Posters

Mohamad Al-shammari wins Poster Award at System Biology Europe 2012

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.

Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.

Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.

Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.

Copy number changes under the form of deletions and duplications are known to be involved in numerous human genetic disorders. Moreover, each individual’s genome embodies several copy number polymorphisms of various sizes which are thought to contribute to normal phenotypic variation and susceptibility to multifunctional disease.

This study applies ChIP-qPCR tp assess binding of transcription factor MYCN to miRNA cluster 17-92, to positive control target MDM2, and to a negative control target region.

Biomolecular protocols covering the whole cloning process were implemented in liquid handler robots. When compared to the manual approach, it was found that automation significantly speeds up HT cloning.

This poster addresses the reliability of qPCR data and its dependence on technical variations. The proposal is that constant volume of RNA extract can improve reliability of RT-qPCR.