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Avacta selects Trinean’s DropSense96® for purified protein QC

Published: Monday, October 01, 2012
Last Updated: Monday, October 01, 2012
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Trinean announced that its DropSense96® droplet reader has been chosen by Avacta for High Throughput analysis of their purified Affimer proteins.

Avacta has established facilities capable of expressing and purifying 15,000 of their proprietary Affimer proteins every 6 weeks. These affinity reagents, which can be thought of as artificial antibodies, will be incorporated into very high complexity protein microarrays designed to revolutionize the discovery and validation of disease biomarkers. “The high complexity of the protein arrays means that the Affimers have to be produced to very high standards of purity and yield." said Paul Ko Ferrigno, Head of Discovery Technology at Avacta, "Our challenge is that quality control becomes a rate-limiting step when working at this high throughput .We currently have plates of purified Affimers stacked waiting for analysis by traditional methods. Once the Trinean DropSense96® is introduced into the workflow, quality control will become as automated as the purification protocols, allowing the development team to go from bacterial colony to purified protein of known concentration within 2 days, processing one thousand proteins at a time."

Philippe Stas, CEO of Trinean, added: "The decision by Avacta to use our DropSense96® droplet reader further validates our instruments to deliver high quality UV/Vis spectral absorbance data. The DropSense96® was designed to meet the requirements of protein purification labs by combining speed and minimal sample usage. Our unmatched large measurement range allows quantification of undiluted protein samples in real-time thereby significantly shortening the purification process.


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