|Efficient downregulation of the lung liquid clearing gammaENaC subunit by RNAi|
Nihal Yueksekdag, Marei Drechsel, Christa Schmidt, and Josef Rosenecker
CF is caused by mutations in the gene encoding for CFTR. CFTR functions as chloride channel on the apical membrane of epithelia thereby regulating the transport of chloride and also sodium ions indirectly. It seems that the regulation of ENaC fails due to the mutated CFTR protein. And it is assumed that ENaC plays a role in the pathogenesis of chronic lung disease in CF-patients.
|Bayesian Parameter Estimation for Systems Biological Models of Dynamic Cellular Networks|
Jarad Niemi, Lingchong You and Mike West
Dynamic cellular networks determine production of proteins in the cells in our body. The Rb-E2F network is a key example: it controls cell cycle, proliferation and degradation - a key network in almost all cancers. We develop statistical models of such single-cell processes - fine-time scale dynamic network models. Model estimation requires informative priors and custom Metropolis proposals.
|Critical factors for successful RNAi experiments in primary cells and hard to transfect cell lines|
Markus Zumbansen1, Nicole Spottke1, Sheila Offizier1, Allison St. Amand2, Devin Leake2, Ludger Altrogge1, Meike Weigel1, Sandra Domzalski1, Dietmar Lenz, and Herbert Müller-Hartmann1
The amaxa Nucleofector® Technology is a well established method for effective, non-viral transfection of any nucleic acid substrate into hard-to-transfect cells, especially suspension and primary cells. With the
Nucleofector® 96-well Shuttle® system high throughput applications such as siRNA-library screenings have become amenable for the first time in these cell types. This renders target validation and identification possible
in cell types highly relevant for biomedical research. Here we dis
|Improving rural livelihoods through development of vegetable-based postharvest technologies in Cambodia, Lao PDR and Vietnam|
Christian Genova, Katinka Weinberger, Jun Acedo, Peter Hanson, Paul Gniffke and Manuel Palada
RETA 6208 is an ADB-funded project whose main goal is to reduce poverty, enhance rural economic development, and improve food security in Cambodia, Lao PDR, and Viet Nam through promotion of the vegetable postharvest sector. The specific objectives are to: (i) reduce postharvest losses of high volume, high value vegetable commodities; and (ii) develop and promote low-cost postharvest technologies in collaboration with both public and private sectors.
|DOCKING STUDIES OF A NEW HETEROCYCLIC METHYLTHIOMORPHOLIN PHENOLS DERIVATIVES AS ANTIHYPERTENSIVE DRUGS WITH ACE TARGET.CASE FESCDIPINE II |
1V. Vázquez,1O. Vázquez, 1,2M.A.Balboa, 1A. Ma. Velázquez, 1V.H. Abrego, 1B.Camacho, 1L. Martínez, 1L.A. Torres, 1A. Ramírez, 1I.Martínez, 3R.López-Castañares, 1S.Díaz-Barriga, 4J. Bocanegra, 4Froylan Gómez-Laguna, 1F. Olvera, 1E.Angeles.
As part of the Drug Design in Medicinal Chemistry Program of the UNAM, new methylthiomorpholinphenol compounds with cardiovascular effect, were studied. One of them, FESCDIPINE II, was an excellent antihypertensive drug, that has low toxicity and preliminary studies indicate that the Angiotensin-converting enzyme (ACE) system is the biological target of this compound.
|Combination of a transient assay based ChIP technology and transcriptome analyses for the exploration of potential transcription factor binding sites|
Shu-Jen Chou (presenter), Li-Teh Chen, Yi-Hang Li, Chiung-swey Joanne Chang and Shu-Hsing Wu
1. Combination of protoplast transient expression system and Nickel resin based purification is an useful approach for chromatin enrichment that can be used to identify transcription factor’s potential target genes. 2.Comparison our ChIP-on-chip target genes with in vivo binding targets using two whole genome tiling array platforms showing high degree of overlap between two methods.
|A high-throughput protein array-based approach |
Rachel Van Dyk, Claudia Kirisits, Paul Potter, Fook Tim Chew, Reinhard Hiller
Here we present a novel discovery-oriented high-throughput approach to the screening of allergen reactivates in crude biological extracts is presented using seafood allergens as an example. The CPGR workflow permits the effective screening of hundreds of putative allergens in parallel using minute amounts of patient serum and constitutes a cost-efficient allergen-specific antibody screening method for a routine diagnostic setting.
|Apoptosis Inducing Novel microRNA for Breast cancer and Hepatocellular Carcinoma|
Arutselvan Natarajan, Sally J. DeNardo, Mark A. Zern, Senthil K. Venugopal
MicroRNA provides a mechanism to target cancer stem cells and restraint tumors cells permanently; this could lead to promising cancer therapeutics and imaging agents. Apoptosis inducing microRNA was identified to target HCC and breast cancer cells for imaging and therapy.
|SOME BIOLOGICAL PROPERTIES OF TRITERPENOID ACIDS|
S.N. Morozkina, O.I. Antimonova, E.M. Eremenko*, A.G. Shavva
Ursolic and betulinic acids acids induce the processes of apoptosis. We tested the risk of osteoporosis induction under influence of triterpenoids, since they can interact with glucocorticoid receptors. In experiments on oviriectomized rats we found that acids do not show any negative influence on percentage of mineral components in the femur and have cholesterol-lowering action under experimental conditions.