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Automated Genomic DNA QC Ensures High Quality Data from Downstream Workflows
Arunkumar Padmanaban, Ruediger Salowsky, Delphine Rabiller and Donna McDade Walker

The success of several genomics study depends primarily on the quality of starting material, which in most cases is the genomic DNA. The quality and quantity of the extracted genomic DNA affects the downstream applications like microarray studies, library constructions and gene expression studies.

High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Arunkumar Padmanaban, Ruediger Salowsky, Adam Inche

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.

FDG PET/CT Utility in Gynecologic Malignancies: A comprehensive review of anatomy, pathways of metastatic spread and scan findings
Nicholas Plaxton, MD, Aruna Polsani, MD, Raghuveer Halkar, MD, Karen Godette, MD, Barron, Bruce, MD

Our objective was to review the five major gynaecologic cancers (cervical, ovarian, uterine, vaginal, and vulvar) and demonstrate the role of FDG PET/CT in diagnosis, surveillance, FIGO staging and treatment strategy.

FDG PET/CT Characteristics of Adrenal Benign and Malignant Lesions
Nicholas Plaxton, MD, Raghuveer Halkar, MD, Bruce Barron, MD

We selected FDG PET/CT cases with strong key representative findings to help illustrate benign and malignant adrenal lesions. Tabular review of PET SUV values, Hounsfield units and lesion size in the different cases will be discussed.

Hot Start dNTPs – Pushing the Limits of PCR
Tony Le, Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev

Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.

Mohamad Al-shammari wins Poster Award at System Biology Europe 2012
Mohamad Al-Shammari

Mohamad Al-shammari wins Poster Award at System Biology Europe 2012

Improved Ligation Specificity with Chemically Modified Ligation Components
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.

Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi

Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.

Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation
Wu-Bo Li and Michael Goldblatt

Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.

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Showing Results 111 - 120 of 285
Scientific News
Personalized Screening for Ovarian Cancer
With 60% of women diagnosed with ovarian cancer dying within five years of diagnosis there has been considerable efforts to try to detect the disease at an earlier stage.
New Genetic Form of Obesity and Diabetes Discovered
Scientists have discovered a new inherited form of obesity and type 2 diabetes in humans.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
Epigenetic Switches that turn Stem Cells into Blood Vessel Cells Uncovered
Researchers at the University of Illinois have identified a molecular mechanism that directs embryonic stem cells to mature into endothelial cells.
Study Finds Addition of Epigenetic Data Improves Predictions of Complex Traits
Researchers reported that combining genetic and epigenetic associations can bolster phenotypic predictions.
Expanding the DNA Alphabet: 'Extra' DNA Base Found to be Stable in Mammals
A rare DNA base, previously thought to be a temporary modification, has been shown to be stable in mammalian DNA, suggesting that it plays a key role in cellular function.
Stopping a Single Enzyme Could Help Treat Leukemia
EPFL scientists show how inactivating a single enzyme could effectively eradicate an aggressive form of leukemia. The principles could apply to other cancers as well.
Evo-Engineered CRISPR-Cas9s Hit More Gene-Editing Targets
Scientists have engineered a more effective CRISPR- Cas9 system paving the way for more advanced applications.
Cell that Replenishes Heart Muscle Found by UT Southwestern Researchers
Researchers devise a new cell-tracing technique to detect cells that do replenish themselves.
Researchers Find Molecular Mechanisms within Fetal Lungs that Initiate Labor
Biochemists found that steroid receptor coactivators 1 and 2 (SRC-1 and SRC-2) proteins control genes.
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