Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

BGI Tech Develops Whole Exome Sequencing Analysis of FFPE DNA Samples to Accelerate Biomedical Research

Published: Friday, September 21, 2012
Last Updated: Friday, September 21, 2012
Bookmark and Share
Achieving optimization of FFPE DNA library construction with DNA down to 200 ng.

BGI Tech Solutions Co., Ltd., a subsidiary company of BGI, announced today that they have achieved whole exome sequencing analysis of total degraded DNA as low as 200 ng from formalin fixed paraffin embedded (FFPE) samples. This advancement enables researchers to efficiently uncover the genetic information from FFPE disease samples such as cancers and infectious diseases, with the advantages of high reliability, accuracy and fast turnaround time.

FFPE samples are the most common biological materials for disease diagnoses and clinical studies. Especially in cancer research, millions of FFPE archival cancer tissue samples provide an enormous and invaluable repository of information, which hold a wealth of data for the discovery of biomarkers, drug development as well as diseases diagnosis and treatment.

However, during the FFPE sample preparation and storage process, the effect of formaldehyde on nucleic acids is detrimental, which can induce modification of nucleotide molecules, such as DNA damage, DNA-protein cross-links (DPC), among others. This may lay problems for researchers to get enough high-quality DNA from these FFPE samples to comprehensively explore the genetic characteristics of diseases, especially for some rare tumors.

FFPE samples are a unique sample type with a lot of challenges, and researchers from BGI Tech have optimized the DNA extraction, library construction and sequencing pipelines of FFPE DNA samples. At present, DNA as low as 200 ng from FFPE samples can be used for whole exome sequencing. To insure the accuracy and quality of sequencing, researchers evaluated the FFPE DNA sequencing results and demonstrated that FFPE exome sequencing could maintain the equivalent accuracy and reliability with the normal DNA sample sequencing.

It is reported that ~85% of genetic diseases are related with exome variations. Whole exome sequencing is a robust innovative technique that selectively sequences the coding regions of a genome and can be used to identify novel genes associated with rare and common diseases such as cancer, diabetes, and obesity. However, currently traditional exome sequencing has higher requirement for the quality and the amount of input of DNA samples.

Zhao Lin, Director of Products R&D Department of BGI Tech, said, “Our whole exome sequencing technology with FFPE DNA sample is an important step toward better and quickly decoding the genetic information underlying FFPE diseases samples. I believe this advancement will strengthen the confidence of researchers in pharmaceutical and disease areas, especially when samples are limited. In order to accelerate biomedical research, we expect to conduct more FFPE sequencing projects with collaborators worldwide.”


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Growing Hepatitis C in the Lab
Recent discovery allows study of naturally occurring forms of hepatitis C virus (HCV) in the lab.
Signature of Microbiomes Linked to Schizophrenia
Studying microbiomes in throat may help identify causes and treatments of brain disorder.
Study Identifies the Off Switch for Biofilm Formation
New discovery could help prevent the formation of infectious bacterial films on hospital equipment.
Genetic Overlapping in Multiple Autoimmune Diseases May Suggest Common Therapies
CHOP genomics expert leads analysis of genetic architecture, with eye on repurposing existing drugs.
Fat in the Family?
Study could lead to therapeutics that boost metabolism.
Combo Tool
Joining molecular components expands ability to manipulate genes in specific cell types.
Team Identifies Structure of Tumor-Suppressing Protein
An international group of researchers led by Carnegie Mellon University physicists Mathias Lösche and Frank Heinrich have established the structure of an important tumor suppressing protein, PTEN.
Genes Associated With Improved Survival for Pancreatic Cancer Patients
Use of non-invasive liquid biopsies could predict in which patients the cancer could recur following surgery.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!