Corporate Banner
Satellite Banner
Genotyping & Gene Expression
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

iTaq Universal One-Step Kits for RT-qPCR

Published: Wednesday, August 21, 2013
Last Updated: Wednesday, August 21, 2013
Bookmark and Share
Bio-Rad Laboratories, Inc. announced the launch of two iTaq universal one-step kits that deliver superior data precision and highly efficient RT-qPCR performance under most reaction conditions on any qPCR instrument.

The new iTaq universal one-step kits include the iTaq universal SYBR® Green one-step kit and the iTaq universal probes one-step kit. Both kits come as a 2x concentrated, ready-to-use reaction mix with iScript™ reverse transcriptase optimized for RT-qPCR. 

The one-step kits are universal due to their broad instrument compatibility and the ability to tolerate a wide range of reaction and protocol conditions. The multiple passive reference dyes included in their formulation are particularly useful for researchers using qPCR instruments that require ROX fluorescent dyes. In addition, the combination of RNase H+ iScript reverse transcriptase and antibody-mediated hot-start iTaq DNA polymerase in one reaction improves PCR efficiency, dynamic range, data precision, sensitivity, and specificity.

The iTaq universal SYBR® Green one-step kit includes SYBR® Green I dye and Bio-Rad’s patented RT inhibitor reducer. The iTaq universal probes one-step kit allows researchers to combine multiple targets in one well (multiplexing), improving data precision and reducing costs.

 Key benefits of the iTaq universal one-step kits include:

• Uncompromised data with fast cycling — obtain highly efficient data in 55 minutes, which includes the reverse transcription reaction, using fast cycling conditions

• Optimized buffer formulation — achieve efficient amplification of challenging templates, cell lysates, and difficult target sequences 

• Instant activation — complete polymerase activation in 30 seconds for greater reaction specificity and superior data

Bio-Rad’s iTaq universal one-step kits can be used for gene expression analysis, mutation detection, genetically modified organism (GMO) characterization, genetic profiling, and other genomics applications.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,600+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Higher Frequency of Huntington's Disease Mutations Discovered
University of Aberdeen study shows that the gene change that causes Huntington's disease is much more common than previously thought.
Revealing the Genetic Causes of Bowel Cancer
A landmark study has given the most detailed picture yet of the genetics of bowel cancer — the UK's fourth most common cancer.
Tumor Cells Develop Predictable Characteristics
Scientists have discovered that cancer cells at the edge of a tumor that are close to the surrounding environment are predictably different from the cells within the interior of the tumor.
New Imaging Method Reveals Nanoscale Details about DNA
Enhancement to super-resolution microscopy shows orientation of individual molecules, providing a new window into DNA’s structure and dynamics.
Genetic Research Can Significantly Improve Drug Development
With drug development costs topping $1.2bn (£850 million) to get a single treatment to the point it can be sold and used in the clinic, could genetic analysis save hundreds of millions of dollars?
Diagnosing Systemic Infections Quickly, Reliably
Team develop rapid and specific diagnostic assay that could help physicians decide within an hour whether a patient has a systemic infection and should be hospitalized for aggressive intervention therapy.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Scoliosis Linked to Disruptions in Spinal Fluid Flow
A new study in zebrafish suggests that irregular fluid flow through the spinal column brought on by gene mutations is linked to a type of scoliosis that can affect humans during adolescence.
A New Tool Brings Personalized Medicine Closer
Scientists from EPFL and ETHZ have developed a powerful tool for exploring and determining the inherent biological differences between individuals, which overcomes a major hurdle for personalized medicine.
Blood Test That Detects Early Alzheimer’s Disease
A research team, led by Dr. Robert Nagele from Rowan University School of Osteopathic Medicine and Durin Technologies, Inc., has announced the development of a blood test that leverages the body’s immune response system to detect an early stage of Alzheimer’s disease – referred to as the mild cognitive impairment (MCI) stage – with unparalleled accuracy.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!