|"In-vitro studies on hydroxamic acid- CT DNA binding" |
In vitro studies of five the interaction between five different derivatives of hydroxamic acids, N-phenyl 2,4dichloro phenoxybutyro, N-m-tolyl 2,4 dichlorophenoxyglutero, N-m-tolyl-4-chlorophenoxyaceto, N-m-chloro-phenyl-tertiarybutylbenzo and N-p-tolyl-iso-valero hydroxamic acids and calf thymus DNA was investigated under simulated physiological condition.
|Efficacy of Gene Therapy in Restoring Vision in Leber's Congenital Amaurosis|
The research reviews current knowledge of the genetic causes of Leber's congenital amaurosis (LCA) and the development of a gene therapy intervention for LCA. The efficacy as well as the limitations of this form of treatment are evaluated in detail and recommendations are made for the future directions of gene therapy for Leber's congenital amaurosis.
|METHOD DEVELOPMENT FOR THE SIMULTANEOUS DETERMINATION OF METHYLMERCURY AND INORGANIC MERCURY IN SEAFOOD|
Sergio Carneado, Ariane V. Zmozinski, Carmen Ibáñez-Palomino, Àngels Sahuquillo, José Fermín López-Sánchez, Márcia M. da Silva
This work reports the method development for the simultaneous determination of methylmercury (MeHg+) and inorganic mercury (iHg) species in seafood. The study focused on the extraction and quantification of MeHg+ by liquid chromatography coupled to on-line UV irradiation and cold vapour atomic fluorescence spectroscopy (LC-UV-HG-AFS), using HCl 4 mol L-1 as extractant agent.
|Microextraction techniques combined with X-ray fluorescence spectrometry for determination of different metal ions in water|
Karina Kocot (a), Beata Zawisza (a), Eva Marguí (b), Ignasi Queralt (c), Rafal Sitko (a)
In present work new preconcentration procedures combining microextraction techniques and X-ray fluorescence spectroscopy were developed. Proposed methodologies are promising tools for multielemental analysis enabling detection of natural pollutants in different water samples.
|Quantitative Real-time PCR differentiation between Genetically Modified pollen and Genetically Modified flour in honey|
Eugénia de Andrade1*, Maria Lopes2, Maria Clara Fernandes1, Fátima Quedas2, Isabel Rodrigues1, Amélia Maria Lopes1
We investigated the ability of quantitative real-time PCR, using plasmids calibrants, together with the triploid maize seed endosperm and the haploid pollen, to get good estimates for the copy number of a transgene in relation to the copy number of a species specific gene as a mean to distinguish between pollen and flour from maize.
|Artificial Multi-Gene Expression Systems Design Service for Natural Compound Formation and Hetero Protein Complexes|
Bernauer, Hubert, Gregor Zipf and Josef Maier
Drug discovery of natural compounds drug development and drug target analyses as well as bioproduction can benefit from artificial genetic systems and constructions. The direction in which genes are to be developed is written in the genomes. Synthesis oriented genomic analyses of codon bias and tRNA adaption analyses are prerequisites for generating adaptive, highly functional genes.
|Characterization of Proteins and Protein Self Association (Oligomerization) with SEC-MALS|
Michel Terray, Mark Pothecary
In this poster, a series of proteins and their oligomers were characterized using SEC-MALS with UV and RI detection. The monomer molecular weights are measured and compared with those from column calibration. The molecular weights of their oligomers are also compared with column calibration and the differences explored.
|Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks|
Vishwa Nellore, Chris Dwyer
We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.
|Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection|
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn
We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.