Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
Become a Member | Sign in
Home>News>This Article

Merck Millipore Launches MILLIPLEX® MAP Kits

Published: Tuesday, May 07, 2013
Last Updated: Tuesday, May 07, 2013
Bookmark and Share
Enables detection of multiple pluripotency markers in a single measurement.

Merck Millipore has announced availability of the MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kits, which enable the analysis of many different pluripotency biomarkers in a single measurement.

These kits are the first commercially available multiplexed protein biomarker assays based on the Luminex® xMAP® platform for this important research area.

This new application of trusted bead-based multiplex technology gives researchers rapid feedback on the potency status of stem cell cultures and offers a significant advantage over existing techniques, which detect a limited number of proteins with a semi-quantitative output for a single sample.

Merck Millipore's new MILLIPLEX® MAP kits examine protein biomarkers that indicate the potency of stem cells, enabling pluripotent stem cells to be distinguished from multipotent or differentiating cells.

These new kits save time and resources by accurately detecting large numbers of pluripotency markers in one measurement. Kits can be used on cultures of induced pluripotent stem cells, human embryonic stem cells, cancer stem cells or cancer cell lines.

Two kits are available for assessing stem cell pluripotency. The MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kit 1 monitors the expression of the transcription factors Oct 3/4, Sox2, Nanog and c-Myc, which play critical roles in the maintenance of pluripotency and self-renewal in stem cells.

The MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kit 2 contains seven additional pluripotency-related markers, including a transcription factor, a translational regulator, transmembrane proteins and cell surface glycostructures.

These assays are essential for assessing the potency of stem cell cultures in studies ranging from cancer and developmental biology research to bioprocess monitoring. MILLIPLEX® MAP assays make it easy to analyze both cell surface and intracellular proteins.

"The MILLIPLEX® MAP Stem Cell Pluripotency Kit 1 is a valuable tool for stem cell research. We obtained very interesting and promising results," said Frank Edenhofer, Ph.D., Head of the Stem Cell Engineering Group, Institute of Reconstructive Neurobiology, University of Bonn, LIFE & BRAIN Center. "It is noteworthy that this assay enabled us to observe even early onset of differentiation in iPS cell cultures."

"Stem cells are of great interest for regenerative medicine and drug discovery efforts, and researchers must be able to effectively characterize the potency status of pluripotent stem cells to advance their progress toward clinical use," said Linda Meeh, Ph.D., Director of Marketing for Immunoassays and Multiplexing.

Meeh continued, "MILLIPLEX® MAP kits offer a quick and simple way to achieve this characterization, generating a comprehensive assessment of the overall quality of cell cultures."

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Princeton’s Dr Bonnie Bassler Receives 2014 Alice C. Evans Award
Merck Millipore congratulates winner of the Alice C. Evans award for leadership in clinical microbiology.
Thursday, May 22, 2014
Merck Millipore Announces Incoming CEO and President
Udit Batra, Ph.D. will be responsible for the organization’s strategic direction to drive growth and product innovation as well as oversight of the worldwide operations.
Tuesday, April 15, 2014
Merck Millipore Announces New Genomic Biomarker Services
Genetic and epigenetic technologies added to established CRO services for biomarker development, validation and testing.
Wednesday, December 05, 2012
Scientific News
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Mathematical Model Forecasts the Path of Breast Cancer
Chances of survival depend on which organs breast cancer tumors colonize first.
Ancient Viral Molecules Essential for Human Development
Genetic material from ancient viral infections is critical to human development, according to researchers at the Stanford University School of Medicine.
Measuring microRNAs in Blood to Speed Cancer Detection
A simple, ultrasensitive microRNA sensor holds promise for the design of new diagnostic strategies and, potentially, for the prognosis and treatment of pancreatic and other cancers.
Personalized Drug Screening for Multiple Myeloma Patients
A personalized method for testing the effectiveness of drugs that treat multiple myeloma may predict quickly and more accurately the best treatments for individual patients with the bone marrow cancer.
Metabolic Profiles Distinguish Early Stage Ovarian Cancer with Unprecedented Accuracy
Studying blood serum compounds of different molecular weights has led scientists to a set of biomarkers that may enable development of a highly accurate screening test for early-stage ovarian cancer.
New Way to Force Stem Cells to Become Bone Cells
Potential therapies based on this discovery could help people heal bone injuries or set hardware, such as replacement knees and hips.
Promise of Newborn Stem Cells to Revolutionize Clinical Practice
In this article Shweta Sharma, PhD, discusses the potential of an Umbilical Cord Blood bank as an untapped source of samples for research and clinical trials.
New Anti-Malarial Drug Screening Model
University of South Florida researchers demonstrate novel chemogenomic profiling to identify drug targets for the most lethal strain of malaria.
Coronavirus Breakthrough
Protein mutation affects spread and virulence of respiratory virus.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos