|Hot Start dNTPs - A Novel Tool for Controlled Nucleotide Incorporation in PCR|
Tony Le, Elena Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev
PCR is a widely used scientific tool employed by a variety of applications. Various Hot Start technologies have already been developed using modified PCR components to increase specificity of a reaction. Recently developed CleanAmpTM dNTPs are modified nucleoside triphosphates with a thermolabile 3’-tetrahydrofuranyl protecting group that is released at higher temperatures. These modified dNTPs prevent low temperature primer extension, which can often be a significant problem in PCR. At higher t
|Gene List Significance Index (GLSI) improves our method High Performance Chip Data Analysis dramatically - Quantifying the quality of different lists of analyzed significant genes|
Joachim R. Grün (1), Andreas Grützkau (1), Marta Steinbrich-Zöllner (3) Thomas Häupl (2), Ria Baumgrass (1), Jochen Sieper (3), Gerd-Rüdiger Burmester (2), Andreas Radbruch (1)
Our gene expression profiling strategy High Performance Chip Data Analysis (HPCDA) was improved with a method for quantifying the quality of different gene lists (GLs) with the new Gene List Significance Index (GLSI). With GLSI it is possible to decide which of two different extracted GLs has highest fraction of true positives, of high fold change or low p-value genes. With GLSI we could empirically optimize HPCDA-Score for ranking genes.
|A simple, fast and quantitative single-step dead-cell indicator for flow cytometry|
Jixiang Liu, Jolene Bradford, Chris Langsdorf
We have evaluated a series of new compounds for dead cell stain and identified a new product, SYTOX® AADvancedTM dead cell stain, which demonstrates improved properties over 7-AAD. These properties make the SYTOX® AADvancedTM dead cell stain a simple, fast and quantitative single-step no-wash dead-cell indicator as well as ideal for use in multicolor application requiring DNA content.
|Protein array-based screening of autoantibody signatures|
Zingaretti C., Arigò M., Colombatto P., Brunetto M., Muratori L., Bonino F., Bianchi F., Pagani M., De Francesco R., Abrignani S., Bombaci M.
The evidence for an association between autoimmune diseases and chronic HCV infection has been clearly established. To this aim, a protein array was employed to analyze serum samples of HCV patients w/wo autoimmune complications, of patients with autoimmune hepatitis but not infected with HCV and of healthy donors as controls. A panel of autoantigens able to discriminate among the three groups of patients was identified for potential use as biomarkers.
|PROTEOME WIDE PLASMA PROFILING USING ANTIBODY SUSPENSION BEAD ARRAYS|
Maja Neiman, Ulrika Igel, Burcu Ayoglu, Kimi Drobin, Mathias Uhlén, Peter Nilsson and Jochen M. Schwenk
A newly developed antibody suspension bead array assay allows for a systematic and high-throughput plasma profiling. This microtiter based assay uses antibody-coupled beads for a multiplexed analysis of minute amounts of directly labelled samples. The key requirement of a?nity reagents towards all human proteins is met by the Human Protein Atlas project.
|Inhibition of DNA methylation does not overcome docetaxel resistance in human breast cancer cells|
Kastl L, Brown I, Schofield A
DNA mehtylation can lead to chemotherapy resistance in cancer. The aim of this study was to investigate the role of DNA methylation in docetaxel-resistant human breast cancer cells. Whereas the DNA methylation machinery is altered in docetaxel-resistant human breast cancer cells compared to docetaxel-sensitive human breast cancer cells, resistance to docetaxel could not be reversed using the DNA methylation inhibitor decitabine.
|MitoProd patented technology for RNA manufacturing and its novel circular interfering RNA|
Jérome Lacoste*, Guillaume Plane*, and Jean-Paul Di Rago**
Here is a description of MitoProd patented technology for RNA manufacturing, permitting a recurring production of RNA in industrial quantities. This technology enables the production of custom RNA at a gram scale, 99 % full length and 95% pure. MitoProd has also designed a new class of interfering RNA called ciRNA®, which have improved features such as RNAses resistance and an increased in vivo efficiency compared to siRNAs.
|Software for Genomic/Epigenomic Research|
A.S.Tanas, V.V.Shkarupo, E.B.Kuznetsova, D.V.Zaletayev, V.V. Strelnikov
We present two computer programs: 1) AIMS in silico - a suggestion tool for Amplification of Intermethylated Sites experimental design and results analysis; and 2) PeakPick – a tool to view & analyse capillary electrophoregrams Our computer software is intended to standardize AIMS applications and to turn it into one of the principal approaches towards cancer methylomes studies as well as towards diagnostics in oncology, including early screening.
|MicroRNA-23b negatively regulates urokinase and c-met and inhibits migration of human hepatocellular carcinoma cells.|
Salvi A, Sabelli C, Moncini S, Venturin M, Arici B ,Riva P, Portolani N, Giulini SM, Barlati S and De Petro G.
By bioinformatics we predicted that miR-23b could recognize two sites in the 3’ UTR of uPA (urokinase-type plasminogen activator) and four sites in the 3’ UTR of c-met (hepatocyte growth factor receptor). miR-23b transfections in SKHep1C3 caused uPA and c-met decreased and migration and proliferation inhibition of SKHep1C3; anti-miR-23b transfection in human fibroblasts upregulated uPA and c-met. uPA and c-met shared a common microRNA that negatively regulates their expression.