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Computational Model Finds New Protein-Protein Interactions
Researchers at University of Pittsburgh have discovered 500 new protein-protein interactions (PPIs) associated with genes linked to schizophrenia.
MicroRNA Pathway Could Lead to New Avenues for Leukemia Treatment
Cancer researchers at the University of Cincinnati have found a particular signaling route in microRNA (miR-22) that could lead to targets for acute myeloid leukemia, the most common type of fast-growing cancer of the blood and bone marrow.
Analysis of Dog Genome will Provide Insight into Human Disease
An important model in studying human disease, the non-coding RNA of the canine genome is an essential starting point for evolutionary and biomedical studies – according to a new study led by The Genome Analysis Centre (TGAC).
New Insights into Gene Regulation
Researchers have solved the three-dimensional structure of a gene repression complex that is known to play a role in cancer.
New Blood Test for The Earlier Diagnosis of Breast Cancer Spread
Researchers at University of Westminster have confirmed that a new blood test can detect if breast cancer has spread to other parts of the body.
GI Problems in Autism May Originate in Genes
Gene linked to autism lowers serotonin activity in mice, slows movement in gut.
Fructose Alters Hundreds of Brain Genes
UCLA scientists report that diet rich in omega-3 fatty acids can reverse the damage.
First Gene Therapy Successful Against Human Aging
American woman gets biologically younger after gene therapies.
Genetic Variants for Happiness Discovered
VU Amsterdam scientists have found a genetic overlap between happiness and depression.
DNA Barcodes Gone Wild
A team of researchers at University of Toronto’s Donnelly Centre and Sinai Health System’s Lunenfeld-Tanenbaum Research Institute (LTRI) has developed a new technology that can stitch together DNA barcodes inside a cell to simultaneously search amongst millions of protein pairs for protein interactions.
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BRD4 Bromodomain 1 Inhibitor Screening Using a Homogeneous Proximity Assay
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BioTek Instruments

Epigenetics refers to a dynamic set of processes that regulate transcription of the genome, some involving chemical modification of histone proteins. Histones associate with DNA to form nucleosomes, which bundle to form chromatin and chromosomes. Histones contain a flexible N-terminus tail, which protrudes from the nucleosome and is the site for epigenetic modifications. Modifications to amino acid residues in the histone tail include the addition and removal of functional groups, such as methyl and acetyl groups by specific enzymes. Other proteins affect epigenitic changes by reading whether the histone tail has been modified. A bromodomain protein is one such protein that recognizes and binds to the acetylated lysine residues in the histone tail. Inhibition of this protein: protein binding event is of major interest in some forms of cancer. This application highlights an HTS screening assay useful for studying the inhibition of bromodomain/histone binding. Continued interest in histone modifying proteins as druggable targets makes these types of assays an important focus area given the increased performance of the Synergy™ Neo HTS instrument. The assay is an extremely rapid and easy to perform biochemical assay with little optimization required aside from some initial instrumentation optimization. This assay was easily scaled to a 384-well format required for screening of small compound libraries.

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