Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Identification of Plasma Lipid Biomarkers for Prostate Cancer by Lipidomics and Bioinformatics

Published: Monday, April 08, 2013
Last Updated: Monday, April 08, 2013
Bookmark and Share
This study explores a new approach to seeking biomarkers for prostate cancer and other diseases.

Background:
Lipids have critical functions in cellular energy storage, structure and signaling. Many individual lipid molecules have been associated with the evolution of prostate cancer; however, none of them has been approved to be used as a biomarker. The aim of this study is to identify lipid molecules from hundreds plasma apparent lipid species as biomarkers for diagnosis of prostate cancer.

Methodology/Principal Findings:
Using lipidomics, lipid profiling of 390 individual apparent lipid species was performed on 141 plasma samples from 105 patients with prostate cancer and 36 male controls. High throughput data generated from lipidomics were analyzed using bioinformatic and statistical methods. From 390 apparent lipid species, 35 species were demonstrated to have potential in differentiation of prostate cancer. Within the 35 species, 12 were identified as individual plasma lipid biomarkers for diagnosis of prostate cancer with a sensitivity above 80%, specificity above 50% and accuracy above 80%. Using top 15 of 35 potential biomarkers together increased predictive power dramatically in diagnosis of prostate cancer with a sensitivity of 93.6%, specificity of 90.1% and accuracy of 97.3%. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) demonstrated that patient and control populations were visually separated by identified lipid biomarkers. RandomForest and 10-fold cross validation analyses demonstrated that the identified lipid biomarkers were able to predict unknown populations accurately, and this was not influenced by patient's age and race. Three out of 13 lipid classes, phosphatidylethanolamine (PE), ether-linked phosphatidylethanolamine (ePE) and ether-linked phosphatidylcholine (ePC) could be considered as biomarkers in diagnosis of prostate cancer.

Conclusions/significance:
Using lipidomics and bioinformatic and statistical methods, we have identified a few out of hundreds plasma apparent lipid molecular species as biomarkers for diagnosis of prostate cancer with a high sensitivity, specificity and accuracy.

The article is published online in the journal PLoS ONE and is free to access.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

A Metabolomic Perspective on Coeliac Disease
Metabolomics has proved to be a useful complement for the characterization of several physiological and pathological conditions and offers promises as a clinical tool.
Monday, April 07, 2014
An Alternative Suite of Universal Primers for Genotyping in Multiplex PCR
The new set of universal primers for multiplex genotyping were proven to be efficient and reliable in varied species.
Thursday, March 27, 2014
Application of MALDI-TOF Mass Spectrometry in Clinical Virology
MS methods can be used currently for viral diagnosis but refinement of technology will be an important approach for the introduction of MALDI-TOF in routine viral diagnosis.
Monday, December 16, 2013
Genome-wide DNA Methylation and Gene Expression Analyses of Monozygotic Twins Discordant for IQ Levels
Regarding that MZ twins are identical in genetic composition, the IQ difference could be associated with environmental factors, via epigenetic mechanisms regulations.
Wednesday, October 24, 2012
Genomic Assessment of Human Cumulus Cell Marker Genes
Study includes the investigation of the impact of technical and biological variability such as qPCR series and patients on the level of gene expression.
Thursday, August 02, 2012
Genotyping Performance Between Saliva and Blood-derived Genomic DNAs on the DMET Array
Study also evaluates possible ways to improve the saliva-derived DNA genotyping call rate.
Friday, June 15, 2012
Evaluation of Candidate Reference Genes for Gene Expression Normalization
Study performed in Brassica juncea using real time quantitative RT-PCR.
Monday, May 21, 2012
Reliable and Rapid Characterization of Functional FCN2 Gene Variants Reveals Diverse Geographical Patterns
In this study, scientists genotyped and characterized four FCN2 functional SNP variants in West Africans, South Americans, South East Asians from Vietnam and Caucasians from Germany using an available rapid, reliable and cost effective FRET-RTPCR methodology.
Monday, May 21, 2012
Study of Helicobacter pylori Genotype Status in Saliva, Dental Plaques, Stool and Gastric Biopsy Samples
This study aimed to compare H. pylori cagA and the vacA allelic status among strains isolated from saliva, dental plaque, gastric biopsies and stool samples in the same patient with dyspepsia manifestations in order to evaluate the mode of transmission of H. pylori infection.
Wednesday, May 16, 2012
Serious Bacterial Infection Biomarkers Uncovered using Transcriptomic Analysis
Novel biomarker combination improves the diagnosis of serious bacterial infections in Malawian children.
Monday, May 14, 2012
Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
In this work we have validated candidate reference genes for accurate normalization of RT-qPCR data in cyanobacteria. Several genes were selected and their transcription analyzed in three morphological distinct organisms.
Monday, April 23, 2012
DNA Methylation Analysis of Bone Marrow Cells at Diagnosis of Acute Lymphoblastic Leukemia and at Remission
The aim of this study was to identify genes that display aberrant DNA methylation independently of cytogenetic ALL subtype for further mechanistic studies of ALL.
Monday, April 16, 2012
Integrated Analyses of microRNAs Demonstrate Their Widespread Influence on Gene Expression in High-Grade Serous Ovarian Carcinoma
The Cancer Genome Atlas (TCGA) Network recently catalogued the molecular aberrations in 487 high-grade serous ovarian cancers. This study surveys the miRNAs, in the context of their predicted gene targets.
Monday, April 16, 2012
The Next Phase of Life-Sciences Spaceflight Research: Harnessing the Power of Functional Genomics
This article discusses potential applications of RNAi and GFP in spaceflight studies and the ramifications of these experiments for the future of space life-sciences research.
Monday, April 02, 2012
Keratinocyte Growth Factor Induces Gene Expression Signature Associated with Suppression of Malignant Phenotype of Cutaneous Squamous Carcinoma Cells
This study examined the effect of KGF on cutaneous SCC cells. The results show that although most SCC cell lines express KGFR, KGF does not stimulate their proliferation.
Thursday, March 22, 2012
Scientific News
Poor Survival Rates in Leukemia Linked to Persistent Genetic Mutations
For patients with an often-deadly form of leukemia, new research suggests that lingering cancer-related mutations – detected after initial treatment with chemotherapy – are associated with an increased risk of relapse and poor survival.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Growing Hepatitis C in the Lab
Recent discovery allows study of naturally occurring forms of hepatitis C virus (HCV) in the lab.
Signature of Microbiomes Linked to Schizophrenia
Studying microbiomes in throat may help identify causes and treatments of brain disorder.
Study Identifies the Off Switch for Biofilm Formation
New discovery could help prevent the formation of infectious bacterial films on hospital equipment.
Genetic Overlapping in Multiple Autoimmune Diseases May Suggest Common Therapies
CHOP genomics expert leads analysis of genetic architecture, with eye on repurposing existing drugs.
Fat in the Family?
Study could lead to therapeutics that boost metabolism.
Combo Tool
Joining molecular components expands ability to manipulate genes in specific cell types.
Team Identifies Structure of Tumor-Suppressing Protein
An international group of researchers led by Carnegie Mellon University physicists Mathias Lösche and Frank Heinrich have established the structure of an important tumor suppressing protein, PTEN.
Genes Associated With Improved Survival for Pancreatic Cancer Patients
Use of non-invasive liquid biopsies could predict in which patients the cancer could recur following surgery.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!