|Accurate Determination of Moisture Content of Soft Contact Lenses by Near-Infrared (NIR) Spectroscopy|
Dr. Hari Narayanan, Dr. Keith Freel
NIR spectroscopy was used to determine moisture content in soft contact lenses to and accuracy of 1%. Various sample presentation methods were used and the results were compared.
|Investigation of Chemical Contaminants in Soils Following Superstorm Sandy|
Amy C Mandigo*, Dana J DiScenza*, Alison R Keimowitz†, and Neil Fitzgerald*
Investigation of Chemical Contaminants in Soils Following Superstorm Sandy
|Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system|
Anne F Hammerstein, Diana Caracino, and Paul Wylie
The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.
|Combining low and high volume liquid handling capabilities for ADME screening|
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk
In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.
|Does the increase of exosomal microRNAs reflect an activated immune system in melanoma?|
Nina Koliha, Florian S. Dreyer , Jochen Dindorf , Andreas Bosio, Andreas S. Baur , and Stefan Wild
Cutaneous malignant melanoma is a form of skin cancer that accounts for 65% of skin cancer-related deaths. The incidence increases continuously, and while early detection leads to nearly 100% survival rates, the mortality raises to greater than 80% for patients with advanced disease.
|Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies|
W. John Martin
There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.
|Novel Nucleosomics Serum Biomarkers for the Detection of Colorectal Cancer|
S Holdenrieder, Y Dharuman, J Standop, M Herzog, N Trimpop, K Hettwer, K Simon, Steffen Uhlig and Jake Micallef
As colorectal cancers are often detected at late tumor stages when prognosis of the patients is poor, powerful blood-based biomarkers are needed for earlier detection. In recent years it has become clear that epigenetic changes to chromatin and its constituent nucleosomes are associated with carcinogenesis and elevated levels of nucleosomes are present in the blood of cancer patients.
|Fingerprinting analytical strategy used for authentication of honey|
Oana Romina DINCAa,b, Roxana Elena IONETEa*, Raluca POPESCUa, Radu TAMAIANa, Diana COSTINELa, Gabriel Lucian RADUb
The goal of the current study is mainly to establish potential origin markers, and not to build a model for prediction. This research provides a database for comparison of honey samples from Romania with other honey samples from the world. Data on d13C, d18O and d2H of honey measured by IRMS together with the stable isotopes (deuterium by SNIF-NMR and carbon 13 - IRMS) in ethanol from fermented honeys were reported.
|A novel DNA amplification method based on silicon microring resonator for rapid detection of genetic alteration in the point-of care (POC) field of cancers|
Nucleic acid testing plays a critical role in molecular diagnosis[1-2]. Due to the low amounts of DNA obtained in clinical samples, the amplification of DNA is an essential step in most NAT methods .