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Automated Genomic DNA QC Ensures High Quality Data from Downstream Workflows
Arunkumar Padmanaban, Ruediger Salowsky, Delphine Rabiller and Donna McDade Walker

The success of several genomics study depends primarily on the quality of starting material, which in most cases is the genomic DNA. The quality and quantity of the extracted genomic DNA affects the downstream applications like microarray studies, library constructions and gene expression studies.

High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Arunkumar Padmanaban, Ruediger Salowsky, Adam Inche

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.

FDG PET/CT Utility in Gynecologic Malignancies: A comprehensive review of anatomy, pathways of metastatic spread and scan findings
Nicholas Plaxton, MD, Aruna Polsani, MD, Raghuveer Halkar, MD, Karen Godette, MD, Barron, Bruce, MD

Our objective was to review the five major gynaecologic cancers (cervical, ovarian, uterine, vaginal, and vulvar) and demonstrate the role of FDG PET/CT in diagnosis, surveillance, FIGO staging and treatment strategy.

FDG PET/CT Characteristics of Adrenal Benign and Malignant Lesions
Nicholas Plaxton, MD, Raghuveer Halkar, MD, Bruce Barron, MD

We selected FDG PET/CT cases with strong key representative findings to help illustrate benign and malignant adrenal lesions. Tabular review of PET SUV values, Hounsfield units and lesion size in the different cases will be discussed.

Hot Start dNTPs – Pushing the Limits of PCR
Tony Le, Hidalgo Ashrafi, Sabrina Shore, Victor Timoshchuk, Natasha Paul, Richard Hogrefe, Inna Koukhareva, Alexandre Lebedev

Hot Start dNTPs are a distinct approach that employs modified nucleoside triphosphates with a thermolabile protecting group. This modification blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation.

Mohamad Al-shammari wins Poster Award at System Biology Europe 2012
Mohamad Al-Shammari

Mohamad Al-shammari wins Poster Award at System Biology Europe 2012

Improved Ligation Specificity with Chemically Modified Ligation Components
Sabrina Shore, Alexandre Lebedev, Elena Hidalgo Ashrafi, Gerald Zon, Natasha Paul, Richard Hogrefe

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.

Gene Expression from Pseudourine and 5-Methylcytidine Modified Messenger RNA
Jiehua Zhou, Maggie L. Bobbin, Julie R. Escamilla-Powers, Anton P. McCaffrey and John J. Rossi

Study objective was to develop methodologies for gram scale synthesis of messenger RNA (mRNA) for gene therapy applications, as well as scalable purification methods that yield highly expressed, persistent and non-toxic mRNA.

Random Homozygous Gene Perturbation (RHGP) as a Tool for Target Discovery and Validation
Wu-Bo Li and Michael Goldblatt

Random homozygous gene perturbation (RHGP) can identify and validate any host (cellular) gene target that directly causes a desired phenotype without requiring prior knowledge of the target. The central feature of RHGP is a unique lentiviral-based genetic element, known as a gene search vector (GSV) designed to interrogate the entire genome and identify target genes that cause the phenotype of interest.

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Scientific News
New Research Unlocks a Mystery of Albinism
A team led by Brown University biologists has discovered the way in which a specific genetic mutation appears to lead to the lack of melanin production underlying a form of albinism.
Study Finds Genetic Clue To Menopause-Like Condition In Young Women
NIH-funded research may also contribute to understanding normal menopause.
Therapeutic Strategy May Treat a Childhood Neurological Disorder
Researchers have identified a possible therapy to treat neurofibromatosis type 1 or NF1.
People May Inherit ‘Gut’ Bacteria That Cause Crohn’s Disease And Ulcerative Colitis
Discovery is another step toward prevention and treatment of 1.6 million Americans with inflammatory bowel disease.
Long Noncoding RNAs: A Novel Prognostic Marker in Older Patients with Acute Leukemia
This study describes a new marker that might help doctors choose the least toxic, most effective treatment for older patients with acute myeloid leukemia.
Proteins Drive Cancer Cells To Change States
When RNA-binding proteins are turned on, cancer cells get locked in a proliferative state.
Signaling Mechanism Could Be Target For Survival, Growth Of Tumor Cells In Brain Cancer
Non-canonical EGFR signalling shown to make glioblastoma tumor cells more resistant to chemotherapy treatment.
Study Sheds New Light on Relationship Between Personality and Health
Study examine the relationship between certain personality traits and the expression of genes.
New Way To Turn Genes On
Technique allows rapid, large-scale studies of gene function.
CSHL Team Finds a Way to Make shRNA Gene Knockdown More Effective
A powerful algorithm that improves the effectiveness of an important research technology.
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