|Combination of a transient assay based ChIP technology and transcriptome analyses for the exploration of potential transcription factor binding sites|
Shu-Jen Chou (presenter), Li-Teh Chen, Yi-Hang Li, Chiung-swey Joanne Chang and Shu-Hsing Wu
1. Combination of protoplast transient expression system and Nickel resin based purification is an useful approach for chromatin enrichment that can be used to identify transcription factor’s potential target genes. 2.Comparison our ChIP-on-chip target genes with in vivo binding targets using two whole genome tiling array platforms showing high degree of overlap between two methods.
|A high-throughput protein array-based approach |
Rachel Van Dyk, Claudia Kirisits, Paul Potter, Fook Tim Chew, Reinhard Hiller
Here we present a novel discovery-oriented high-throughput approach to the screening of allergen reactivates in crude biological extracts is presented using seafood allergens as an example. The CPGR workflow permits the effective screening of hundreds of putative allergens in parallel using minute amounts of patient serum and constitutes a cost-efficient allergen-specific antibody screening method for a routine diagnostic setting.
|Apoptosis Inducing Novel microRNA for Breast cancer and Hepatocellular Carcinoma|
Arutselvan Natarajan, Sally J. DeNardo, Mark A. Zern, Senthil K. Venugopal
MicroRNA provides a mechanism to target cancer stem cells and restraint tumors cells permanently; this could lead to promising cancer therapeutics and imaging agents. Apoptosis inducing microRNA was identified to target HCC and breast cancer cells for imaging and therapy.
|SOME BIOLOGICAL PROPERTIES OF TRITERPENOID ACIDS|
S.N. Morozkina, O.I. Antimonova, E.M. Eremenko*, A.G. Shavva
Ursolic and betulinic acids acids induce the processes of apoptosis. We tested the risk of osteoporosis induction under influence of triterpenoids, since they can interact with glucocorticoid receptors. In experiments on oviriectomized rats we found that acids do not show any negative influence on percentage of mineral components in the femur and have cholesterol-lowering action under experimental conditions.
|THE OSTEOPROTECTIVE ACTION OF 6-OXA-8a-ANALOGUES OF STEROID ESTROGENS|
A.G. Shavva, V.N. Belov, A.Yu. Solovyev, S.N. Morozkina
We synthesized fourteen 6-oxa-8a-estrogens analogues and investigated osteoprotective and uterotropic actions. We demonstrated correlation: every modification in structure of 6-oxa-8-analogues leading to strong (>30%) reduction of uterotropic action induces slump of osteoprotective activity. This allows to make conclusion: main biotarget, responsible for appearance of osteoprotective action is a-estrogen receptor. We found steroid estrogen analogues with cholesterol-lowering properties without u
|A Novel Fluorimetric Assay for the Detection of TACE (a-Secretase)|
Vera Rakhmanova, Xudong Zhu, Fengying Li, Xiaofen Zhong, Anita Hong and Xiaohe Tong
To facilitate high throughput screening of TACE inhibitors, AnaSpec synthesized a novel peptide substrate for TACE using QXL™ 520/5-FAM FRET pair. Using this FRET substrate, AnaSpec developed a new kit – the SensoLyte™ 520 TACE Activity Assay. This kit can be used to detect the activity of the enzyme and for screening of TACE inhibitors. It is highly sensitive and can detect subnanogram amounts of enzyme.
|High Content Image Analysis using a Laser Scanning Microplate Cytometer|
Paul Wylie, Ben Schenker, Sarah Payne and Andrew Goulter
Microscope-based high-content instruments offer high optical resolution, however, the limited field of view afforded by their objective lenses can mean lengthy read times for some assays, especially where multiple image capture per well is required. Wherever possible, only a small percentage of the total number of cells present in a test well are analysed to keep plate read times at a minimum, which may not always be ideal. Microplate laser- scanning cytometers, such as TTP LabTech’s Acumen® eX3
|A genome-wide loss of function screen identifies new genes required for lung cancer cell proliferation|
PINNA G, ARAUJO N, MOROZOVA N, DETREZ MA & HAREL-BELLAN A
RNA interference (RNAi) provides an experimental tool for functional genomics analysis. We screened a genome-wide RNAi library to identify new genes involved in lung cancer cell proliferation. 72 % from the 257 genes identified were involved in general gene expression processes, 16 % were of unknown function or unrelated to proliferation, and 12% consisted of uncharacterized genes. These last sets of genes provide potential novel targets for lung cancer treatment.
|Specific Ligand-Based Depletion of Human Albumin and IgG in the Presence of Denaturants|
Todd Sanderson, Hongshan Li, Brian Miller, Laura Alionte, and Lisa Bradbury
Poster data highlights the advantages of a ligand-based depletion method that functions in the presence of urea and is highly specific, disposable, cost effective, and flexible.