of discovery," said Stephanie Uder, Functional Genomics Product Manager at Sigma-Aldrich.
"Enabling public access to this and other full-text articles on the latest RNAi technologies is but another example of our commitment to helping our customers to accelerate, innovate and create."
In the Cell publication, the scientists reported development of the TRC lentiviral-based shRNA library that could be used for loss-of-function screens in a wide range of mammalian cell types.
Such cell types include primary cells, non-dividing cells, cell lines not amenable to transfection, as well as standard transfectable lines.
Moffat and colleagues employed a screen based on high content imaging to identify genes required for mitotic progression in human cancer cells. The screen utilized 5,000 individual clones targeting 1,028 human genes.
Several issues related to the use of RNAi as a screening method were addressed in the article, including efficient gene transfer, specificity and efficiency of knockdown, and other technical requirements.
The TRC shRNA library currently consists of 104,000 clones targeting over 22,000 human and mouse genes, most of which are already available from Sigma-Aldrich (a member and distributor of TRC) as the MISSION™ TRC collections.
To download the Cell article "A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High Content Screen," written by Moffat et al., visit Sigma-Aldrich website.