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Nucleofection®: A Method to Stably Transfect C1R Cells With MHC Class I Genes
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Amaxa GMBH

Major histocompatibility complex (MHC) class I molecules are heterotrimeric cell surface glycoproteins found on most nucleated cells of the body displaying endogenously synthesized peptides. Cells displaying class I molecules with healthy host-derived peptide ligands remain unharmed while those presenting peptide ligands characteristic of infected or neoplastic cells become targets for the immune response. In humans the main MHC class I subfamilies are the Human Leukocyte Antigen (HLA) loci A, B and C, each of which has a large number of alleles. In this application note we describe the simple and efficient derivation of C1R cells stably expressing HLA-B18. This work is expected to provide us with a model system for examining self peptides from a given HLA-locus.

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