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On-chip quantification of miRNA using digital droplet PCR
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

High-throughput analysis of protein formulations by DLS
Sophia Kenrick and Daniel Some

High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1

Digital microphotographic atlas of the adult zebrafish cerebellum
Kristen Lee, Megan Landy, Ashton Cline, Christopher Corbo, and Zoltan Fulop

Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.

Analytical Pipetting of Serum
John Thomas Bradshaw, PhD, Leah Flumerfelt, Richard H. Curtis, PhD, Rachel Parshley

Many types of chemical and biological analyses are based upon analytical techniques involving accurate delivery of liquid components. Pipetting tools have become commonplace especially in many biological and pharmaceutical laboratories. While these tools are familiar to many, their operational differences when pipetting different types of solutions are often over-looked.

Targeted cancer therapy based on blocking the expression of genes and small doses of oxaliplatin.
Bavykin A.S.1, Korotaeva A.A.1, Poyarkov S.V.2, Syrtsev A.V.1, Karpukhin A.V.1

The major drawbacks of the prescribed chemotherapy, are still remain the significant side effects and drug - resistance. Even targeted chemotherapy by means of specific antibodies do not always help to solve these complications. Our purpose was to identify potential biological targets associated with the development of drug resistance and to develop a specific method of suppressing the viability of cancer cells exposed to low doses of standard chemotherapy.

Cloud Point Extraction of Metal Oxide Nanoparticles in Water Samples Identified by Raman Spectroscopy and Quantified by Atomic Absorption Spectroscopy
Yanxiao Ma, Dr. Andrew F. Callender

Nanoparticles that are widely used in commercial products pose a threat to human health and the environment. This project proposes a cheaper and more effective method to identify and quantify metal oxide nanoparticles in water samples.

Cloud Point Extraction of Metal Oxide Nanoparticles in Water Samples Identified by Raman Spectroscopy and Quantified by Atomic Absorption Spectroscopy
Yanxiao Ma, Dr. Andrew F. Callender

Nanoparticles that are widely used in commercial products pose a threat to human health and the environment. This project proposes a cheaper and more effective method to identify and quantify metal oxide nanoparticles in water samples.

New Sorbent from Agro-industrial Waste and its Potential Use in 17ß-Estradiol and 17α-Ethynylestradiol Removal
Suzimara Rovani*, Éder C. Lima, Renato Cataluña, Andreia N. Fernandes

In recent years, the increasing introduction of new chemicals in the market, and the development of more accurate analytical methods, added a variety of endocrine disruptors compounds (EDC). Even though they are found in very low concentrations (range of ng L-1) there is still a lack of knowledge about long-term risks of EDC for non-target organisms as well as for human health.

Droplet Microfluidics for 3D Epithelial Cell Culture
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan

The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.

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Showing Results 101 - 110 of 312
Scientific News
DNA Suggests All Early Eskimos Migrated from North Slope
First evidence to genetically tie all Inuit populations to Alaska's North Slope.
Odd Histone Helps Suppress Jumping Genes in Stem Cells, Study Says
Research reveals a basic mechanism by which epigenetics, or the control of inherited traits through means other than DNA, works.
The Genetics Behind Abdominal Pigmentation in Drosophila melanogaster
This study by researchers at the North Carolina State University provides evidence that genetic variation at a number of steps in regulatory, developmental, and transport pathways may contribute to natural variation in abdominal pigmentation.
NIH-funded Study Points Way Forward for Retinal Disease Gene Therapy
Benefits for Leber congenital amaurosis peak after one to three years, then diminish.
Study Points to Possible Treatment for Lethal Pediatric Brain Cancer
NIH-funded preclinical study suggests epigenetic drugs may be used to treat leading cause of pediatric brain cancer death.
Mutations in Two Genes Linked to Familial Pulmonary Fibrosis and Telomere Shortening
PARN and RTEL1 genes strengthen the link between lung fibrosis and telomere dysfunction.
Proteomics Identifies DNA Repair Toolbox
Max-Planck scientists identify protein profiles of DNA repair.
Diet Swap has Dramatic Effects on Colon Cancer Risk for Americans and Africans
New study confirms that a high fibre diet can substantially reduce risk.
Statement on NIH Funding of Research Using Gene-Editing Technologies in Human Embryos
Researchers modify the gene responsible for a potentially fatal blood disorder using CRISPR/Cas9 technology.
Scientists Identify Key Receptors Behind Development of AML
Blocking ITIM-receptor signaling in combination with conventional therapies may represent a novel strategy for AML treatment.
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