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THe AtSCL26 transcription factor controls cross-talk between GA and N root architecture in Arabidopsis thaliana roots
Beatriz Lagunas, Anthony D. Carter, Dafyd Jenkins and Miriam Gifford

Phenotypic and molecular evidence supports the hypothesis that developmental program enabling nodule formation arose during evolution from a lateral root ‘blueprint’ pre-existing in all higher plants . We reasoned that analyzing Arabidopsis genes orthologous to regulators of nodulation could shed insight on control of lateral root development. This led us to the discovery that an Arabidopsis GRAS family transcription factor controls lateral root development under specific nitrogen conditions.

LOHA Comprehensive Assay for Single Nucleotide Polymorphism, Copy Number Variants and Loss of Heterozygosity Using SureSelect Target Enrichment
Kyeong Soo Jeong, Arjun Vadapalli, Ashutosh Ashutosh, Paula Costa, Brian Peter, Stephanie Fulmer-Smentek, Magnus Isaksson, Jayati Ghosh, Douglas Roberts, Holly Hogrefe

Here we describe a comprehensive assay that enables researchers to identify SNP, INDEL, CNV, and LOH using SureSelect target enrichment. This design can be employed as a standalone entity or in concert with other bait designs for SNP and INDEL detection. We also describe methods for data analysis and visualization.

An examination of specific cellular organelle-targeting nanotags using combined 3D Raman and SERS imaging
Katherine Lau, Sarah McAughtrie, Karen Faulds, Duncan Graham

We investigated the specific targeting of endoplasmic reticulum and trans-Golgi network in Chinese hamster ovarian cells using functionalised nanotags. The targeting was examined using the combined 3D SERS and Raman imaging method.

The Power Decoder simulator for the evaluation of pooled shRNA screen performance
Jesse Stombaugh, Abel Licon, Žaklina Strezoska, Joshua Stahl, Sarah Bael Anderson, Michael Banos, Anja van Brabant Smith, Amanda Birmingham, Annaleen Vermeulen

Power Decoder (written in R and Python) simulates shRNA pooled screening experiments in silico to allow for the estimation of a screen’s statistical power. Populations of shRNAs were engineered in such a way that the magnitude of depletion and enrichment was known, then using the negative binomial distribution, an in silico model was developed to successfully resemble data from an actual laboratory experiment.

Polymer Microarrays for Biomaterial Development
Simmonte, M.J.1, Dhaliwal, K.2, Cuschieri, K.3, Graham, S.V.4, Bradley, M.1

The application of polymer microarrays in the discovery of biocompatible and bioactive substrates. Progress towards biomaterial development for the treatment of SIRS (systemic inflammatory response syndrome), and improving cervical cytology.

Knockdown of long noncoding RNAs in breast cancer
1 Jennii Luu, 2 Jesper Maag, 1 Yanny Handoko, 3 Richard Redvers, 3,4 Robin L. Anderson, 5 Maren M. Gross , 2 Marcel E. Dinger, and 1,3 Kaylene J. Simpson 1 Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre; 2 Genome Informatics, The Kinghorn Cancer Centre, The Garvan Institute of Medical Research; 3 Metastasis Research Laboratory, Peter MacCallum Cancer Centre, 4 Sir Peter MacCallum Department of Oncology, University of Melbourne;

RNAi global collaboration study using Lincode siRNA in a primary screen of tumor and nontumor breast cell lines. Hundreds of lncRNAs are found to affect viability and cell morphology of breast cancer. Presented at Keystone Symposia on Long Noncoding RNAs: From Evolution to Function, Mar 15 - Mar 20, 2015.

DETERMINATION OF THE QUALITY OF ACTIVE INGREDIENTS IN PAIN KILLERS USING GC-MS
Elizabeth N.M Murago1, Nathan Oyaro1, Anthony N. Gachanja, Onditi O. Anam, Felix M. Mawili, Steve Lancaster

From this study, the pain killers sampled were found to have large error bars suggesting that there exist counterfeit drugs in the market. The brands mostly affected for analysis of acetaminophen were panadol, action, P500, P5500, elymol and neladol. The error bars for caffeine analysis were quite low indicating that all tablets either counterfeit or original maintained the same amount of this active ingredient.

DETERMINATION OF ESSENTIAL AND HEAVY METALS IN KENYAN HONEY BY ATOMIC ABSORPTION AND EMMISSION SPECTROSCOPY
A. Mbiri, A. Onditi, N. Oyaro

Generally, honey samples recorded very low concentrations of As compared to the other heavy metals determined in this study. The highest concentration of As was recorded in honey sample from Nairobi with a value of 0.03±0.01 ppm. All the values recorded were within the KEBS permitted value of 0.5 ppm in food according to Kenya Bureaue of Standards, 1996.

Prospecting For Iron and Titanium Using Termitaria
Kinachu James. G, Kiptoo Jackson. K, Onditi Anam.O.

This study demonstrated elevated levels of the two metals (that is iron and titanium) in termitaria soil compared to the surrounding soils. Termitaria are very important bioturbators and can therefore be used as preliminary step in mineral prospecting since they provide an indication of the potential of the positive anomaly, and enables a judgment on the scale of the metal accumulation

Human iPSC-derived hepatocytes and cardiomyocytes for drug toxicity testing
AnnandRR; Vardaro R; Hamilton B; Akakira R; Tamura K; Yoshida S; Lin YC; Toyoda D; Kogami H; Okuda Y; Watanabe T; Inamura M

Human iPS-derived hepatocytes (ReproHepato™) and cardiomyocytes (ReproCardio 2™) are useful for in vitro toxicity assays.

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Showing Results 1 - 10 of 310
Scientific News
Results from the Sidney Kimmel Cancer Center at Thomas Jefferson University
The Sidney Kimmel Cancer Center has published results of its work evaluating the potential for ANGLE’s Parsortix system.
Unprecedented Microbial Diversity in Isolated Amazonian Tribe
Scientists from the Icahn School of Medicine have discovered the most diverse collection of bacteria yet in humans among an isolated tribe of Yanomami Amerindians in the remote Amazonian jungles of Venezuela.
New Genetic Mutation Could Signal Start Of Malaria Drug Resistance In Africa
Early indicators of the malaria parasite in Africa developing resistance to the most effective drug available have been confirmed, according to new research published in Antimicrobial Agents and Chemotherapy.
Gene Variant Linked to Smoking Longer, Getting Lung Cancer Sooner
Smokers with a particular variation in a nicotine receptor gene were more likely to continue smoking for four years.
Scientists Discover Protein That Boosts Immunity to Viruses and Cancer
Researchers now developing a gene therapy designed to boost the infection-fighting cells.
Paternal Sperm May Hold Clues to Autism
Tags on DNA from fathers’ sperm linked to children’s autism symptoms.
NIH Study Finds Genetic Link for Rare Intestinal Cancer
Researchers recommend screening for people with family history.
Potential For Prediction Of Progression For Early Form Of Breast Cancer
Scientists in Manchester have identified a way to potentially predict which patients with an early form of breast cancer will experience disease progression.
Systems-Wide Genetic Investigation Of Blood Pressure Regulation
A genetic investigation of individuals in the Framingham Heart Study may prove useful to identify novel targets for the prevention or treatment of high blood pressure.
Tumor-Only Genetic Sequencing May Misguide Cancer Treatment in Nearly Half of All Patients
Johns Hopkins scientists say the genetic code of tumors must be compared to patients’ noncancer genome to get a true picture.
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