|FDG PET/CT Characteristics of Adrenal Benign and Malignant Lesions|
Nicholas Plaxton, MD, Raghuveer Halkar, MD, Bruce Barron, MD
We selected FDG PET/CT cases with strong key representative findings to help illustrate benign and malignant adrenal lesions. Tabular review of PET SUV values, Hounsfield units and lesion size in the different cases will be discussed.
|Hot Start dNTPs – Pushing the Limits of PCR|
Tony Le, Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev
Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.
|Mohamad Al-shammari wins Poster Award at System Biology Europe 2012|
Mohamad Al-shammari wins Poster Award at System Biology Europe 2012
|Improved Ligation Specificity with Chemically Modified Ligation Components|
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe
Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.
|Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA|
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi
Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.
|Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation|
Wu-Bo Li and Michael Goldblatt
Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.
|Simultaneous RT-qPCR Measurement of 1718 Long Non-Coding RNAs|
Pieter Mestdagh, Barbara D’haene, Jan Hellemans and Jo Vandesompele
Massively parallel RNA-sequencing revealed that the human genome is pervasively transcribed, resulting in the production of thousands of non-coding RNA transcripts.
|Advanced Copy Number Variant Analysis with qbasePLUS 2|
Barbara D’haene, Jo Vandesompele and Jan Hellemans
Copy number changes under the form of deletions and duplications are known to be involved in numerous human genetic disorders. Moreover, each individual’s genome embodies several copy number polymorphisms of various sizes which are thought to contribute to normal phenotypic variation and susceptibility to multifunctional disease.
|ChIP-qPCR and qbasePLUS Jointly Identify a MYCN Activated miRNA Cluster in Cancer|
Barbara D’haene, Pieter Mestdagh, Daniel Muth, Frank Westerman, Frank Speleman and Jo Vandesompele
This study applies ChIP-qPCR tp assess binding of transcription factor MYCN to miRNA cluster 17-92, to positive control target MDM2, and to a negative control target region.