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Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation
Wu-Bo Li and Michael Goldblatt

Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.

Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs
Pieter Mestdagh, Barbara D’haene, Jan Hellemans and Jo Vandesompele

Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.

Advanced Copy Number Variant Analysis with qbasePLUS 2
Barbara D’haene, Jo Vandesompele and Jan Hellemans

Copy number changes under the form of deletions and duplications are known to be involved in numerous human genetic disorders. Moreover, each individual’s genome embodies several copy number polymorphisms of various sizes which are thought to contribute to normal phenotypic variation and susceptibility to multifunctional disease.

ChIP-qPCR and qbasePLUS Jointly Identify a MYCN Activated miRNA Cluster in Cancer
Barbara D’haene, Pieter Mestdagh, Daniel Muth, Frank Westerman, Frank Speleman and Jo Vandesompele

This study applies ChIP-qPCR tp assess binding of transcription factor MYCN to miRNA cluster 17-92, to positive control target MDM2, and to a negative control target region.

Development of an Automated Platform for HT Cloning and Expression
Stefano Bonacci; Sara Iozzi; Scilla Buccato; Manuele Martinelli; John Telford; Domenico Maione; Roberto Petracca

Biomolecular protocols covering the whole cloning process were implemented in liquid handler robots. When compared to the manual approach, it was found that automation significantly speeds up HT cloning.

Volume-Related Inhibitors Standardization for Reverse Transcription Quantitative Polymerase Chain Reaction Experiments
Pascal Pugniere, Sebastien Banzet, Thomas Chaillou, Catherine Mouret and Endre Peinnequin

This poster addresses the reliability of qPCR data and its dependence on technical variations. The proposal is that constant volume of RNA extract can improve reliability of RT-qPCR.

Defining off-target cleavage in a pair of Zinc Finger Nucleases
K. Mukherjee, D. Carroll

This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.

Gene Expression Profiling: qPCR Toolkit for Quality Control
Švec D., Jacobsson J., Sjöback R., Kubista M.

TATAA Biocenter explain how they developed and optimized high-throughput gene expression qPCR with ValidPrime quality control, compensating for inter-run variations.

Cytotoxicity Screen of Mangiferin and its Major Metabolite Norathyriol in Human Tumor Cell Lines
Souza, J.R.R., Feitosa, J.P.A., Ricardo, N.M.P.S, Trevisan, M.T.S., Frei, E., Ulrich C.M., Owen, R.W.

Many natural products are available worldwide as potential chemoprotective agents against commonly occurring cancers, for example Mangiferin which has low bioavailability and is thought to be mainly available in the colon.

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Showing Results 51 - 60 of 224
Scientific News
500 Million Year Reset for the Immune System
A single factor can reset the immune system of mice to a state likely similar to what it was 500 million years ago, when the first vertebrates emerged.
Sequencing Identifies Gene Variant Responsible for Lupus
Research demonstrates it is feasible to identify the individual causes of lupus in patients by using DNA sequencing, allowing doctors to target specific treatments to individual patients.
Suspect Gene Corrupts Neural Connections
“Diseases of synapses” demo’d in a dish - NIH-funded study.
New Material Could Enhance Fast and Accurate DNA Sequencing
Nanopores in the material MoS2 sequence DNA more accurately, quickly and inexpensively.
Heart Molecule Discovery Could Lead to Effective Treatment for Heart Failure
Researchers have discovered a previously unknown cardiac molecule that could provide a key to treating, and preventing, heart failure.
Computer Model Reveals Cancer's Energy Source
Findings focused on the energy-making process in cancer cells known as the Warburg Effect.
Immune Cells get Cancer-Fighting Boost From Nanomaterials
Yale researchers used bundled carbon nanotubes to incubate cytotoxic T cells.
Epigenetic Study Bolsters Alzheimer's Understanding
The current study found that chemical modifications to DNA within the ANK1 gene are strongly associated with measures of neuropathology in the brain.
New Gene Editing Method Shows Promising Results for Correcting DMD
UT Southwestern researchers use CRISPR/Cas9-mediated genome editing technique.
New Ways to Treat Solid Tumours
An international team of scientists has shown that an antibody against the protein EphA3, found in the micro-environment of solid cancers, has anti-tumour effects.
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