Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

The Microplate Reader Durable Enough to Withstand Changes in Gravity

Published: Wednesday, October 17, 2012
Last Updated: Wednesday, October 17, 2012
Bookmark and Share
Studying the physiological effects of gravity fluctuations on plants, researchers at the University of Tuebingen in Germany use BMG LABTECH’s POLARstar microplate reader in parabolic flights.

It is known that light and gravity are the two most important factors in helping plants determine where top and bottom are. It is easy to eliminate light to see what effect it has on plants, but trying to investigate changes in gravity prove a little more difficult. By measuring calcium and hydrogen peroxide in plant cells with the durable POLARstar microplate reader, researchers show the effects of gravity on plants during consecutive parabolic flights.

During a parabolic flight, different stages of gravitation are obtained ranging from hypergravity (about 1.8no-gravity-polarstar.gif g) to microgravity (about 10-3 g). In one flight, 31 consecutive parabolas are flown. Novespace, a company from France has been performing these flights for over ten years. The gravitational effects of the parabolic flight can be seen in a simulated video (http://www.novespace.fr/fr,popup,parabole.html) and in a real video (http://www.youtube.com/watch?v=Lhu198E8z2U). Normal gravity is designated as 1g and on the ascent of the parabola the gravity goes to about 1.8 g (80% higher than normal). Then on the descent of the parabola when the experiments are performed, the period of microgravity lasts for about 22 seconds.

To investigate the signaling pathway from the stimulus (no gravity) to the response (e.g. activation or deactivation of the expression of certain genes) the level of two important second messengers, Ca2+ and hydrogen peroxide, were monitored. Two transgenic cell lines derived from Arabidopsis thaliana expressing either Cameleon (a calcium sensor) or HyPer (an H2O2 sensor) were used as well as a wild type. The calcium and hydrogen peroxide measurements were performed on a fixed POLARstar OPTIMA in kinetic mode during the whole time of the parabolic flight.

Results show that the calcium levels increased with microgravity and decreased afterwards, signifying an effect on calcium flux due to changes in gravity. Further parabolic flights with the POLARstar OPTIMA and with a new PHERAstar FS are in preparation. Some of the results can be found online (http://www.bmglabtech.com/customer-focus/parabolic-flights.cfm). Corresponding experiments were also recently performed at higher gravity levels with the robust POLARstar microplate reader. Using the Hyper-g centrifuge at ZARM (The Center of Applied Space Technology and Microgravity) in Bremen Germany, the entire unit has been routinely centrifuged up to 10g, further demonstrating the robustness of the instrumentation.  

The POLARstar microplate reader from BMG LABTECH is a multimode plate reader that can measure fluorescence, luminescence, absorbance, and fluorescence polarization. Its robust steel housing and perfectly engineered machinery allow it to perform even under extreme conditions such as high and low levels of gravity.

These experiments were financed by a grant from the Deutsches Zentrum für Luft- und Raumfahrt (DLR; 50WB0723). For more information about the Tuebingen research group please have a look here:
http://www.bbp.uni-tuebingen.de/index.php5?object=Content.ResearchTopics.GravitationBiology.

BMG LABTECH is a leading developer and global manufacturer of microplate reader instrumentation with a wide range of measurement methods. Microplate readers are used in the pharmaceutical and biotech industries, as well as in academic research establishments, for both basic research analysis and High Throughput Screening. BMG LABTECH focuses solely on microplate readers and offers the most diverse selection of optical detection systems in conjunction with integrated liquid handling equipment. For more information visit: www.bmglabtech.com

BMG LABTECH, bringing the future of microplate reader technology to you today.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
The Spice of Life
Scientists discover important genetic source of human diversity.
Cytoskeleton Crew
Findings confirm sugar's role in helping cancers survive by changing cellular architecture.
Removing Race from Human Genetic Research
A group of scientists are urging their colleagues to take a step forward and stop using racial categories when researching and studying human genetics.
Biomarker for Recurring HPV-Linked Oropharyngeal Cancers
A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University.
Tick Genome Reveals Secrets of a Successful Bloodsucker
NIH-funded study could lead to new tick control methods.
Light Signals from Living Cells
Fluorescent protein markers delivered under high pressure.
Genomic Signature Shared by Five Types of Cancer
National Institutes of Health researchers have identified a striking signature in tumor DNA that occurs in five different types of cancer.
Cat Stem Cell Therapy Gives Humans Hope
By the time Bob the cat came to the UC Davis veterinary hospital, he had used up most of his nine lives.
Crowdfunding the Fight Against Cancer
From budding social causes to groundbreaking businesses to the next big band, crowdfunding has helped connect countless worthy projects with like-minded people willing to support their efforts, even in small ways. But could crowdfunding help fight cancer?
Genetic Mechanism Behind Cancer-Causing Mutations
Researchers at Indiana University has identified a genetic mechanism that is likely to drive mutations that can lead to cancer.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!