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Thursday, July 31, 2014
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Featured Products
Featured Product - Genotyping Services
Genotyping Services

Webcasts
Webcast - Tumour Regression after Intravenous Administration of Novel Tumour-targeted NanomedicinesTumour Regression after Intravenous Administration of Novel Tumour-targeted Nanomedicines
Christine Dufes, University of Strathclyde, speaking at Nanomedicine 2014.
Webcast - Extreme PCR:  Robust, Efficient Amplification in < 1 MinExtreme PCR: Robust, Efficient Amplification in < 1 Min
Carl Wittwer, University of Utah, speaking at Advances in qPCR and dPCR

Market Reports
Gene Expression Analysis Market by Technology, Consumables, Services & Applications - Global Forecast to 2018
MarketsandMarkets

Over the years, the gene expression analysis market, comprising of instruments, consumables, and services, has witnessed various technological advancements. These advancements have led to a growth in the number of gene expression applications.
Mass Spectrometry Market by Technology & Application - Global Trends & Forecast to 2018
MarketsandMarkets

The global mass spectrometry market witnesses high competitive intensity as there are many big and small firms with similar product offerings. These companies adopt various strategies to increase their market shares and to establish a strong foothold in the global market.
Biomarkers Market 2013 - 2020
Allied Market Research

Disease diagnostic application remains the key area of application that is expected to reach 6.1 $billion in 2020. However, application of diagnostic biomarkers in forensics and molecular diagnostics is catching up quite significantly. Molecular diagnostics is expected to grow at a CAGR of 17.6% from 2013 to 2020.
Analysis of Global Biochips Industry, 2012-2018
Axis Research Mind

The report ‘Analysis of Global Biochips Industry, 2012-2018’ reviews the latest biochips market trends with a perceptive attempt to disclose the near-future growth prospects. An in-depth analysis on a geographic basis provides strategic business intelligence for life science sector investments.
Biomarkers
RI Technologies

This report on focuses on the different types of biomarkers and their impact on various disease areas. The study also provides global market analysis for biomarkers by disease area and by prominent biomarker identification technologies.
Biomarkers in Drug Discovery - Integration in Early Stage Promotes Use of Companion Diagnostics to Optimize Therapeutic Outcomes
Global Data

The report describes the history of biomarkers and their role in fulfilling the unmet needs of the conventional drug discovery process. The drivers and barriers for the biomarkers market, along with the key issues faced in the application of biomarkers in the drug discovery process, are highlighted.
Life Science Software 2013: Markets and Opportunities
Biopharm Reports

This reports into in the life science field examines the use of specialist software and databases relating to the conduct of PCR, DNA sequencing, DNA microarray, flow cytometry, mass spectrometry and disease biomarker studies. The studies were carried out to assist software developers and vendors to identify market opportunities in these fields.
DNA Sequencing 2013: Market Developments, Growth Areas and Opportunities
Biopharm Reports

DNA sequencing is important in all areas of biological research and increasing demand for this technique is driving advances in this field, in the form of next-generation techniques. Today, laboratories use a number of different sequencing methods, however common goals include increasing throughputs and speeds, while reducing costs.
Life Science Consumables 2013: Markets and Opportunities
Biopharm Reports

Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have grown significantly in the last decade and by 2015 markets for these five areas are expected to exceed $50 billion, globally. In these and related fields, consumables are generally the second or third largest area in cost terms, after instrumentation.
qPCR Assay Trends 2013
HTStec

HTStec's qPCR Assay Trends 2013 report was published on 15 April 2013. This 63 page market report summarizes the results of HTStec's 3rd industry-wide global web-based benchmarking survey on real-time quantitative PCR (qPCR) assays and instruments carried out in March 2013.
Scientific News
Researchers Uncover New Cancer Cell Vulnerability
The research showed that telomerase-expressing cells depend upon a gene named p21 for their survival.
Capturing Cancer: A Powerful New Technique for Early Diagnosis
Researchers describe an innovative technique for early disease detection, which they call immunosignaturing.
No Extra Mutations in Modified Stem Cells, Study Finds
New results ease previous concerns that gene-editing techniques-used to develop therapies for genetic diseases-could add unwanted mutations to stem cells.
Patient-Specific Stem Cells and Personalized Gene Therapy
Patients’ own cells transformed into model for studying disease and developing potential treatment.
Significant Step Towards Blood Test for Alzheimer's
Scientists have identified a set of 10 proteins in the blood which can predict the onset of Alzheimer’s, marking a significant step towards developing a blood test for the disease.
Novel Insights into the Control of Gene Expression
The webinar, “Developing new insights into gene expression using RNA-Seq”, sees Dr Christopher Jones, Research Fellow at the Brighton and Sussex Medical School present novel findings from a recent RNA-Seq project performed by OGT.
Aging Contributes to Rapid Rates of Genomic Change
Signaling challenges for personalized medicine.
CRI Finds Key to Identifying, Enriching Mesenchymal Stem Cells
Biomarker enables researchers to accurately characterize the properties and function of MSCs in the body.
Saturated Fat Intake May Influence Person’s Expression of Genetic Obesity Risk
Limiting saturated fat could help people whose genetic make-up increases their chance of being obese.
Genetic Material Hitchhiking in Our Cells May Shape Physical Traits
Explaining the connection between genotype and phenotype must also consider genetic material that doesn’t come from an organism’s chromosomes at all.
Business News
QIAGEN Licenses Blood Cancer Biomarkers
Exclusive global license from University of Tokyo enables development of QIAGEN assays for SF3B1 mutations, including NGS gene panels, for blood cancers.
Cypher Genomics and Illumina Enter Agreement
Co-promotion agreement to facilitate genomic biomarker discovery.
ARUP Labs, UH Case Medical Center Sign Distribution Agreement for Novel HIV Test
DEEPGEN™HIV monitors the success of anti-HIV treatment by determining drug resistance and the ability of the virus to infect different cells based on deep genetic sequencing.
Novartis, Banner Institute Announce Alzheimer's Disease Collaboration
Two Novartis anti-amyloid investigational treatments will be studied in people with a genetic risk of developing Alzheimer's Disease.
NIH Funds $24M into Alzheimer’s Disease Genome Research
Scientists will analyze genome sequence data to identify gene risk, protective factors.
IROA Technologies, University of Florida Announce Biomarker Collaboration
Company will support the University of Florida in a collaborative search for biomarkers of liver cancer and other liver diseases.
Definiens Secures € 15 Million for Tissue Phenomics-based Cancer Diagnostics
Big data approach bridges the gap between genomics and personalized medicine.
Almac’s AADx Gene Expression Marker Attracts Significant Commercial Interest from Pharma
Evaluation in colorectal, breast and lung cancer to follow.
Inform Genomics and TD2 Announce Strategic Collaboration
Collaboration to provide inform genomics proprietary technology platform and capabilities to drug developers.
Intertek Expands San Diego Immunochemistry Center of Excellence
Intertek to expand its immunochemistry facility in San Diego with a move to a larger center of excellence.

Events
Enabling Genomic Technologies
11 Sep 2014
Enabling Genomic Technologies ...
Plant Genomics USA
11 Sep 2014
Plant Genomics USA ...
EMBO Workshop: Unravelling Bio
25 Sep 2014
EMBO Workshop: Unravelling Bio ...
European Antibody Congress 201
10 Nov 2014
European Antibody Congress 201 ...

ePosters
Characterization of the prehaustorial resistance against leaf rust (Puccinia triticina f. sp. tritici) in Einkorn (Triticum monococcum) by massive analysis of cDNA ends (MACE)
1 Julius Kuehn-Institute (JKI) Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, 06484 Quedlinburg, Germany 2 Interdisciplinary Center for Crop Pla

Triticum monococcum, a valuable source for horizontal resistance against P. triticina was analyzed microscopically and by transcriptional profiling. MACE showed the increased expression of chitinases, kinases, peroxidases and pathogenesis related genes in the first 8 hai. The high number of differentially expressd tags and the knowledge about SNPs facilitates in silico mapping and the development of polymorphic markers which may accelerate the transfer of this prehaustorial resistance
Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
(1) Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany; (2) Julius Kühn-

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
(1) Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany; (2) Julius Kühn-

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip
1Institute of Plant Biology, University of Zurich, Zurich, Switzerland. 2Current address: GeneData AG, Basel, Switzerland.

Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip
Intact Protein LC-MS: How to Overcome the Challenges?
Thermo Fisher Scientific

Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.
On-chip quantification of miRNA using digital droplet PCR
1. Life Science Technologies, Imec, Kapeldreef 75, Heverlee, Belgium 2. Advanced Technology Research Laboratory, Panasonic Corporation, Kyoto Japan

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
High-throughput analysis of protein formulations by DLS
Wyatt Technology Corporation

High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1
Digital microphotographic atlas of the adult zebrafish cerebellum
Wagner College

Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.
The Effects of Dimethylphthalate on the Viability and Development of Drosophila melanogaster
Wagner College

My research studies the effect of dimethylphthalate (DMP), a putative endocrine disruptor, on the viability and development of the model organism Drosophila melanogaster.
Analytical Pipetting of Serum
Artel

Many types of chemical and biological analyses are based upon analytical techniques involving accurate delivery of liquid components. Pipetting tools have become commonplace especially in many biological and pharmaceutical laboratories. While these tools are familiar to many, their operational differences when pipetting different types of solutions are often over-looked.
Application Notes
Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences
Tecan Group Ltd.

One of the challenges in life sciences research today is to discover methods for running key assays more quickly, more reliably and using lower volumes of reagents and sample, but still with improved sensitivity. One area where this particularly holds true is for traditional enzyme-linked immunosorbent assays (ELISAs), whose application provides a useful measurement of antigens, including cytokines and a host of other biomarkers.
Molecular indexing for improved RNA-Seq analysis
Bioo Scientific

Most modern methods for nucleic acid analysis require the use of enzyme processing, such as DNA polymerase reactions, in the sample preparation or measurement steps. Although necessary, these enzymatic steps introduce errors in the form of incorrect sequence and misrepresented copy number. NEXTflex™ qRNA-Seq™ Kit from Bioo Scientific adds no additional steps to the work flow, costs no more than a conventional library preparation kit and increases the precision of downstream analysis.
Purification of Genomic DNA from Saliva Using Oragene® Discover Collection Kits and the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling Workstation
Hamilton

In the following studies, we have demonstrated the high precision of the automated TruTip extraction technology in processing genomic DNA from low-volume saliva samples and its advantage over competitors.
Rapid, High Throughput Extraction and Purification of Genomic DNA from Whole Blood Using the Akonni TruTip® Extraction System on the Hamilton Microlab® STAR Liquid Handling System
Hamilton

This application note demonstrates the high precision of the automated extraction system in processing genomic DNA from low volume whole blood samples and its advantage over competitors.
Next Generation Sequencing Method for Illumina TruSeq DNA Sample Preparation Protocol on the Hamilton STAR
Hamilton

The purpose of the TruSeq sample preparation protocol is to add adapter sequences onto the ends of DNA fragments to generate multiplexed sequencing libraries. Good liquid handling is crucial for pipetting precise volumes for example for Agilent Bioanalyzer.
A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1)
BMG LABTECH

Post-translational histone modifications like acetylation play a pivotal role in the epigenetic regulation of transcription. Catalyzing the latter reaction HDACs affect various cellular processes especially cancerogenesis. Although the mechanism of starting cancerogenesis by epigenetic events is not clearly explained inhibition of HDACs has highlighted as a viable principle in cancer therapy.
A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
Bio-Rad Laboratories

In this study, the minichromosome maintenance (MCM) protein, MCM7, was selected as a model target gene to investigate the importance of appropriate reference gene selection and RNA sample quality as described by the MIQE guidelines.
Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA Microplate Reader
BMG Labtech

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of the FLUOstar OPTIMA multidetection microplate reader from BMG LABTECH.
High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.
Isolation and Purification of Genomic DNA and Viral RNA from Clinical Samples with Thermo Scientific KingFisher Flex
Thermo Fisher Scientific

The purification of DNA and RNA from clinical samples requires rapid and reliable processing of large quantities of samples. Automated purification of nucleic acids, proteins and cells with Thermo Scientific KingFisher Flex is a reproducible and high quality method, which produces excellent material for further analyses.
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