Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
A Diversity of Genomes
New DNA from understudied groups reveals modern genetic variation, ancient population shifts.
Gene Could Reduce Female Mosquitoes
Virginia Tech researchers have found a gene that can reduce female mosquitoes over many generations.
Improving Crop Efficiency with CRISPR
New study of CRISPR-Cas9 technology from Virginia Tech shows potential to improve crop efficiency.
Examining mtDNA May Help Identify Unknown Ancestry That Influences Breast Cancer Risk
Researchers studying mtDNA in a group of triple negative breast cancer patients found that 13 percent of participants were unaware of ancestry that could influence their risk of cancer.
Bacteria Use Ranking Strategy to Fight Off Viruses
Researchers have explained why microbes store virus confrontation information sequentially, with most recent attacks first.
Gene Therapy Technique May Help Prevent Cancer Metastasis
Gene-regulating RNA molecules could help treat early-stage breast cancer tumors before they spread.
Enhancing Antibiotics to Defeat Resistant Bacteria
Scientists enhance ability of antibiotics to defeat resistant types of bacteria using molecules called PPMOs
The Genetics of Blood Pressure
Researchers have identifed areas of the genome associated with blood-pressure including 17 previously unknown loci.
Mosquito Genetics Determine Tastes
Study reveals mosuito's preference for human versus animal biting is determined by genetics.
Quadruple Helix DNA Aids Cancer Therapies
Researchers have identified the role that a four-stranded version of DNA may play in the role of cancer progression.
Scroll Up
Scroll Down

BRD4 Bromodomain 1 Inhibitor Screening Using a Homogeneous Proximity Assay
Bookmark and Share

BioTek Instruments

Epigenetics refers to a dynamic set of processes that regulate transcription of the genome, some involving chemical modification of histone proteins. Histones associate with DNA to form nucleosomes, which bundle to form chromatin and chromosomes. Histones contain a flexible N-terminus tail, which protrudes from the nucleosome and is the site for epigenetic modifications. Modifications to amino acid residues in the histone tail include the addition and removal of functional groups, such as methyl and acetyl groups by specific enzymes. Other proteins affect epigenitic changes by reading whether the histone tail has been modified. A bromodomain protein is one such protein that recognizes and binds to the acetylated lysine residues in the histone tail. Inhibition of this protein: protein binding event is of major interest in some forms of cancer. This application highlights an HTS screening assay useful for studying the inhibition of bromodomain/histone binding. Continued interest in histone modifying proteins as druggable targets makes these types of assays an important focus area given the increased performance of the Synergy™ Neo HTS instrument. The assay is an extremely rapid and easy to perform biochemical assay with little optimization required aside from some initial instrumentation optimization. This assay was easily scaled to a 384-well format required for screening of small compound libraries.

Further Information

Related Content

DLReady Certification Awarded for Synergy 2 and Synergy HT Multi-Detection Microplate Readers
Awarded by Promega Corporation, this certification validates an instrument to the highest performance levels and standards for the Dual-Luciferase® Reporter Assay System.
Monday, November 06, 2006
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!