Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Searching Big Data Faster
Theoretical analysis could expand applications of accelerated searching in biology, other fields.
Growing Hepatitis C in the Lab
Recent discovery allows study of naturally occurring forms of hepatitis C virus (HCV) in the lab.
Inciting an Immune Attack on Cancer Cells
A new minimally invasive vaccine that combines cancer cells and immune-enhancing factors could be used clinically to launch a destructive attack on tumors.
Reprogramming Cancer Cells
Researchers on Mayo Clinic’s Florida campus have discovered a way to potentially reprogram cancer cells back to normalcy.
Genetic Overlapping in Multiple Autoimmune Diseases May Suggest Common Therapies
CHOP genomics expert leads analysis of genetic architecture, with eye on repurposing existing drugs.
Surprising Mechanism Behind Antibiotic-Resistant Bacteria Uncovered
Now, scientists at TSRI have discovered that the important human pathogen Staphylococcus aureus, develops resistance to this drug by “switching on” a previously uncharacterized set of genes.
How DNA ‘Proofreader’ Proteins Pick and Edit Their Reading Material
Researchers from North Carolina State University and the University of North Carolina at Chapel Hill have discovered how two important proofreader proteins know where to look for errors during DNA replication and how they work together to signal the body’s repair mechanism.
Fat in the Family?
Study could lead to therapeutics that boost metabolism.
Tissue Bank Pays Dividends for Brain Cancer Research
Checking what’s in the bank – the Brisbane Breast Bank, that is – has paid dividends for UQ cancer researchers.
Scroll Up
Scroll Down

Efficient PCR from whole blood using Finnzymes High Performance PCR solution
Bookmark and Share

Labtech International

Finnzymes have developed a range of reagents,consumables and instrumentation to allow fastPCR in inhibiting conditions. Here we describean application for amplifying exogenous andhuman genomic DNA from whole blood withoutthe need for DNA purification, and use ofspecial buffers or complicated protocols.Finnzymes' solution for High Performance (HP)-PCR results in faster amplification with betteryields for raw blood samples as compared withconventional methods for PCR.

Finnzymes’ HP-PCR is a tripartite solution focused onspeeding up a PCR and providing marked improvementsin yield while maintaining high fidelity. The uniquecombination of our highly processive PhusionTM DNAPolymerases, quick PikoTM Thermal Cyclers and ultra-thinwalled UTWTM tubes provides considerable benefits to thespeed, yield, fidelity, specificity and robustness of PCR.Central to these improvements is our Phusion technology,which enhances activity by way of a double-strandedDNA-binding domain bound to a highly engineeredproofreading polymerase. This dsDNA-binding domainallows for ultra-robust reactions by tethering thepolymerase to the duplex DNA, driving much higherassociation constants, so the effects of conventionalinhibitors of PCR are significantly reduced1. Here wedescribe one such application, a simple protocol for robustamplification of DNA from whole blood. Whole blood isoften used in clinical diagnostics and as a DNA source forPCR tests. Unfortunately, Taq DNA polymerase, the mostcommonly used enzyme for PCR, is known to becompletely inhibited by even small quantities of wholeblood in the reaction2. Several strategies have been usedto overcome this difficulty, including the use of specializedpurifications, buffers and pretreatments3,4, but all of thesemethods have drawbacks, adding time and cost, and oftenproducing inconsistent results. As a consequence,purification of genomic DNA from whole blood remains acommon practice even though it adds considerable timeand expense to the test.

Further Information


Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!