|High Content Image Analysis using a Laser Scanning Microplate Cytometer|
Paul Wylie, Ben Schenker, Sarah Payne and Andrew Goulter
Microscope-based high-content instruments offer high optical resolution, however, the limited field of view afforded by their objective lenses can mean lengthy read times for some assays, especially where multiple image capture per well is required. Wherever possible, only a small percentage of the total number of cells present in a test well are analysed to keep plate read times at a minimum, which may not always be ideal. Microplate laser- scanning cytometers, such as TTP LabTech’s Acumen® eX3
|A genome-wide loss of function screen identifies new genes required for lung cancer cell proliferation|
PINNA G, ARAUJO N, MOROZOVA N, DETREZ MA & HAREL-BELLAN A
RNA interference (RNAi) provides an experimental tool for functional genomics analysis. We screened a genome-wide RNAi library to identify new genes involved in lung cancer cell proliferation. 72 % from the 257 genes identified were involved in general gene expression processes, 16 % were of unknown function or unrelated to proliferation, and 12% consisted of uncharacterized genes. These last sets of genes provide potential novel targets for lung cancer treatment.
|Specific Ligand-Based Depletion of Human Albumin and IgG in the Presence of Denaturants|
Todd Sanderson, Hongshan Li, Brian Miller, Laura Alionte, and Lisa Bradbury
Poster data highlights the advantages of a ligand-based depletion method that functions in the presence of urea and is highly specific, disposable, cost effective, and flexible.
|A New Disposable, Specific Ligand-Based Method for Depletion of Albumin and IgG From Serum and Plasma|
Laura Alionte, Allison Miller, Cal Messing, Tao Hu, and Lisa Bradbury
Poster shows the advantages of a ligand-based depletion method that is highly specific, disposable, cost effective, and flexible. Pall’s Enchant Multi-Protein Affinity Separation Kit is a strong addition to the product choices for scientists engaged in biomarker discovery and proteomics research.
|Expression of stress response protein GRP78 is associated with the development of castration-resistant prostate cancer|
Llana Pootrakul, Anirban P. Mitra, Ram H. Datar, Shan-Rong Shi, Jie Cai, Debra Hawes, Susan G. Groshen, Amy S. Lee, Richard J. Cote
This study investigated the role of the GRP78 in prostate cancer progression and the development of castration-resistant prostate cancer, where cancer cells continue to survive despite the stress of an androgen-starved environment.
|Application of genetic programming in analysis of quantitative gene expression profiles for identification of nodal status in bladder cancer|
Anirban P. Mitra, Arpit A. Almal, Ben George, David W. Fry, Peter F. Lenehan, Vincenzo Pagliarulo, Richard J. Cote, Ram H. Datar, William P. Worzel
Nodal involvement in bladder cancer is an independent indicator of prognosis. This study employed an iterative machine learning process called genetic programming on quantitative expression values of 70 genes to classify primary urothelial carcinoma samples into those associated with or without nodal metastasis. The generated rules showed a strong predilection for ICAM1, MAP2K6 and KDR resulting in gene expression motifs that cumulatively suggested a pattern ICAM1>MAP2K6>KDR for node positive ca
|New Protein Biomarkers for Histopathological Classification of Breast Cancer |
Brozkova Kristyna, Knoflickova Dana, Bouchal Pavel, Nenutil Rudolf and Vojtesek Borivoj
We used Ciphergen technology based on SELDI TOF-MS (Surface Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry) for analysis of protein expression profiles of 105 breast cancer tissue samples. The aim of this study was the identification of single proteins or protein patterns specific for different tumour subgroups, which should take advantage of the biomarker as an alternative to commonly used diagnostic and prognostic characteristics.
|The Silencing of Multidrug Resistance-Associated Protein 5 by siRNA Complexes|
M.Malinen, E.Mannermaa, T.Ryhänen, E.Kuusela, M.Häkli, M.Yliperttula and A.Urtti
The purpose is to study the role of multidrug resistance-associated protein 5, MRP5 in drug metabolism in human retinal pigment epithelium (RPE). RPE forms the outer part of blood-retinal barrier (BRB) which restricts movements of solutes from systemic bloodstream to the neural retina. The efflux protein, MPR5 is expressed in RPE but its functions are mainly unknown. SiRNA will be tested as a tool to clarify the role of MRP5.
|Two-Dimensional Molecular Profiling of Multiple Myeloma |
Zelena Jana, Konecna Hana, Zdrahal Zbynek, Penka Miroslav and Hajek Roman
We have compared two different solubilization buffers, we also evaluated protein precipitation with ethanol and optimized 2-DE conditions for human myeloma proteins.