Bio-Rad Laboratories, Inc. announce the launch of its SsoAdvanced universal supermixes that deliver superior real-time PCR (qPCR) performance under any reaction conditions on any qPCR instrument.
Both supermixes include Bio-Rad’s patented Sso7d fusion enzyme, which provides the highest-quality data from the most challenging sample types. The enzyme’s performance is aided by an advanced formulation of surfactants, detergents, stabilizers, and other proprietary components, allowing the supermixes to be used across a wide range of cycling conditions, primer concentrations, and temperatures.
The meaning of the term “universal” is twofold:
• These supermixes are compatible with all qPCR platforms. Multiple passive reference dyes blended in Bio-Rad’s supermixes are particularly useful for researchers using qPCR instruments that require ROX fluorescent dyes. The supermixes eliminate the need to change reagents between experiments and instruments.
• These supermixes yield optimal performance across a broad range of challenging sample types and difficult target sequences, so they can be used with wide variety of qPCR applications, including gene expression studies, ChIP analyses, genotyping, and more.
The SYBR® Green supermix is optimized for dye-based qPCR and contains SYBR® Green I, whereas the probes supermix is formulated for probe-based qPCR (simplex or duplex). The SsoAdvanced universal supermixes complement Bio-Rad’s iTaq™ universal supermixes, which are suitable for most samples and targets.