|Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions|
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)
The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
|A non-destructive method to assess changes in the biomass of small-grain cereals under field conditions|
D. Villegas1, J. Lozano2 and C. Royo1
The poster describes a non-destructive method to assess biomass based on remote sensing techniques. This method is suitable for field phenotyping and it is useful to assess biomass evolution as well as to estimate radiation use efficiency.
|Genetic integrity of rice seeds during ageing and gene bank storage by SSR analysis|
I. O. Daniel, K. A. Adeboye, O. W. Adabale, E. S. Aladele and A. Boerner
Rice seeds loose germination capacity with ageing. Loses in genetic integrity was found to occur during artificial ageing at a time when percentage germination declined to 54%.
|Characterization of nitrogen use efficiency in a winter wheat collection|
István Monostori, Tamás Árendás, Fruzsina Szira, Gábor Galiba, Borbála Hoffmann, Attila Vágújfalvi
The first-year dataset of the three year experiment has been analysed. To find out, which genotype is recommended for intensive and which one is for extensive cultivation, the performances of the varieties were characterized under low and also under favourable nitrogen supply. The varieties were also ranked based on the increased level of their performance due to the nitrogen supply. Correlations between the measured phenotypic data and the NUE parameters were also calculated.
|Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip|
Javier Sanchez-Martin1, Simon Krattinger1, Margarita Shatalina1,2, Thomas Wicker1 and Beat Keller1*
Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip
|Intact Protein LC-MS: How to Overcome the Challenges?|
Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart
Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.
|On-chip quantification of miRNA using digital droplet PCR|
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2
miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
|High-throughput analysis of protein formulations by DLS|
Sophia Kenrick and Daniel Some
High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1
|Digital microphotographic atlas of the adult zebrafish cerebellum|
Kristen Lee, Megan Landy, Ashton Cline, Christopher Corbo, and Zoltan Fulop
Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.