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A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

Mutation Induction in Sucrose Synthase 1 to Study Cold Acclimation in Winter Wheat
Rita Armoniene, Gintaras Brazauskas

Sucrose synthase (Ss) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose being one of the main enzymes of carbohydrate metabolism. The objectives of this study were: to create a TILLING population in winter wheat; to identify new alleles of Ss1 gene; to compare relative expression of Ss1 in leaves and crowns of mutant versus wild type plants during cold acclimation.

Characterization of the prehaustorial resistance against leaf rust (Puccinia triticina f. sp. tritici) in Einkorn (Triticum monococcum) by massive analysis of cDNA ends (MACE)
Albrecht Serfling1,2, Sven Templer1,3, Dragan Perovic1, Frank Ordon1

Triticum monococcum, a valuable source for horizontal resistance against P. triticina was analyzed microscopically and by transcriptional profiling. MACE showed the increased expression of chitinases, kinases, peroxidases and pathogenesis related genes in the first 8 hai. The high number of differentially expressd tags and the knowledge about SNPs facilitates in silico mapping and the development of polymorphic markers which may accelerate the transfer of this prehaustorial resistance

Genetic progress in the Romanian triticale breeding program
Ittu Gheorghe*, Saulescu Nicolae, Ittu Mariana, Mustatea Pompiliu and Marinciu Cristina

Since 1984 yield raised up to 43 kg / ha¹ and year¹ or 0.74 % /year¹ this progress being achieved mainly by improving fertility of spikes, plumpness of kernel, the test weight and introduction of short straw RhtB1b (Rht1) and Ddw1 (Hl) genes. In order to improve the yield stability, under predictions of global climatic changes, improvement of genetic diversity to powdery mildew, leaf and yellow rusts, virus and spike diseases and also for pre-harvest sprouting are still demanded.

Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

A non-destructive method to assess changes in the biomass of small-grain cereals under field conditions
D. Villegas1, J. Lozano2 and C. Royo1

The poster describes a non-destructive method to assess biomass based on remote sensing techniques. This method is suitable for field phenotyping and it is useful to assess biomass evolution as well as to estimate radiation use efficiency.

Characterization of nitrogen use efficiency in a winter wheat collection
István Monostori, Tamás Árendás, Fruzsina Szira, Gábor Galiba, Borbála Hoffmann, Attila Vágújfalvi

The first-year dataset of the three year experiment has been analysed. To find out, which genotype is recommended for intensive and which one is for extensive cultivation, the performances of the varieties were characterized under low and also under favourable nitrogen supply. The varieties were also ranked based on the increased level of their performance due to the nitrogen supply. Correlations between the measured phenotypic data and the NUE parameters were also calculated.

Genetic integrity of rice seeds during ageing and gene bank storage by SSR analysis
I. O. Daniel, K. A. Adeboye, O. W. Adabale, E. S. Aladele and A. Boerner

Rice seeds loose germination capacity with ageing. Loses in genetic integrity was found to occur during artificial ageing at a time when percentage germination declined to 54%.

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Showing Results 111 - 120 of 320
Scientific News
Gene Therapy for Metabolic Liver Diseases
Researchers have tested gene therapy in pigs from hereditary tyrosinemia type 1, with corrected liver cells being transplanted into the diseased liver.
Gene Terapy for Muscle Wasting Developed
New gene therapy could save millions of people suffering from muscle wasting disease.
Gene-Editing 'Toolbox' Targets Multiple Genes Simultaneously
Researchers have designed a system that modifies, or edits, multiple genes in a genome at once while minimising unintentional effects.
Discovering the First Farmers
Genetic analyses reveal a collection of highly distinct groups in the Near East and Europe at the dawn of agriculture.
Fighting Cancer Through Protein Pathways
Researchers have found a new drug target within a protein production pathway critical to regulating growth and proliferation of cells.
Mutations in DNA-Repair Genes Found in Advanced Prostate Cancers
New findings indicate that nearly 12% of male advanced prostate cancer sufferers have inherited mutation in DNA-repair genes.
Ice Bucket Challenge Instrumental in Gene Discovery
Donations from the ALS Ice Bucket Chellenge allowed for the largest-ever study of inherited ALS, which identified a new ALS gene.
Triple-Action Therapy Patch Shows Promise
Patch that delivers drug, gene, and light-based therapy to tumor sites shows promising results in mice.
Cancer Gene-Drug Combinations Ripe for Precision Medicine
The study aims to expand the number of cancer gene mutations that can be paired with a precision therapy.
Targeting BRAF Mutations in Thyroid Cancer
Treating metastatic thyroid cancer patients harboring a BRAF mutation with vemurafenib showed anti-tumor activity in a third of patients.
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