Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Understanding and Using Isothermal Gene Assembly for Synthetic Biology

Published: Tuesday, August 28, 2012
Last Updated: Tuesday, August 28, 2012
Bookmark and Share
Latest issue of IDT’s Decoded newsletter explains the fundamentals of artificial gene construction.

Integrated DNA Technologies (IDT) provides a unique insight into the fundamentals of isothermal synthetic gene assembly in this quarter’s edition of the company’s Decoded newsletter.

The technique, which was pioneered in 2009 by Daniel Gibson and colleagues at The J. Craig Venter Institute in Maryland, USA, allows several overlapping DNA fragments to be combined in a single reaction.

In this way, large synthetic DNA molecules many hundreds of kilobases long can be generated using a minimal number of enzymatic steps.

This limits potential errors due to repeated handling of samples and reagents, while simplifying the process by avoiding the need for multiple experimental setups.

The article in the latest issue of Decoded outlines the theory behind isothermal assembly, and provides advice for designing and creating synthetic gene constructs using the method.

Users can read more about the latest genomic trends, tips and research in the most recent issue of IDT’s quarterly newsletter, Decoded.

IDT has also released its new gBlock Gene Fragments, which simplify, speed up and reduce the cost of creating large, custom DNA constructs.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Dr Patricia Tavormina Named Winner of First IDT ISO 14001 Sustainability Award
Biodiversity research on methane sinks and global warming earns $14,001 in oligo credit.
Thursday, September 10, 2015
Bringing NGS to the Clinic: What Can We Expect?
Dr Elaine Mardis shares her views with IDT on the future of NGS and its role in personalized medicine.
Thursday, February 07, 2013
Scientific News
Gene That Lowers Heart Attack Risk Identified
Individuals with a rare twelve-letter deletion from a gene on chromosome 17 have significantly reduced non-HDL cholesterol levels and a 35% lower than average risk of heart disease.
"Sunscreen" Gene May Guard Against Melanoma
USC-led study reveals that melanoma patients with deficient or mutant copies of the gene are less protected from harmful ultraviolet rays.
Myeloid-Derived Suppressor Cells Play Role in Tumor Growth
Researchers at Baylor College of Medicine have reported a new mechanism that helps cancer cells engage myeloid-derived suppressor cells.
Transcription Factor Isoforms Implicated in Colon Diseases
UC Riverside study explains how distribution of two forms of a transcription factor in the colon influence risk of disease.
Roundup Impacts Gene Expression
Study published on the impact of low-dose toxicity of Roundup weed-killer on gene expression profiles.
US-India Collab Finds Molecular Signatures of Severe Malaria
Study may be a significant advancement in understanding the causes of severe malaria.
Big Data Can Save Lives
The sharing of genetic information from millions of cancer patients around the world could be key to revolutionising cancer prevention and care, according to a leading cancer expert from Queen's University Belfast.
Triple-Negative Breast Cancer Target Is Found
Researchers at UC Berkeley discover a target that drives cancer metabolism in triple-negative breast cancer.
Gene Expression Controls Revealed
Researchers have modelled every atom in a key part of the process for switching on genes, revealing a whole new area for potential drug targets.
Using Portable Nanopore DNA Sequencers to Combat Wildlife Crime
University of Leicester researchers aim to develop a test using DNA to identify species at crime scenes in as little as an hour.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!